Amended recombinant cells (ARCs™): An economical and surprisingly effective production and delivery vehicle for recombinant bovine IFN-γ

Gaertner, Frank; Babiuk, Lorne A.; Moorlehem, Elaine A. Van; Beskorwayne, Terry; Lee, Stacey L.; Shutter, Robert W.; Armstrong, Janna M.; Griebel, Philip

doi:10.1016/j.jconrel.2005.06.008

Cited by 7 publications

(2 citation statements)

References 38 publications

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“…Furthermore, only the WMS group displayed significantly elevated serum haptoglobin levels on both day 3 and 4 post-BHV-1 infection (Figure 2c). Fever and serum haptoglobin are acute-phase responses induced by a variety of cytokines, including IL-1, IL-6 and IFN-γ [32,33,40]. Therefore, IFN-α and - γ levels in nasal secretions were measured to quantify host response to viral infection.…”

Section: Resultsmentioning

confidence: 99%

Stress significantly increases mortality following a secondary bacterial respiratory infection

Hodgson

Aich

Stookey

et al. 2012

Vet Res

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A variety of mechanisms contribute to the viral-bacterial synergy which results in fatal secondary bacterial respiratory infections. Epidemiological investigations have implicated physical and psychological stressors as factors contributing to the incidence and severity of respiratory infections and psychological stress alters host responses to experimental viral respiratory infections. The effect of stress on secondary bacterial respiratory infections has not, however, been investigated. A natural model of secondary bacterial respiratory infection in naive calves was used to determine if weaning and maternal separation (WMS) significantly altered mortality when compared to calves pre-adapted (PA) to this psychological stressor. Following weaning, calves were challenged with Mannheimia haemolytica four days after a primary bovine herpesvirus-1 (BHV-1) respiratory infection. Mortality doubled in WMS calves when compared to calves pre-adapted to weaning for two weeks prior to the viral respiratory infection. Similar results were observed in two independent experiments and fatal viral-bacterial synergy did not extend beyond the time of viral shedding. Virus shedding did not differ significantly between treatment groups but innate immune responses during viral infection, including IFN-γ secretion, the acute-phase inflammatory response, CD14 expression, and LPS-induced TNFα production, were significantly greater in WMS versus PA calves. These observations demonstrate that weaning and maternal separation at the time of a primary BHV-1 respiratory infection increased innate immune responses that correlated significantly with mortality following a secondary bacterial respiratory infection.

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Section: Resultsmentioning

confidence: 99%

Stress significantly increases mortality following a secondary bacterial respiratory infection

Hodgson

Aich

Stookey

et al. 2012

Vet Res

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“…The NF samples were centrifuged at 1,000 X g for 5 minutes, and a 0.5-mL aliquot of each sample was transferred into a cryovial that contained 50 µL of 100X protease inhibitor j to minimize IFN degradation. 17 Samples were stored at -20°C until analyzed. The alternate nostril was used for each successive specimen collection.…”

Section: Sample Collectionmentioning

confidence: 99%

Innate and acquired immune responses of colostrum-fed neonatal Holstein calves following intranasal vaccination with two commercially available modified-live virus vaccines

Midla

Hill

Engen

et al. 2021

javma

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B ovine respiratory disease continues to impair animal welfare and cause substantial economic losses owing to morbidity, reduced performance, and death of cattle in both the beef and dairy industries. 1 Total prevention of BRD remains an elusive goal. Vaccination is an important adjunct to improvements in management, nutrition, and biosecurity. For some animals, such as young calves with circulating maternal antibodies, vaccine administration by the IN route may result in a superior immune response than vaccine administration by other routes. Indeed,

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Special Issues around Veterinary Vaccines

Adams

Babiuk

McGavin

et al. 2009

Vaccines for Biodefense and Emerging and Neglected Diseases

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Amended recombinant cells (ARCs™): An economical and surprisingly effective production and delivery vehicle for recombinant bovine IFN-γ

Cited by 7 publications

References 38 publications

Stress significantly increases mortality following a secondary bacterial respiratory infection

Stress significantly increases mortality following a secondary bacterial respiratory infection

Innate and acquired immune responses of colostrum-fed neonatal Holstein calves following intranasal vaccination with two commercially available modified-live virus vaccines

Special Issues around Veterinary Vaccines

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