1945
DOI: 10.1139/f42-042
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Amines in Fish Muscle: I. Colorimetric Determination of Trimethylamine as the Picrate Salt

Abstract: A sensitive accurate colorimetric method for trimethylamine determination is presented, based on the extraction with toluene of an alkaline sample containing 0.002 to 0.02 mg. trimethylamine nitrogen, and the formation of the yellow coloured picrate by mixing with a picric acid reagent. The application of the method in fishery products and effects of interfering substances have been investigated.

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Cited by 200 publications
(87 citation statements)
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“…This reagent, otherwise used for the determination of phenols, gives a blue color with TMA (8) but not with ammonium, mono-or dimethylamine salts. Since the phenols of the urine are not aerated under the experimental conditions, the color reaction can be considered specific for TMA.…”
Section: Methodsmentioning
confidence: 99%
“…This reagent, otherwise used for the determination of phenols, gives a blue color with TMA (8) but not with ammonium, mono-or dimethylamine salts. Since the phenols of the urine are not aerated under the experimental conditions, the color reaction can be considered specific for TMA.…”
Section: Methodsmentioning
confidence: 99%
“…Regarding color, morphological and biochemical differences are observed between two types of muscles, ordinary (white) and blood (red), being morphologically characterized as: ordinary muscle presenting large muscle fibers, large proportion of myofibril/ sarcoplasm, a few external and internal membranes and scattered distribution of blood vessels; blood muscle characterized by being composed of small muscle fibers, a small proportion of myofibril/ sarcoplasm, many external and internal membranes and compact distribution of blood vessels. Biochemically the red muscle has a higher proportion of sarcoplasmic protein, high glycogen and stroma content, higher lipids, taurine (sulfonic amino acid), iron, extractive nitrogen (mainly histidine) compared to the ordinary muscle (Dyer 1945, Stansky 1962.…”
Section: Resultsmentioning
confidence: 99%
“…A amônia liberada foi coletada em solução de ácido bórico (Sigma) com indicadores e quantifi cada por titulometria com ácido clorídrico padronizado. Analisou-se a concentração de trimetilamina (TMA) colorimetricamente como picrato de TMA pelo método de Dyer (1945) …”
Section: Avaliação Químicaunclassified