Amino Acid Changes in Pepper mild mottle virus Coat Protein That Affect L 3 Gene-mediated Resistance in Pepper

Hamada, Hiroyuki; Takeuchi, Shigeharu; Kiba, Akinori; Tsuda, Shinya; Hikichi, Yasufumi; Okuno, Tetsuro

doi:10.1007/pl00013069

Cited by 40 publications

(57 citation statements)

References 16 publications

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“…L 3 -genemediated resistance is elicited by the coat protein (CP) of tobamoviruses (Berzal-Herranz et al 1995;Gilardi et al 2004). Analyses of chimeric and point-mutated viruses have indicated that various single or double amino acid changes in the PMMoV CP can overcome L 3 -gene-mediated resistance (Berzal-Herranz et al 1995;Hamada et al 2002;Tsuda et al 1998). In our previous study, both the spatial and temporal patterns of defense-related gene induction were critical factors in restricting virus spread by L 3 -gene-mediated resistance (Hamada et al 2005), suggesting that the extent of the early response to the viral elicitor is important in determining the overall host response.…”

Section: Introductionmentioning

confidence: 98%

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SuperSAGE revealed different classes of early resistance response genes in Capsicum chinense plants harboring L 3 -resistance gene infected with Pepper mild mottle virus

et al. 2008

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We used SuperSAGE, an improved version of serial analysis of gene expression, to explore transcriptome changes early in the L 3 -mediated resistance response of pepper plants against a tobamovirus. Capsicum chinense plants homozygous for the L 3 resistance gene were infected with virulent and avirulent strains of Pepper mild mottle virus (PMMoV). Plants were maintained at a temperature nonpermissive for the resistance gene to allow the viruses to spread, then transferred to a permissive temperature for 3 h and subsequently analyzed. In the incompatible reaction, we selected 152 SuperSAGE tags (each 26 nucleotides long) possibly corresponding to upregulated genes, and 84 tags for downregulated genes. Approximately 70% of tags had matching ESTs in the genus Capsicum, other genera within the Solanaceae and/or other families of plants. More than 90% of tags with EST matches could be annotated with either functionally characterized or uncharacterized proteins. We compared genes annotated by SuperSAGE tags and those annotated by partial cDNA that was obtained using the SuperSAGE tag sequences as rapid amplification of the cDNA ends-PCR primers. Of genes annotated by SuperSAGE tags, c. 90% were consistent with those annotated by longer cDNA sequences. We cloned 17 full-length cDNAs from different SuperSAGE tags and confirmed that these genes were upregulated during normal infection in the incompatible interaction. We identified several early resistance response genes including a Ran/TC4 protein and a b-oxidation multifunctional protein, indicating that SuperSAGE is a powerful tool for investigating plant-pathogen interactions.

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Section: Introductionmentioning

confidence: 98%

“…A system meeting these requirements is comprised of Pepper mild mottle virus (PMMoV) isolates and Capsicum plants harboring the tobamovirus resistance gene, L 3 (Berzal-Herranz et al 1995;García-Luque et al 1993;Hamada et al 2002;Tsuda et al 1998). …”

Section: Introductionmentioning

confidence: 99%

SuperSAGE revealed different classes of early resistance response genes in Capsicum chinense plants harboring L 3 -resistance gene infected with Pepper mild mottle virus

et al. 2008

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“…A troca de uma metionina (M) por asparagina (N) determina o fenótipo de quebra de resistência do gene L3, representando o aminoácido-chave para tal evento (Velasco et al, 2002). Estes aspectos, relativos à seqüência da CP têm sido, recentemente, investigados por outros autores como diretamente ligados à resistência, sendo a proteína da capa considerada como elicitor de resposta do hospedeiro à infecção (Berzal-Herranz et al, 1995;Tsuda et al, 1998;Hamada et al, 2002). A análise filogenética das seqüências da CP de isolados do PMMoV permitiu a sua separação em dois grupos distintos denominados de: grupo "L3-resistance" -patótipos P12, que engloba o isolado brasileiro (PMMoV-BR) e os demais isolados que não são capazes de quebrar a resistência do gene L3; e o grupo "L3 resistance overcoming" -patótipos P123, que engloba o isolado italiano (PMMoV-Italian), espanhol (PMMoV-Ia) e um isolado japonês (PMMoV-Tosa), os quais promovem a quebra da resistência do gene L3 (Figura 3).…”

