Ameliorative Effect of Saccharomyces cerevisiae JKSP39 on Fusobacterium nucleatum and Dextran Sulfate Sodium-Induced Colitis Mouse Model

Abstract

The aim of this study was to evaluate the ability of the Saccharomyces cerevisiae strain with probiotic properties isolated from Tibetan kefir grains to ameliorate Fusobacterium nucleatum (Fn) infection and dextran sulfate sodium (DSS) treatment-induced murine model of colitis. The tolerance to simulated gastrointestinal conditions, hydrophobicity test, autoaggregation assay, and the antioxidant effect of strains was used to screen one strain with colonization and probiotic potential. The murine model of colitis was established by giving 109 cfu Fn 3 weeks by intragastric administration and 3% DSS in water 1 week before sacrifice. The results indicated that S. cerevisiae JKSP39 (SC) possessed optimal probiotic characteristics in vitro. Supplementation with SC increased the body weight and the expression of anti-inflammatory cytokines (IL-4 and IL-10), while decreasing the disease activity index score and expression of proinflammatory cytokines (TNF-α, IL-6, and IL-17F) in mice undergoing experimental colitis as compared with the colitis model group. Additionally, tight junction proteins and the number of goblet cells per crypt were significantly increased in the SC group, which indicated that the gut barrier was well repaired. The mechanism of SC ameliorating Fn-DSS-induced colitis could be related to the decreased levels of reactive oxygen species (myeloperoxidase, total superoxide dismutase, catalase, H2O2, and malondialdehyde) in the colon, the inhibition of endoplasmic reticulum stress, and the regulation of gut microbiota.

Anti-Inflammatory, Barrier Maintenance, and Gut Microbiome Modulation Effects of Saccharomyces cerevisiae QHNLD8L1 on DSS-Induced Ulcerative Colitis in Mice

“…Yeast treatments, except for D. hansenii QSCLS6L3, could restore these changes in colitis ( Figure 4 ). In particular, S. cerevisiae QHNLD8L1 could significantly decrease TNF-α and increase IL-10 concentrations, which was consistent with S. cerevisiae JKSP39 in previous reports [ 15 ]. MPO activity is a classic biomarker used to evaluate inflammation in colitis and reflects the degree of neutrophil infiltration [ 40 ].…”

“…Yeasts may possess potential probiotic characteristics; however, studies on their probiotic functions in human health are rare. Although several yeasts have shown promising effects in the treatment of colitis [ 14 , 15 ], it is still unknown whether other yeast strains have a therapeutic potential. Additionally, a few researchers have explored the mechanisms by which yeasts regulate the immune response in UC.…”

Regulation of Gut Microbiota and Microbial Metabolome of Kefir Supernatant against Fusobacterium nucleatum and DSS-Coinduced Colitis

“…The experiment flow was referred to our previous published study. 30 Analysis of Gut Microbiota. The feces of mice were collected the day before the mice were sacrificed.…”

Neonatal Calf Diarrhea and Gastrointestinal Microbiota: Etiologic Agents and Microbiota Manipulation for Treatment and Prevention of Diarrhea

“…In calves, the administration of probiotics containing yeast increases their grain consumption. This may be due to their ability to facilitate fiber digestion, which aids in gut development [ 69 , 70 ]. Yeast of Saccharomyces cerevisiae (SCY) origin is used in different food-producing animals to improve performance and health conditions [ 70 ].…”