Ammonium removal at low temperature by a newly isolated heterotrophic nitrifying and aerobic denitrifying bacteriumPseudomonas fluorescenswsw-1001

Abstract

A heterotrophic nitrifier wsw-1001 was isolated from Songhua River and identified as Pseudomonas fluorescens. Ammonium removal by the strain at low temperature was investigated. The effect of initial ammonium concentration (from 5 to 1000 mg/L) and culture temperature (from 4°C to 30°C) on ammonium removal efficiency was studied. Biodegradation product, [Formula: see text], [Formula: see text], N2, N2O and intercellular N were monitored. The results indicated that the strain had potential for water and wastewater treatment. Ammonium could be removed by the strain at low temperature. Ammonium removal efficiency increased with temperature from 4°C to 20°C and decreased with ammonium concentration from 5 to 1000 mg/L. The strain exhibited a capability of heterotrophic nitrification and aerobic denitrification using [Formula: see text] as the sole nitrogen source at 8°C. [Formula: see text] and [Formula: see text] were reduced by the strain. Nitrogen balance analysis in the presence of 39.7 mg/L [Formula: see text] indicated that 71.2% [Formula: see text] was removed by converting to N2 (46.3%) and assimilating as biomass (42.5%). Substances such as [Formula: see text], [Formula: see text] and N2O were detected at very low concentrations. Ammonium mono-oxygenase, hydroxylamine oxidase, nitrite reductase and nitrate reductase activity were measured. The ammonium removal pathway of the strain was speculated to be [Formula: see text].

Kinetic characteristics and N2O production of a heterotrophic nitrifying bacterium Pseudomonas putida YH capable of tolerating adverse environmental conditions

“…As the initial concentration increased to 100 and 200 mg L −1 , the NH 4 + –N removal efficiencies reached 99.30% and 99.83%, respectively, and an obvious improvement of cellular growth also was observed because OD 600 peaked at 1.36 and 1.85, respectively. Under high initial NH 4 + –N concentration (500 mg L −1 ), the removal efficiency of NH 4 + –N was 66.95%, which was much greater than Pseudomonas fluorescens wsw‐1001, and the maximum OD 600 further increased to 1.92. Furthermore, the increase of R m demonstrated that strain YH was capable of tolerating wide ranges of NH 4 + –N concentrations with efficient nitrogen removal, whereas a longer adaption period was necessary for treating ammonium‐rich wastewater.…”

Heterotrophic Nitrification-Aerobic Denitrification Performance of Strain Y-12 under Low Temperature and High Concentration of Inorganic Nitrogen Conditions

“…Paracoccus versutus LYM [5] could not utilize NO 2 − -N as a sole nitrogen source unless NH 4 + -N existed.…”

“…4 [14], Acinetobacter calcoaceticus HNR [12], Paracoccus versutus LYM [5], and Alcaligenes faecalis NR [15]. Alcaligenes faecalis No.…”

“…Moreover, excessive nitrogen in water bodies may do harm to both aquatic life and human beings. For instance, NH 4 + -N damages the liver and kidney of fishes, NO 3 − -N destroys the immune system of fishes, and NO 2 − -N can cause severe health problems such as methemoglobinemia in infants and even cancer in humans [3][4][5]. Therefore, finding a method to remove the undesirable nitrogen is essential and urgent.…”

Simultaneous bisphenol F degradation, heterotrophic nitrification and aerobic denitrification by a bacterial consortium

“…To date, many HN‐AD bacteria have been isolated and exhibit good performance for nitrogen removal under various conditions, even including low temperature or high salt condition . These HN‐AD bacteria generally remove nitrogen by using readily biodegradable carbon sources such as glucose, sodium acetate, sodium succinate, sodium citrate and corn flour . In fact, refractory aromatic hydrocarbon pollutants and nitrate/ammonia are commonly found in wastewaters .…”