Amino acid reference intervals by high performance liquid chromatography in plasma sample of Brazilian children

Cruz, Ana F.; Barbosa, Tatiana Maria C. C.; Adelino, Talita; Lima, William P.; Mendes, Magda O.; Valadares, Eugênia Ribeiro

doi:10.5935/1676-2444.20160012

Cited by 4 publications

(6 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Authors such as Sharma et al (2014), Wada et al (2014) and Cruz et al (2016) presented a similar determination coefficient to that found in this study ( R 2 ˃ 0.99). In cases where we obtained the coefficient of determination <0.99, it was observed that they were random results and in only one of the three replicates performed.…”

Section: Discussionsupporting

confidence: 88%

“…Thus, the final product generated (derivatized amino acids) becomes quite stable and easy to store for up to one week at a temperature of 2–8°C. According to Feeks (1996) and Cruz et al (2016), other derivatization agents such as ortho ‐phthalaldehyde leaves the final product unstable and can be rapidly converted into nonfluorescent products.…”

Section: Discussionmentioning

confidence: 99%

“…In other studies described in the literature, such as Shawky, Abd‐Elkhalek, and Elakhadar (2015), Cruz et al (2016) and Strauss et al (2010), the same number of patients was used to determine the reference value for amino acids in urine and blood on filter paper.…”

Section: Discussionmentioning

confidence: 99%

“…Establishing the reference value for each laboratory is the most appropriate way to optimize use in routine laboratories and avoid incorrect diagnosis (Cruz et al, 2016; McPherson & Pincus, 2017; Ozarda, 2016). After analyzing 58 blood samples and urine samples collected on filter paper from participants in this study, standardized reference values are described in Table 4.…”

Section: Discussionmentioning

confidence: 99%

See 3 more Smart Citations

Quantitative analysis of amino acids by HPLC in dried blood and urine in the neonatal period: Establishment of reference values

Nolasco

Fortes

Valadares

2020

Biomedical Chromatography

Self Cite

View full text Add to dashboard Cite

Quantitative analysis of amino acids in blood and urine is primarily indicated for the diagnosis of amino acid disorders. The high-performance liquid chromatography (HPLC) technique is frequently used for this detection. The frequency of sample collection on filter paper has been increasing exponentially, and there are many advantages attributed to processing biological samples in this way. The aim of this study was to validate a quantitative analysis of amino acids by HPLC in blood and urine collected on filter paper and to establish reference values in the neonatal period. Dried blood and dried urine samples of respectively 58 and 45 healthy newborns (2-9 days) were collected. Pre-treatment and extraction of samples were done according to the literature. Separation and analysis of amino acids were carried out by HPLC with fluorescence detection. The developed method demonstrated excellent separation, linearity, limits of detection and quantification, repeatability and recovery. The reference values for 17 amino acids were defined in dried blood and urine samples of newborns. This work presents a simple, fast and effective method for the simultaneous analysis of 17 amino acids in blood and urine collected on filter paper in a single run. The reference values were established and validated. K E Y W O R D S aminoacidopathies, dried blood samples, dried urine samples, high-performance liquid chromatography, inborn errors of metabolism, newborn screening

show abstract

Section: Discussionsupporting

confidence: 88%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Quantitative analysis of amino acids by HPLC in dried blood and urine in the neonatal period: Establishment of reference values

Nolasco

Fortes

Valadares

2020

Biomedical Chromatography

Self Cite

View full text Add to dashboard Cite

show abstract

“…It seems that children under one year need specific reference value. Cruz et al in their study validated the HPLC method for determination plasma amino acids and established refer-ence interval for Brazilian population (30). They recommended that each laboratory should establish its own reference interval due to variation in methodology and analytical techniques used in published data.…”

