Ammonia stress affects the structure and function of hemocyanin in Penaeus vannamei

Zhao, Mintao; Aweya, Jude Juventus; Feng, Qian; Zheng, Zhihong; Yao, Defu; Zhao, Yang; Chen, Xiuli; Zhang, Yueling

doi:10.1016/j.ecoenv.2022.113827

Cited by 10 publications

(3 citation statements)

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“…[ 44 ]. In penaeid shrimp, the hepatopancreas expresses the highest mRNA transcripts of hemocyanin [ 43 , 45 ], which could be induced by environmental stress factors [ 35 , 39 ] and pathogens [ 46 ]. Here, we find that bacteria pathogens induced high levels of hemocyanin in shrimp hepatopancreas, coupled with a concomitant increase in the mRNA transcripts and protein levels of SREBP and key ER stress-related proteins ( Figure 1 ).…”

Section: Discussionmentioning

confidence: 99%

“…Most importantly, Pv SREBP could regulate the expression of the multifunctional respiratory protein hemocyanin [ 32 ] to affect the expression of other lipogenic and immune-related genes through a putative positive feedback mechanism [ 30 ]. Besides the role of hemocyanin in respiration and immune response [ 32 , 33 , 34 ], it plays a vital role in shrimp response to environmental cues, including hypoxia, pathogen stress, oxidative stress, ammonia stress, and thermal stress [ 35 , 36 , 37 , 38 , 39 , 40 ]. Given that hemocyanin is involved in shrimp response to various stressors and our recent finding that a putative positive feedback relationship exists between hemocyanin and SREBP, we wondered whether hemocyanin modulates ER stress to affect SREBP expression and fatty acid levels in penaeid shrimp.…”

Section: Introductionmentioning

confidence: 99%

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Modulation of SREBP Expression and Fatty Acid Levels by Bacteria-Induced ER Stress Is Mediated by Hemocyanin in Penaeid Shrimp

et al. 2023

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Many environmental and pathogenic insults induce endoplasmic reticulum (ER) stress in animals, especially in aquatic ecosystems, where these factors are crucial for life. In penaeid shrimp, pathogens and environmental stressors induce hemocyanin expression, but the involvement of hemocyanin in ER stress response is unknown. We demonstrate that in response to pathogenic bacteria (Vibrio parahaemolyticus and Streptococcus iniae), hemocyanin, ER stress proteins (Bip, Xbp1s, and Chop), and sterol regulatory element binding protein (SREBP) are induced to alter fatty acid levels in Penaeus vannamei. Interestingly, hemocyanin interacts with ER stress proteins to modulate SREBP expression, while ER stress inhibition with 4-Phenylbutyric acid or hemocyanin knockdown attenuates the expression of ER stress proteins, SREBP, and fatty acid levels. Contrarily, hemocyanin knockdown followed by tunicamycin treatment (ER stress activator) increased their expression. Thus, hemocyanin mediates ER stress during pathogen challenge, which consequently modulates SREBP to regulate the expression of downstream lipogenic genes and fatty acid levels. Our findings reveal a novel mechanism employed by penaeid shrimp to counteract pathogen-induced ER stress.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Modulation of SREBP Expression and Fatty Acid Levels by Bacteria-Induced ER Stress Is Mediated by Hemocyanin in Penaeid Shrimp

et al. 2023

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“…Similar to molluscs, arthropods are evolutionally distant from mammals (12), suggesting that hemocyanin from arthropods may induce a strong immune response in mammals. The hemocyanin from the white-leg shrimp ( Penaeus vannamei (P. vannamei) ) (13)(14), which belongs to the phylum Arthropoda, was considered after several logical thoughts. Firstly, P. vannamei is a traditional marine food and its aquaculture is prevalent worldwide.…”

Section: Introductionmentioning

confidence: 99%

Shrimp Hemocyanin elicits a potent humoral response in mammals and is favorable to hapten conjugation

Sun,

Wei,

Guo

et al. 2024

Preprint

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Conjugation to a carrier protein is essential to give rise to the antigenicity of hapten, which is a small molecule and cannot induce an immune response by itself alone. Three carrier proteins e.g. KLH (Keyhole Limpet Hemocyanin), BSA (Bovine Serum Albumin ), and OVA (Ovalbumin) were used mostly. KLH is advantageous to the others, majorly owing to its strong immunogenicity and limited usage in other biological assays. However, the solubility of KLH is not as well as the others, especially after hapten conjugation. Besides, the high market price of KLH results in high costs in vaccine and antibody development. Here, we extracted the shrimp hemocyanin (SHC) from Penaeus vannamei (P. vannamei) with a production yield of > 1 g proteins (98 % pure) per 1 kg shrimp. Compared to KLH, the peptide-SHC conjugates exhibit higher solubility after hapten conjugation. Furthermore, compared with KLH, SHC induces comparable antibody production efficiency in mammals, with or without conjugation. Finally, rabbit polyclonal antibodies or mouse monoclonal antibodies were generated by immunizing SHC-peptide conjugates, and their applications in western blot, immunofluorescence and immunohistochemistry were confirmed. Therefore, we demonstrated that SHC may be used as a substitute for KLH in future antibody and vaccine development

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The effects of non-lethal heat-shock-induced cross-protection on survival and growth of Pacific whiteleg shrimp, Litopenaeus vannamei in response to ionic stress in inland saline waters

et al. 2023

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Ammonia stress affects the structure and function of hemocyanin in Penaeus vannamei

Cited by 10 publications

References 78 publications

Modulation of SREBP Expression and Fatty Acid Levels by Bacteria-Induced ER Stress Is Mediated by Hemocyanin in Penaeid Shrimp

Modulation of SREBP Expression and Fatty Acid Levels by Bacteria-Induced ER Stress Is Mediated by Hemocyanin in Penaeid Shrimp

Shrimp Hemocyanin elicits a potent humoral response in mammals and is favorable to hapten conjugation

The effects of non-lethal heat-shock-induced cross-protection on survival and growth of Pacific whiteleg shrimp, Litopenaeus vannamei in response to ionic stress in inland saline waters

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