Amine-based aqueous polymers for the simultaneous titration and extraction of lactic acid in aqueous two-phase systems

Practical application of aqueous two-phase partition to process development for the recovery of biological products

“…Although, further studies to address the potential application of ATPS to process integration as a primary step for the recovery of intracellular proteins are essential, it is clear that process economics benefits are associated to the reduction of unit operations by the proposed approach of process integration. [36] EOPO-dextran Extractive fermentation for the recovery of lactic acid from Lactococcus lactis [49,50] PEG-phosphate Recovery of viral coat proteins from recombinant E. coli [16] Preparation of highly purified fractions of small inclusion bodies [53] Recovery of aroma compounds under product inhibition conditions [17] PEG-sulphate Drowning-out crystallisation of sodium sulphate [45] Recovery of metal ions from aqueous solutions [47] Recovery of food coloring dyes from textile plant wastes [46] Partition of small organic molecules [48] EOPO, ethylene oxide propylene oxide.…”

“…The problem of product inhibition exhibited in the production of certain aroma compounds (such as 6-penthyl alpha pyrone produced by T. harzianum [17]) can be addressed by the continuous removal of the product of interest from the fermentation broth using and extractive phase with ATPS. The bio-compatibility of ATPS facilitate the integration with fermentation step to address a problem not specific to the production of aroma compounds (see lactic acid production in references [49,50]). …”

Effective extraction of elastase from Bacillus sp. fermentation broth using aqueous two-phase system

“…Aqueous two-phase system (ATPS) is widely used in biochemistry and biotechnology for purification of proteins (Diamond and Hsu, 1992;Balasubramaniam et al, 2003), enzymes (Pan et al, 2001;Bim and Franco, 2000), amino acids (Li et al, 2002), antibiotics (Yang et al, 1994;Guan et al, 1994), nucleic acids (Ohlsson et al, 1978;Kimura, 2000), aroma compounds (Marco et al, 2000) and lactic acid (Planas et al, 1998). ATPS is mainly composed of two-incompatible polymers (e.g.…”

Novel polymer-polymer conjugates for recovery of lactic acid by aqueous two-phase extraction

“…The partitioning of the conjugate to the top phase increased K lac . The effect of phosphate on K lac has been described previously and related to the partitioning of PEI in EOPO-DEX ATPS (Planas et al, 1998). The extent to which the effects of phosphate on K lac are related to the ionic balance between the phases needs further investigation.…”

“…The highest value of K lac in EOPO-DEX systems, (i.e., K lac 1.3) was observed at pH 2 and decreased to 0.4 at pH 5. When PEI (MW ≈ 2 kDa) was included in EOPO-DEX systems, K lac decreased to 0.09 at pH 6 (Planas et al, 1998). PEI has been used previously as a phase-forming polymer, giving rise to ATPS when mixed with PEG, dextran, or (hydroxyethyl) cellulose (HEC) (Dissing and Mattiasson, 1993).…”