1992
DOI: 10.1016/0161-5890(92)90174-v
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Amino acid sequence requirements for the epitope recognized by a monoclonal antibody reacting with the secreted antigen GP28.5 of Toxoplasma gondii

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Cited by 10 publications
(4 citation statements)
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“…The minigene encoded six antigen segments of T. gondii (SAG1 [238][239][240][241][242][243][244][245][246][247][248][249][250][251][252][253][254][255][256] ), which contains many T-and B-cell epitopes (Kang et al 2000;Alix 2000;Godard et al 1994;VelgeRoussel et al 1994;Siachoque et al 2006;Duquesne et al Duquesne et al 1991;Beghetto et al 2001;Mévélec et al 1998;Meisel et al 1996;Chardès et al 1993;Murray et al 1993;Cesbron-Delauw et al 1992). To minimize interference between adjacent antigen segments, each segment was separated from its neighboring one by five glycine codons.…”
Section: Recombinant Plasmid Constructionmentioning
confidence: 97%
“…The minigene encoded six antigen segments of T. gondii (SAG1 [238][239][240][241][242][243][244][245][246][247][248][249][250][251][252][253][254][255][256] ), which contains many T-and B-cell epitopes (Kang et al 2000;Alix 2000;Godard et al 1994;VelgeRoussel et al 1994;Siachoque et al 2006;Duquesne et al Duquesne et al 1991;Beghetto et al 2001;Mévélec et al 1998;Meisel et al 1996;Chardès et al 1993;Murray et al 1993;Cesbron-Delauw et al 1992). To minimize interference between adjacent antigen segments, each segment was separated from its neighboring one by five glycine codons.…”
Section: Recombinant Plasmid Constructionmentioning
confidence: 97%
“…The following mAbs were used for western blotting, immunoaffinity purification of native proteins and microarray experiments: TG 17.179 anti-GRA2 (Charif et al 1990;Cesbron-Delauw et al 1992), T3 4A7 anti-ROP2 and anti-ROP4 Leriche and Dubremetz 1991) and T4 1E5 anti-SAG-1 (Couvreur et al 1988). …”
Section: Immunoblotting and Antibodiesmentioning
confidence: 99%
“…1B) as well as by antibodies specific of each GRA protein, i.e. the mAb TG17.179, which recognizes the 8 C-terminal amino acids of GRA2 [12] ( Fig. 1C) and the rabbit serum raised against a denatured rGRA6 [13] (Fig.…”
Section: Purification Of Recombinant Proteinsmentioning
confidence: 99%
“…The recombinant proteins were detected using the mouse monoclonal antibody (mAb) TG 17-179 which recognizes the 8 Cterminal a.a. of GRA2 [11,12] (Biotem, France), a rabbit serum directed against the full length denatured recombinant GRA6 (gift from L.D. Sibley) [13], or the mAb Ab-1 anti-Hisx6 (Oncogene; gift from M.A.…”
Section: Antibodiesmentioning
confidence: 99%