Ammonia borane positively regulates cold tolerance in Brassica napus via hydrogen sulfide signaling

Abstract

Background Cold stress adversely influences rapeseeds (Brassica napus L.) growth and yield during winter and spring seasons. Hydrogen (H2) is a potential gasotransmitter that is used to enhance tolerance against abiotic stress, including cold stress. However, convenience and stability are two crucial limiting factors upon the application of H2 in field agriculture. To explore the application of H2 in field, here we evaluated the role of ammonia borane (AB), a new candidate for a H2 donor produced by industrial chemical production, in plant cold tolerance. Results The application with AB could obviously alleviate the inhibition of rapeseed seedling growth and reduce the oxidative damage caused by cold stress. The above physiological process was closely related to the increased antioxidant enzyme system and reestablished redox homeostasis. Importantly, cold stress-triggered endogenous H2S biosynthesis was further stimulated by AB addition. The removal or inhibition of H2S synthesis significantly abolished plant tolerance against cold stress elicited by AB. Further field experiments demonstrated that the phenotypic and physiological performances of rapeseed plants after challenged with cold stress in the winter and early spring seasons were significantly improved by administration with AB. Particularly, the most studied cold-stress response pathway, the ICE1-CBF-COR transcriptional cascade, was significantly up-regulated either. Conclusion Overall, this study clearly observed the evidence that AB-increased tolerance against cold stress could be suitable for using in field agriculture by stimulation of H2S signaling.

Differential Regulations of Antioxidant Metabolism and Cold-Responsive Genes in Three Bermudagrass Genotypes under Chilling and Freezing Stress

“…H 2 S activated the expression of chilling-responsive genes such as CBF3 , COR15A , and COR15B in favor of chilling tolerance in Arabidopsis thaliana [ 45 ]. Similarly, exogenous ammonia borane effectively alleviated CS damage to rapeseed ( Brassica napus ) seedlings through the H 2 S-regulated antioxidant system and CBF-COR pathway [ 46 ]. H 2 S-indcued increases in ROS-scavenging enzyme activities including, SOD, CAT, and APX, were propitious to mitigate ROS injury to hawthorn ( Crataegus monogyna ) fruit under CS [ 47 ].…”

Construction and Identification of Cold Tolerance in Different Broccoli Cultivars at the Seedling Stage

“…However, the frequent occurrence of extreme weather, high temperature, drought, cold damage, freezing damage, and other abiotic stresses seriously affect the harvest of broccoli [4]. Temperature directly affects the growth process, final yield, and flower quality of broccoli.…”

Role of tillage measures in mitigating waterlogging damage in rapeseed

“…The seedling stage of rapeseed is an important period for the population, which is dominated by vegetative growth. There is a close relationship between plant growth and rapeseed resistance and late yield [ 4 ]. Previous studies have shown that of rapeseed is an oil crop sensitive to waterlogging stress, especially during critical growth periods (e.g.…”