Amino acids analysis using a monolithic silica column after derivatization with 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F)

Song, Yanting; Funatsu, Takashi; Tsunoda, Makoto

doi:10.1016/j.jchromb.2010.12.018

Cited by 38 publications

(25 citation statements)

References 31 publications

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“…The LODs and LOQs of NBD-amino acids were 0.6-17 fmol/injection and 1.9-57 fmol/injection, respectively. These values are similar with those reported in previous studies with monolithic silica columns [10], particle packed columns [14], and core-shell particle columns [15].…”

Section: Validation Datasupporting

confidence: 92%

“…Sample derivatization was performed according to previous studies [10][11][12]. A volume of 20 μL of the sample was added to 180 μL of a 0.2 M borate buffer (pH 8.5).…”

Section: Fluorescence Derivatizationmentioning

confidence: 99%

“…Based on our previous study using a monolithic silica column (MonoClad C18-HS) [10], the separation of NBD-amino acids was first performed by using the mobile phases of the solvent mixture A, consisting of water/acetonitrile/TFA (90/10/0.12, v/v/v) and the solvent mixture B, comprised of water/acetonitrile/TFA (10/90/0.12, v/v/v) for gradient elution. Although the retention ability of MonoTower column was similar to that of the MonoClad column, the NBD-amino acids could not be separated well.…”

Section: Separation Of Nbd-amino Acidsmentioning

confidence: 99%

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Amino Acid Analysis Using a Cartridge-Type Monolithic Silica Column

2017

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In this study, the application of a new type of monolithic silica column to the analysis of amino acids in human plasma samples has been investigated. The monolithic silica column (3.0 × 150 mm, MonoTower C18) provided good separation of the amino acids derivatized with 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F). Although the separation ability of this column was not much improved in comparison with the previous monolithic silica columns, it was similar with that of a 3 μm particle packed column. The column with length 150 mm had the same separation property as a tandem connection of columns of lengths 50 mm and 100 mm. After 1000 injections of human plasma samples, the peak widths for the NBD-amino acids were increased by only 8%. Thus, the newly developed monolithic silica column can be potentially applied for faster analysis and/or more efficient separation of biological compounds.

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Section: Validation Datasupporting

confidence: 92%

“…Sample derivatization was performed according to previous studies [10][11][12]. A volume of 20 μL of the sample was added to 180 μL of a 0.2 M borate buffer (pH 8.5).…”

Section: Fluorescence Derivatizationmentioning

confidence: 99%

Section: Separation Of Nbd-amino Acidsmentioning

confidence: 99%

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Amino Acid Analysis Using a Cartridge-Type Monolithic Silica Column

2017

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“…The derivatization procedure of the aliphatic amines was described previously [25][26][27]. Briefly, 20 µL of the aliphatic amine solution and 40 µL of 10 mM the NBD-F solution were mixed with 180 µL of a 0.2 M borate buffer (pH 8.5).…”

Section: Derivatization Procedures Of Aliphatic Aminesmentioning

confidence: 99%

Retention and Bandwidth Predictions by Fast Gradient Elution Chromatography Using a Pillar Array Column

et al. 2016

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Previously, we have developed a gradient elution system for pillar array columns, which achieved faster separation than isocratic elution. In this study, we validated gradient elution in microchip liquid chromatography (LC) and investigated the retention and bandwidth predictions of fluorescently labeled aliphatic amines in fast gradient elution chromatography using semi-empirical retention models. The retention times and peak widths under different gradient elution programs were predicted by three solvent strength models and compared with the experimental results. The relative errors of prediction for the retention times and peak widths were below 14 % and 12 %, respectively. The results showed that the solvent strength models could be utilized for predicting the retention times and peak widths under gradient elution in the microchip LC system and that the gradient elution program for pillar array columns worked efficiently. The prediction by the retention model promises to be a potential tool for essential compound identification in biological samples.

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“…9,[13][14][15][16][17][18][19] In addition, 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F), which also reacts with amines, phenols, and water, has been used in aqueous solution at neutral and basic pH values, and the derivatization reaction can be terminated by the addition of an acid. 20,21 However, as these derivatization methods are not only applicable to aromatic amines, but also to phenols and aliphatic amines, a sample containing an interfering substance must first be cleaned up or pretreated by, for example, solid-phase extraction or liquid-liquid extraction methods. However, due to the requirements for analyzing large numbers of samples within a restricted time frame and budget, a selective derivatization for a simple and rapid method without pretreatment is desirable.…”

Section: Introductionmentioning

confidence: 99%

Selective Detection of Carcinogenic Aromatic Diamines in Aqueous Solutions Using 4-(N-Chloroformylmethyl-N-methylamino)-7-nitro- 2,1,3-benzoxadiazole (NBD-COCl) by HPLC

Inoue

2017

ANAL. SCI.

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Reaction conditions for the selective derivatization of three types of aromatic diamines without clean-up in an aqueous solution using 4-(N-chloroformylmethyl-N-methylamino)-7-nitro-2,1,3-benzoxadiazole (NBD-COCl) have been developed for simple, fast, and selective analysis by HPLC. The aromatic amines were derivatized to give di-NBD-CO-aromatic amines at 35 C for 5 min in a buffer solution at pH 5 containing a low acetonitrile concentration (0 -400 ng/mL, n = 5, r >0.998). The retention times of these di-NBD-CO-aromatic amines were <5 min. Sample solutions containing aromatic amines, phenols, and an aliphatic amine were also prepared. Under the optimized reaction conditions (i.e., low acetonitrile content and acidic conditions), no derivatization of the phenols and the aliphatic amine was observed. These were related to the differences in the pKa values of the target substances, the organic solvent concentration, and the reaction solution pH. For the model system, a sulfuric acid-impregnated filter was spiked with the aromatic amines, phenols, and aliphatic amine prior to extraction, derivatization, and measurement by HPLC. Only aromatic amines were detected quantitatively, with no other compounds being observed.

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Amino acids analysis using a monolithic silica column after derivatization with 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F)

Cited by 38 publications

References 31 publications

Amino Acid Analysis Using a Cartridge-Type Monolithic Silica Column

Amino Acid Analysis Using a Cartridge-Type Monolithic Silica Column

Retention and Bandwidth Predictions by Fast Gradient Elution Chromatography Using a Pillar Array Column

Selective Detection of Carcinogenic Aromatic Diamines in Aqueous Solutions Using 4-(N-Chloroformylmethyl-N-methylamino)-7-nitro- 2,1,3-benzoxadiazole (NBD-COCl) by HPLC

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