Ameliorative effect of polyphenols from Padina boergesenii against ferric nitrilotriacetate induced renal oxidative damage: With inhibition of oxidative hemolysis and in vitro free radicals

Abstract

The aim of this study was to evaluate the antioxidant activities of diethyl ether (DEE) and methanol (M) extracts from brown alga Padina boergesenii using in vitro and in vivo antioxidant assay, which may help to relate the antioxidant properties with the possible outline of its ameliorative effect. M extract showed higher radical scavenging activity through ferric reducing antioxidant power 139.11 µmol tannic acid equivalent/g; DPPH 71.32 ± 0.56%; deoxyribose radical 88.31 ± 0.47%, and total antioxidant activity 0.47 ± 0.02 mg ascorbic acid equivalents/g. Oxidative red blood cell (RBC) hemolysis inhibition rate was significantly higher in M extract (150 mg/kg body weight) in reference to total phenolic content (r = 0.935). Rats administered with DEE and M extracts (150 mg/kg body weight) for seven days before the administration of ferric nitrilotriacetate (9 mg of Fe/mg/kg bodyweight). Rats pretreated with extracts significantly changed the level of renal microsomal lipid peroxidation, glutathione, and antioxidant enzymes in post-mitochondrial supernatant (P < 0.05). Ameliorative effect of extracts against renal oxidative damage was evident in rat kidney through changes in necrotic and epithelial cells. HPTLC technique has identified the presence of rutin with reference to retardation factor (Rf ) in both the extracts. These findings support the source of polyphenols (rutin) from P. boergesenii had potent antioxidant activity; further work on isolation of bioactive compounds can be channeled to develop as a natural antioxidant.

Protective Effect of Sundakai (Solanum torvum) Seed Protein (SP) Against Oxidative Membrane Damage in Human Erythrocytes

“…Dietary antioxidants have been shown to protect the cells from damage caused by oxidative stress and to fortify the defense system against degenerative diseases (Clarke et al 2008;Riccioni et al 2008). Several natural antioxidants and phytonutrients present in dietary sources reduced the oxidative stress and oxidative stress-induced hemolysis (Tulipani et al 2011(Tulipani et al , 2014Widen et al 2012;Rajamani et al 2014;Phrueksanan et al 2014). …”

Polyphenols from brown alga, Padina boergesenii (Allendar & Kraft) decelerates renal cancer growth involving cell cycle arrest and induction of apoptosis in renal carcinoma cells

“…Cellular and molecular studies have reported that algal products are potential antioxidants suggested to prevent cancer metastasis . We reported in our earlier study about the ameliorative effect of polyphenols and inhibition of oxidative hemolysis being identified in crude extract from this alga . The alga was extracted and partitioned through silica gel with combination of different solvent system such as methanol:ethylacetae (ME) (1:3), chloroform:ethylacetate (1:3), and butanol:ethylacetate (1:3) five fractions (F1‐F5) from each system was collected and screened for in vitro radical scavenging activity (data not shown).…”