Amino acid catabolism in the nematodes<i>Heligmosomoides polygyrus</i>and<i>Panagrellus redivivus</i>2. Metabolism of the carbon skeleton

Grantham, B D; Barrett, John

doi:10.1017/s0031182000081208

Cited by 7 publications

(4 citation statements)

References 22 publications

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“…2 e,f). These data are also in good agreement with the findings of analogous intravital experiments 12 .…”

Section: Resultssupporting

confidence: 92%

“…Besides, a similar pattern of NADPH oxidases activation, i.e. higher in epithelial cells than in cells of the lamina propria, was detected both in explanted duodenum, as presented here, and in the small intestine of living mice 12 . In this way, we validated the experimental model for analyzing the cross-link between host and parasite, along the metabolic axis.…”

Section: Discussionsupporting

confidence: 84%

“…Up to now, information on metabolism of live parasites (e.g. nematodes) in the host intestine was mostly acquired using bulk biochemical approaches, without spatial or temporal specification 12 .…”

Section: Introductionmentioning

confidence: 99%

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NAD(P)H fluorescence lifetime imaging of live intestinal nematodes reveals metabolic crosstalk between parasite and host

Liublin

Leben

Lindquist

et al. 2022

Sci Rep

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Infections with intestinal nematodes have an equivocal impact: they represent a burden for human health and animal husbandry, but, at the same time, may ameliorate auto-immune diseases due to the immunomodulatory effect of the parasites. Thus, it is key to understand how intestinal nematodes arrive and persist in their luminal niche and interact with the host over long periods of time. One basic mechanism governing parasite and host cellular and tissue functions, metabolism, has largely been neglected in the study of intestinal nematode infections. Here we use NADH (nicotinamide adenine dinucleotide) and NADPH (nicotinamide adenine dinucleotide phosphate) fluorescence lifetime imaging of explanted murine duodenum infected with the natural nematode Heligmosomoides polygyrus and define the link between general metabolic activity and possible metabolic pathways in parasite and host tissue, during acute infection. In both healthy and infected host intestine, energy is effectively produced, mainly via metabolic pathways resembling oxidative phosphorylation/aerobic glycolysis features. In contrast, the nematodes shift their energy production from balanced fast anaerobic glycolysis-like and effective oxidative phosphorylation-like metabolic pathways, towards mainly anaerobic glycolysis-like pathways, back to oxidative phosphorylation/aerobic glycolysis-like pathways during their different life cycle phases in the submucosa versus the intestinal lumen. Additionally, we found an increased NADPH oxidase (NOX) enzymes-dependent oxidative burst in infected intestinal host tissue as compared to healthy tissue, which was mirrored by a similar defense reaction in the parasites. We expect that, the here presented application of NAD(P)H-FLIM in live tissues constitutes a unique tool to study possible shifts between metabolic pathways in host-parasite crosstalk, in various parasitic intestinal infections.

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“…2 e,f). These data are also in good agreement with the findings of analogous intravital experiments 12 .…”

Section: Resultssupporting

confidence: 92%

Section: Discussionsupporting

confidence: 84%

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NAD(P)H fluorescence lifetime imaging of live intestinal nematodes reveals metabolic crosstalk between parasite and host

Liublin

Leben

Lindquist

et al. 2022

Sci Rep

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show abstract

Section: Introductionmentioning

confidence: 99%

NAD(P)H Fluorescence Lifetime Imaging of Live Intestinal Nematodes Reveals Metabolic Crosstalk Between Parasite and Host

Liublin

Leben

Liebeskind

et al. 2021

Preprint

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Infections with intestinal nematodes have an ambivalent impact: they represent a burden for human health and animal husbandry, but, at the same time, may ameliorate auto-immune diseases due to the immunomodulatory effect of the parasites. Thus, it is key to understand how intestinal nematodes arrive and persist in their luminal niche and interact with the host over long periods of time. The basic mechanism ruling over parasite and host cellular and tissue functions, the metabolism, was largely neglected in the study of intestinal nematode infections. Here we use NADH (nicotinamide adenine dinucleotide) and NADPH (nicotinamide adenine dinucleotide phosphate) fluorescence lifetime imaging of explanted murine duodenum infected with the natural nematode Heligmosomoides polygyrus and define the link between general metabolic and specific enzymatic activity in parasite and host tissue, during acute infection. In both healthy and infected host intestine, energy is effectively produced, mainly via oxidative phosphorylation. In contrast, the nematodes shift their energy production from balanced fast anaerobic glycolysis and effective oxidative phosphorylation, towards mainly anaerobic glycolysis, back to oxidative phosphorylation during the different life cycle phases in the submucosa versus the intestinal lumen. Additionally, we found an increased NADPH oxidase (NOX) enzymes-dependent oxidative burst in infected intestinal host tissue as compared to healthy tissue, which was mirrored by a similar defense reaction in the parasites. We expect that, the here presented application of in vivo NAD(P)H-FLIM constitutes a unique tool to study metabolic host-parasite crosstalk, in various parasitic intestinal infections.

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Amino Acid and Protein Metabolism

North

Lockwood

1995

Biochemistry and Molecular Biology of Parasites

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Amino acid catabolism in the nematodesHeligmosomoides polygyrusandPanagrellus redivivus2. Metabolism of the carbon skeleton

Cited by 7 publications

References 22 publications

NAD(P)H fluorescence lifetime imaging of live intestinal nematodes reveals metabolic crosstalk between parasite and host

NAD(P)H fluorescence lifetime imaging of live intestinal nematodes reveals metabolic crosstalk between parasite and host

NAD(P)H Fluorescence Lifetime Imaging of Live Intestinal Nematodes Reveals Metabolic Crosstalk Between Parasite and Host

Amino Acid and Protein Metabolism

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