Section: Do Gênerounclassified

“…pelos patótipos P123 tem sido objeto de estudo e preocupação porque tem levado a perdas importantes em cultivos de pimenta e pimentão na Europa (Alonso et al, 1991;Letschert et al, 2002;Velasco et al, 2002). Na árvore filogenética nota-se também o isolado PMMoV-Tosa, proveniente do Japão, apresentando 100% de identidade com o isolado PMMoVItalian, podendo ter sido introduzido da Itália por intercâmbio de material vegetal contaminado (Hamada et al, 2002).…”

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Caracterização de um isolado do Pepper mild mottle virus que não quebra a resistência do gene L3 em Capsicum sp.

et al. 2004

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RESUMO ABSTRACT Characterization of a non-L3 gene-resistance breaking Pepper mild mottle virus isolate in CapsicumA strain of Pepper mild mottle virus (PMMoV) was identified by biological and serological analysis of pepper (Capsicum baccatum) 'Dedo de Moça' seeds from São Paulo, SP, Brazil. To confirm these results, RT-PCR was performed with specific primers flanking the coat protein (CP) ORF of tobamoviruses in subgroup 1. The DNA-amplified fragments, when sequenced and compared with other tobamovirus CP sequences stored in the GenBank, showed nucleotide similarity between 94 to 100% with other PMMoV CP sequences, and below 75% with the subgroup I tobamovirus species (Tobacco mosaic virus, Tomato mosaic virus and Odontoglossum ringspot virus) and 65% with species in the subgroups II and III. The Brazilian isolate showed 100% identity with a Japanese isolate, strongly suggesting that this pathogen could have been introduced from that country. This fact confirms the importance of the isolate's characterization, in order to establish adequate control measures to guarantee healthy seed interchange and to avoid the introduction and spread of tobamoviruses into new growing areas. The deduced CP aminoacids sequence showed that the PMMoV-BR was not capable to overcome L3 gene resistance. This fact was confirmed by the inoculation and reaction of differential Capsicum species. The Brazilian isolate was not able to infect C. chinense (L3) and C. chacoense (L4). Do gênero

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“…Tobamoviruses infecting Capsicum plants are classified into four pathotypes, P 0 , P 1 , P 1,2 , and P 1,2,3 , based on the infection patterns in Capsicum plants carrying the different L genes (Rast 1988). The coat protein (CP) of tobamoviruses has been shown to elicit L 2 -or L 3 -genemediated resistance (Berzal-Herranz et al 1995;de la Cruz et al 1997), and amino acid changes in the CP responsible for overcoming L 3 -gene-mediated resistance have been identified (Berzal-Herranz et al 1995;Hamada et al 2002;Tsuda et al 1998).…”

Section: Introductionmentioning

confidence: 98%

Discrimination between tobamoviruses and their pathotypes for L-gene-mediated resistance in green pepper (Capsicum annuum L.) by reverse transcription-polymerase chain reaction

Takeuchi¹,

Hamada

Toyoda

et al. 2005

J Gen Plant Pathol

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A new method to discriminate between tobamoviruses and their pathotypes that infect green pepper (Capsicum annuum L.) was developed using reverse transcription-polymerase chain reaction (RT-PCR). The P 0 pathotype (Tobacco mosaic virus, Tomato mosaic virus, and Tobacco mild green mosaic virus) and the P 1 pathotype (Paprika mild mottle virus) were distinguished by RT-PCR using primers specific to each pathotype. However, the P 1,2 and P 1,2,3 pathotypes of Pepper mild mottle virus (PMMoV) could not be distinguished from each other using this procedure. The P 1,2 and P 1,2,3 pathotypes were differentiated by RT-nested PCR, in which a DNA fragment was first produced by RT-PCR using primers containing conserved sequences of PMMoV. The product was then used as the DNA template in a second PCR using primers specific to each pathotype. An immunocapture (IC) RT-PCR method was developed based on results from this study, which facilitated detection of tobamoviruses in pepper plants, seeds, and field soils and allowed the identification of their pathotypes.

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Amino Acid Changes in Pepper mild mottle virus Coat Protein That Affect L 3 Gene-mediated Resistance in Pepper

Cited by 40 publications

References 16 publications

SuperSAGE revealed different classes of early resistance response genes in Capsicum chinense plants harboring L 3 -resistance gene infected with Pepper mild mottle virus

SuperSAGE revealed different classes of early resistance response genes in Capsicum chinense plants harboring L 3 -resistance gene infected with Pepper mild mottle virus

Caracterização de um isolado do Pepper mild mottle virus que não quebra a resistência do gene L3 em Capsicum sp.

Discrimination between tobamoviruses and their pathotypes for L-gene-mediated resistance in green pepper (Capsicum annuum L.) by reverse transcription-polymerase chain reaction

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