Amitraz and its metabolite differentially activate <i>α</i>‐ and <i>β</i>‐adrenergic‐like octopamine receptors

Kita, Tomo; Hayashi, Takeshi; Ohtani, Tomohiro; Takao, Haruka; Takasu, Hiroshi; Liu, Genyan; Ohta, Hiroto; Ozoe, Fumiyo; Ozoe, Yoshihisa

doi:10.1002/ps.4412

Cited by 62 publications

(47 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…). It is believed that NlOARs play a critical role in the central nervous system and the high enrichment of these NlOARs within the central nervous system has been beneficial for the exploitation of insecticides targeting these receptors . In Malpighian tubules, an important tissue for urine production and ion exchange, NlOA1 was the most highly expressed gene compared with others.…”

Section: Resultsmentioning

confidence: 99%

Molecular features and expression profiles of octopamine receptors in the brown planthopper, Nilaparvata lugens

Huang

et al. 2019

Pest Management Science

View full text Add to dashboard Cite

BACKGROUND Octopamine, the invertebrate counterpart of adrenaline and noradrenaline, regulates and modulates many physiological and behavioral processes in insects. It mediates its effects by binding to specific octopamine receptors, which belong to the superfamily of G‐protein coupled receptors (GPCRs). The expression profiles of octopamine receptor genes have been well documented in different developmental stages and multiple tissue types in several different insect orders. However, little work has addressed this issue in Hemiptera. RESULTS In this study, we cloned four octopamine receptor genes from brown planthopper. The deduced amino acid sequences share high identity with other insect homologues and have the characteristic GPCRs domain architecture: seven transmembrane domains. These genes were expressed in all developmental stages and examined tissues. The expression of NlOA2B3 and NlOA3 was relatively higher in egg and first instar nymph stage than in other stages and other receptor genes. All of these receptor genes were more highly expressed in brain than other tissues. CONCLUSION The identification of octopamine receptor genes in this study will provide a foundation for investigating the diverse roles played by NlOARs and for exploring specific target sites for chemicals that control agricultural pests. © 2019 Society of Chemical Industry

show abstract

Section: Resultsmentioning

confidence: 99%

Molecular features and expression profiles of octopamine receptors in the brown planthopper, Nilaparvata lugens

Huang

et al. 2019

Pest Management Science

View full text Add to dashboard Cite

show abstract

“…This provides a structural and functional basis for the development of specific insecticides. The β‐OAR is an important molecular target for formamidine insecticides (Kita et al., ). For example, amitraz is a formamidine acaricide insecticide used to control ticks, mites, and fleas.…”

Section: Discussionmentioning

confidence: 99%

“…At amitraz concentrations of 1, 10, and 100 μM, intracellular cAMP concentrations were 1.75, 6.35, and 20.5 times higher than in controls. Furthermore, both amitraz and its metabolite N2‐(2,4‐dimethylphenyl)‐N1‐methyformamidine (DPMF) acted as OAR agonists, and the metabolite DPMF was more potent than amitraz‐activated β‐adrenergic‐like OARs (Kita et al., ). We speculate that the weak affinity of amitraz for the β‐OAR might be one reason that amitraz could not be used to control Lepidoptera pests.…”

Section: Discussionmentioning

confidence: 99%

“…Octopamine receptors (OARs) are the typical GPCRs found in invertebrate species. Homologous to the adrenaline receptor family in vertebrates (Evans & Maqueira, ), OARs represent potential targets for newer and safer insecticides (Kita et al., ). According to their sequence homology, OARs can be divided into two categories: α‐OARs (orthologous to α‐adrenergic receptors) and β‐OARs (orthologous to β‐adrenergic receptors).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Pharmacological characterization of a β‐adrenergic‐like octopamine receptor in Plutella xylostella