Section: Discussionmentioning

confidence: 99%

Reference Interval for Plasma Free Amino Acids in Iranian Children Using Reverse Phase High Performance Liquid Chromatography

et al. 2018

View full text Add to dashboard Cite

Background: Brain damage and mental retardation in children can be caused by either excess or depletion of certain amino acids. Up to now there is no reliable reference interval for free plasma amino acid in Iranian children. Objectives: The aim of this study was to provide the reference intervals of free plasma amino acid in healthy Iranian children using high performance liquid chromatography (HPLC) as an accurate method. Methods: 133 children referred to Children's Medical Center, Tehran, Iran for the annual checkup entered the study after filling out the informed consent form. Demographic data and blood samples after overnight fasting were collected. Plasma amino acid concentrations were determined by reverse phase HPLC (RP-HPLC) equipped with a fluorescence detector after precolumn derivatization of amino acids with o-phthalaldehyde (OPA). Results: Female children exhibited significantly higher plasma concentrations of taurine and lysine than male children. Other 20 amino acid values were not significantly different between two gender groups. Positive correlation was obsereved between age and taurine, leucine and lysine. Conclusions: Our data provided reference intervals of free plasma amino acids in healthy Iranian children. We believe our data can be used as a guideline for pediatricians to identify the significantly deviated amino acid values in children with medical issues such as genetic disorders and some acquired illnesses. Our data is also applicable for further investigations in the field of family medicine.

show abstract

Age-Specific Cut-off Values of Amino Acids and Acylcarnitines for Diagnosis of Inborn Errors of Metabolism Using Liquid Chromatography Tandem Mass Spectrometry

Sarker

Islam

Biswas

et al. 2019

BioMed Research International

View full text Add to dashboard Cite

Liquid Chromatography tandem mass spectrometry (LC-MS/MS) is used for the diagnosis of more than 30 inborn errors of metabolisms (IEMs). Accurate and reliable diagnosis of IEMs by quantifying amino acids (AAs) and acylcarnitines (ACs) using LC-MS/MS systems depend on the establishment of age-specific cut-offs of the analytes. This study aimed to (1) determine the age-specific cut-off values of AAs and ACs in Bangladesh and (2) validate the LC-MS/MS method for diagnosis of the patients with IEMs. A total of 570 enrolled healthy participants were divided into 3 age groups, namely, (1) newborns (1-7 days), (2) 8 days–7 years, and (3) 8–17 years, to establish the age-specific cut-offs for AAs and ACs. Also, 273 suspected patients with IEMs were enrolled to evaluate the reliability of the established cut-off values. Quantitation of AAs and ACs was performed on an automated LC-MS/MS system using dried blood spot (DBS) cards. Then the specimens of the enrolled clinically suspected patients were analyzed by the established method. Nine patients came out as screening positive for different IEMs, including two borderline positive cases of medium-chain acyl-CoA dehydrogenase deficiency (MCAD). A second-tier test for confirmation of the screening positive cases was conducted by urinary metabolic profiling using gas chromatography- mass spectrometry (GC-MS). Out of 9 cases that came out as screening positive by LC-MS/MS, seven cases were confirmed by urinary GC-MS analysis including 3 cases with phenylketonuria, 1 with citrullinemia type II, 1 with methylmalonic acidemia, 1 with isovaleric acidemia and 1 with carnitine uptake defect. Two borderline positive cases with MCAD were found negative by urinary GC-MS analysis. In conclusion, along with establishment of a validated LC-MS/MS method for quantitation of AAs and ACs from the DBS cards, the study also demonstrates the presence of predominantly available IEMs in Bangladesh.

show abstract

Amino acid reference intervals by high performance liquid chromatography in plasma sample of Brazilian children

Cited by 4 publications

References 10 publications

Quantitative analysis of amino acids by HPLC in dried blood and urine in the neonatal period: Establishment of reference values

Quantitative analysis of amino acids by HPLC in dried blood and urine in the neonatal period: Establishment of reference values

Reference Interval for Plasma Free Amino Acids in Iranian Children Using Reverse Phase High Performance Liquid Chromatography

Age-Specific Cut-off Values of Amino Acids and Acylcarnitines for Diagnosis of Inborn Errors of Metabolism Using Liquid Chromatography Tandem Mass Spectrometry

Contact Info

Product

Resources

About