Amino Acids: Metabolism

Otašević, Vesna; Korać, Bato

doi:10.1016/b978-0-12-384947-2.00028-3

Cited by 9 publications

(7 citation statements)

References 10 publications

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“…One approach for improving protein efficiency of dietary formulations is to mimic the proportional composition of amino acids contained in the muscle tissue to assure the presence of all essential amino acids [3]. However, to meet the exact requirements to generate data that can potentially be use to the formulation of efficient feeds, knowledge of the bioavailability of each dietary amino acid to be absorbed and retained is needed [4]. The bioavailability could be indirectly estimated through the determination of the apparent digestibility of the dietary essential amino acids.…”

Section: Introductionmentioning

confidence: 99%

Metabolism of Seriola lalandi during Starvation as Revealed by Fatty Acid Analysis and Compound-Specific Analysis of Stable Isotopes within Amino Acids

Barreto-Curiel¹,

Focken

D’Abramo

et al. 2017

PLoS ONE

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Fish starvation is defined as food deprivation for a long period of time, such that physiological processes become confined to basal metabolism. Starvation provides insights in physiological processes without interference from unknown factors in digestion and nutrient absorption occurring in fed state. Juveniles of amberjack Seriola lalandi were isotopically equilibrated to a formulated diet for 60 days. One treatment consisted of fish that continued to be fed and fish in the other treatment were not fed for 35 days. The isotopic signatures prior to the beginning of and after the starvation period, for fish in the starvation and control treatments, were analysed for lipid content, fatty acid composition and isotopic analysis of bulk (EA-IRMS) and of amino acids (compound specific isotope analysis, CSIA). There were three replicates for the starvation group. Fatty acid content in muscle and liver tissue before and after starvation was determined to calculate percent change. Results showed that crude lipid was the most used source of energy in most cases; the PUFAs and LC-PUFAs were highly conserved. According to the protein signature in bulk (δ15N) and per amino acid (δ13C and δ15N), in muscle tissue, protein synthesis did not appear to occur substantially during starvation, whereas in liver, increases in δ13C and δ15N indicate that protein turnover occurred, probably for metabolic routing to energy-yielding processes. As a result, isotopic values of δ15N in muscle tissue do not change, whereas CSIA net change occurred in the liver tissue. During the study period of 35 days, muscle protein was largely conserved, being neither replenished from amino acid pools in the plasma and liver nor catabolized.

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Section: Introductionmentioning

confidence: 99%

Metabolism of Seriola lalandi during Starvation as Revealed by Fatty Acid Analysis and Compound-Specific Analysis of Stable Isotopes within Amino Acids

Barreto-Curiel¹,

Focken

D’Abramo

et al. 2017

PLoS ONE

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“…It has been known that amino acids are important biomolecules that both serve as building blocks of proteins and intermediates in various metabolic pathways and also act as regulators of gene expression and protein phosphorylation cascade. Furthermore, their metabolic role is tightly coupled with the metabolism of glucose and fatty acids . Metabolic pathway analysis results showed that the amino acid metabolism was disturbed in patients with EEDA.…”

Section: Resultsmentioning

confidence: 99%

“…Furthermore, their metabolic role is tightly coupled with the metabolism of glucose and fatty acids. 19 Metabolic pathway analysis results showed that the amino acid metabolism was disturbed in patients with EEDA.…”

Section: Pathway Analysis Of Differential Metabolitesmentioning

confidence: 99%

Metabolomics Identifies a Panel of Diagnostic Biomarkers for Early Human Embryonic Development Arrest

et al. 2023

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Early embryonic development arrest (EEDA) is a unique form of early spontaneous abortion in pregnant women, which is previously suggested to be associated with metabolic abnormalities. Noninvasive biomarkers would significantly improve its diagnosis and clinical outcome. Here, we performed a targeted metabolomics study in plasma from EEDA patients (n = 27) and normal pregnant women (NPW, n = 27) using liquid chromatography coupled with mass spectrometry (LC–MS) to identify potential diagnostic marker metabolites. Our results showed significantly different plasma metabolic profiles between EEDA patients and NPW. Particularly, EEDA patients showed significant alterations in amino acid, carbohydrate, and vitamin metabolism, which were characterized by 21 significantly increased metabolites and five decreased metabolites in plasma. Further receiver operating characteristic analysis showed that an optimal combination of S-methyl-5′-thioadenosine, kynurenine, leucine, and malate could be used as a panel of metabolites for EEDA diagnosis. The area under the curve of the metabolite panel was 0.941, suggesting a better performance than any single metabolite for the diagnosis of EEDA. In summary, our study identifies a panel of differential metabolites in plasma that could act as potential biomarkers for the diagnosis of EEDA in clinical settings.

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“…However, in birds challenged with Eimeria , the induced oxidative stress and inflammation could potentially amplify the adverse consequences of Hcy accumulation, thereby further compromising bone health. Known that Cys also possesses potent antioxidant capacity without contributing to Hcy accumulation ( Atmaca, 2004 ; Otasevic and Korac, 2016 ). Partially substituting Met supplementation with Cys in diets of broilers challenged by Eimeria might potentially improve the bone health of the birds.…”

Section: Introductionmentioning

confidence: 99%

Different methionine to cysteine supplementation ratios altered bone quality of broilers with or without Eimeria challenge assessed by dual energy X-ray absorptiometry and microtomography

Liu,

Sharma,

Tompkins

et al. 2024

Poultry Science

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Amino Acids: Metabolism

Cited by 9 publications

References 10 publications

Metabolism of Seriola lalandi during Starvation as Revealed by Fatty Acid Analysis and Compound-Specific Analysis of Stable Isotopes within Amino Acids

Metabolism of Seriola lalandi during Starvation as Revealed by Fatty Acid Analysis and Compound-Specific Analysis of Stable Isotopes within Amino Acids

Metabolomics Identifies a Panel of Diagnostic Biomarkers for Early Human Embryonic Development Arrest

Different methionine to cysteine supplementation ratios altered bone quality of broilers with or without Eimeria challenge assessed by dual energy X-ray absorptiometry and microtomography

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