Amides as cryoprotectants for the freezing of Brycon orbignyanus sperm

Perry, Carolina Trindade; Corcini, Carine Dahl; Anciuti, Andréia Nobre; Otte, Marina Vianna; Soares, Sara Lorandi; Garcia, Juan Ramón Esquivel; Muelbet, Juan Ramon Esquivel; Varela, Antônio Sérgio

doi:10.1016/j.aquaculture.2019.03.015

Cited by 14 publications

(11 citation statements)

References 48 publications

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“…The enhancement of cryoprotection observed with the use of LDL-P micelles could be associated with the changes in the physicochemical properties of these micelles, such as changes in the diameter and surface electricity. These physicochemical properties are closely associated with the dynamics of the interface between the nanoparticles and the lipid bilayers, in addition to the dynamics of the solid–liquid interface, as well as with the changes occurring during the interaction of the particles with the suspension medium and biological molecules ( 3 , 4 ), including the semen plasma proteins ( 5 ).…”

Section: Discussionmentioning

confidence: 99%

“…This is mainly due to the high surface area at the nano-level, which allows an intense interaction of the nanoparticles with the matrix they are introduced in ( 2 ). In animal reproduction, certain metal-conjugated nanoparticles ( 3 ), natural extracts ( 4 ), and vitamin E nanoemulsions ( 5 ) with antioxidant properties are reported to be among the most promising candidates for application in sperm cryopreservation ( 6 ).…”

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confidence: 99%

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Probe ultrasonification of egg yolk plasma forms low-density lipoprotein nanoparticles that efficiently protect canine semen during cryofreezing

Silva¹,

Corcini²,

Camelo³

et al. 2022

Journal of Biological Chemistry

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Probe ultrasonification of egg yolk plasma forms low-density lipoprotein nanoparticles that efficiently protect canine semen during cryofreezing

Silva¹,

Corcini²,

Camelo³

et al. 2022

Journal of Biological Chemistry

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“…Several studies have demonstrated that not only storage (Merino et al 2017) but also vitrification (Figueroa et al 2013;Zilli et al 2018) and cryopreservation alter mitochondrial integrity and normal functioning (Ogier De Baulny et al 1997;He & Woods 2004;Cabrita et al 2005a;Viveiros et al 2015;Figueroa et al, 2016Figueroa et al, , 2017Figueroa et al, , 2019Perry et al 2019). Damage at this level reduces not only the percentage of motile spermatozoa, but also the duration of sperm motility (He & Woods 2004) and the fertility.…”

Section: Mitochondrial Damagementioning

confidence: 99%

“…2015; Figueroa et al, 2016, 2017, 2019; Perry et al . 2019). Damage at this level reduces not only the percentage of motile spermatozoa, but also the duration of sperm motility (He & Woods 2004) and the fertility.…”

Section: Mitochondrial Damagementioning

confidence: 99%

Oxidative stress and use of antioxidants in fish semen cryopreservation

Sandoval‐Vargas

Jiménez

González

et al. 2020

Reviews in Aquaculture

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Reactive oxygen species (ROS) have been proposed as one of the main causes of the impairment of fish spermatozoa integrity and functionality during cryopreservation. The high content of unsaturated fatty acids in sperm cells and the low antioxidant capacity of diluted semen are key factors in making sperm cells susceptible to ROS attacks. For this reason, some recent studies have determined the antioxidant status of the seminal plasma and spermatozoa of fish species. Additionally, some studies have evaluated the effects of antioxidants on post-thaw sperm quality. Although ROS are certainly involved in sperm damage, other factors, such as ice crystal formation, seem to play a crucial role in cryodamage. This challenge has not yet been resolved because both the endogenous antioxidant capacity of the semen and its response to different supplementation practices seem to present specific inter-and intraspecies characteristics and effects. This review summarises knowledge on antioxidant defence and oxidative stress in fish semen, as well as antioxidant supplementation in cryopreservation media, in order to establish perspectives for future studies.

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“…Dimethyl sulfoxide (DMSO), glycerol and methyl glycol (MG) are common intracellular cryoprotectants used for cryopreservation of fish spermatozoa (Viveiros et al, ). Indeed, alcohol‐based cryoprotectants are widely used for cryopreservation of sperm from freshwater fish: Piracanjuba ( Brycon orbignyanus ; Perry et al, ), Tambaqui ( Colossoma macropomum ; Garcia et al, ; Varela Junior et al, ), Curimba ( Prochilodus lineatus ; Miliorini et al, ; Viveiros et al, ), Pacu ( Piaractus mesopotamicus ; Pires et al, ).…”

Section: Introductionmentioning

confidence: 99%

Cryopreservation of sperm in annual fish Austrolebias minuano (Cyprinodontiformes; Rivulidae)

et al. 2019

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The effects of the cryoprotectants dimethyl sulfoxide, glycerol and methyl glycol at different concentrations on Austrolebias minuano sperm quality parameters were evaluated in this study. The cellular kinetic parameters, determined using flow cytometry, indicated the best results with the samples cryopreserved with 7.5% methyl glycol. Dimethyl sulfoxide concentrations of 7.5% and glycerol concentrations of 10%, 12.5% and 15% demonstrated the least benefit across all evaluated sperm kinetic parameters. When assessed using flow cytometry, a concentration of 7.5% methyl glycol similarly showed better sperm kinetic parameters than 12.5% dimethyl sulfoxide. We conclude that cryoprotectants, especially methyl glycol, are effective for the preservation of sperm quality in A. minuano. However, high sensitivity of spermatozoa against glycerol was observed in these studies; thus, it is not recommended for cryopreservation purposes. K E Y W O R D Scryoprotectants, extinction, killifish, preservation

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Amides as cryoprotectants for the freezing of Brycon orbignyanus sperm

Cited by 14 publications

References 48 publications

Probe ultrasonification of egg yolk plasma forms low-density lipoprotein nanoparticles that efficiently protect canine semen during cryofreezing

Probe ultrasonification of egg yolk plasma forms low-density lipoprotein nanoparticles that efficiently protect canine semen during cryofreezing

Oxidative stress and use of antioxidants in fish semen cryopreservation

Cryopreservation of sperm in annual fish Austrolebias minuano (Cyprinodontiformes; Rivulidae)

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