Huang

Deng

et al. 2018

Arch Insect Biochem Physiol

View full text Add to dashboard Cite

The β-adrenergic-like octopamine receptor (OA2B2) belongs to the class of G-protein coupled receptors. It regulates important physiological functions in insects, thus is potentially a good target for insecticides. In this study, the putative open reading frame sequence of the Pxoa2b2 gene in Plutella xylostella was cloned. Orthologous sequence alignment, phylogenetic tree analysis, and protein sequence analysis all showed that the cloned receptor belongs to the OA2B2 protein family. PxOA2B2 was transiently expressed in HEK-293 cells. It was found that PxOA2B2 could be activated by both octopamine and tyramine, resulting in increased intracellular cyclic AMP (cAMP) levels, whereas dopamine and serotonin were not effective in eliciting cAMP production. Further studies with series of PxOA2B2 agonists and antagonists showed that all four tested agonists (e.g., naphazoline, clonidine, 2-phenylethylamine, and amitraz) could activate the PxOA2B2 receptor, and two of tested antagonists (e.g., phentolamine and mianserin) had significant antagonistic effects. However, antagonist of yohimbine had no effects. Quantitative real-time polymerase chain reaction analysis showed that Pxoa2b2 gene was expressed in all developmental stages of P. xylostella and that the highest expression occurred in male adults. Further analysis with fourth-instar P. xylostella larvae showed that the Pxoa2b2 gene was mainly expressed in Malpighian tubule, epidermal, and head tissues. This study provides both a pharmacological characterization and the gene expression patterns of the OA2B2 in P. xylostella, facilitating further research for insecticides using PxOA2B2 as a target.

show abstract

“…Octopamine and tyramine receptors are types of Gprotein coupled receptors that increase intracellular Ca 2+ concentrations and/or activate molecular signaling cascades [54]. These receptors are the target sites for formamidine insecticides, such as amitraz that is used as a miticide to control Varroa mites in honey bee colonies [55,56]. Mutations in these receptors may underlie amitraz resistance [57,58].…”

Section: Introductionmentioning

confidence: 99%

In silico identification and assessment of insecticide target sites in the genome of the small hive beetle, Aethina tumida

Rinkevich

Bourgeois

2020

BMC Genomics

View full text Add to dashboard Cite

Background: The small hive beetle, Aethina tumida, is a rapidly emerging global pest of honey bee colonies. Small hive beetle infestation can be extremely destructive, which may cause honey bees to abscond and render colony infrastructure unusable. Due to the impacts small hive beetles have on honey bees, a wide variety of physical, cultural, and chemical control measures have been implemented to manage small hive beetle infestations. The use of insecticides to control small hive beetle populations is an emerging management tactic. Currently, very little genomic information exists on insecticide target sites in the small hive beetle. Therefore, the objective of this study is to utilize focused in silico comparative genomics approaches to identify and assess the potential insecticide sensitivity of the major insecticide target sites in the small hive beetle genome. Results: No previously described resistance mutations were identified in any orthologs of insecticide target sites. Alternative exon use and A-to-I RNA editing were absent in AtumSC1. The ryanodine receptor in small hive beetle (Atum_Ryr) was highly conserved and no previously described resistance mutations were identified. A total of 12 nAChR subunits were identified with similar alternative exon use in other insects. Alternative exon use and critical structural features of the GABA-gated chloride channel subunits (Atum_RDL, Atum_GRD, and Atum_LCCH3) were conserved. Five splice variants were found for the glutamate-gated chloride channel subunit. Exon 3c of Atum_ GluCl may be a beetle-specific alternative exon. The co-occurrence of exons 9a and 9b in the pH-sensitive chloride channel (Atum_pHCl) is a unique combination that introduces sites of post-translational modification. The repertoire and alternative exon use for histamine-gated chloride channels (Atum-HisCl), octopamine (Atum_OctR) and tyramine receptors (Atum_TAR) were conserved. Conclusions: The recently published small hive beetle genome likely serves as a reference for insecticidesusceptible versions of insecticide target sites. These comparative in silico studies are the first step in discovering targets that can be exploited for small hive beetle-specific control as well as tracking changes in the frequency of resistance alleles as part of a resistance monitoring program. Comparative toxicity alongside honey bees is required to verify these in silico predictions.

show abstract

Amitraz and its metabolite differentially activate α‐ and β‐adrenergic‐like octopamine receptors

Cited by 62 publications

References 50 publications

Molecular features and expression profiles of octopamine receptors in the brown planthopper, Nilaparvata lugens

Molecular features and expression profiles of octopamine receptors in the brown planthopper, Nilaparvata lugens

Pharmacological characterization of a β‐adrenergic‐like octopamine receptor in Plutella xylostella

In silico identification and assessment of insecticide target sites in the genome of the small hive beetle, Aethina tumida

Contact Info

Product

Resources

About