Ammonium triggered the response mechanism of lysine crotonylome in tea plants

Sun, Jianhao; Qiu, Chen; Qian, Wenjun; Sun, Litao; Li, Yusheng; Ding, Zhaotang

doi:10.1186/s12864-019-5716-z

Cited by 46 publications

(43 citation statements)

References 42 publications

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“…Comparing these conserved motifs identi ed in chrysanthemums with other plants, a large number of conserved motifs shared with chrysanthemum were found ( Figure 6, Table S9) [22][23][24]26]. It is worth noting that chrysanthemum and four other studied plants contain the same conserved KcrD, KcrE, and Ekcr.…”

Section: Motif Analysis Of Lysine Crotonylation Peptidesmentioning

confidence: 99%

Proteome-wide and lysine crotonylation profiling reveals the importance of crotonylation in chrysanthemum (Dendranthema grandiforum) under low-temperature

Lin

Bai

et al. 2020

Preprint

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Background: Low-temperature severely affects the growth and development of chrysanthemum which is one kind of ornamental plant well-known and widely used in the world. Lysine crotonylation is a recently identified post-translational modification (PTM) with multiple cellular functions. However, lysine crotonylation under low-temperature stress has not been studied. Results: Proteome-wide and lysine crotonylation of chrysanthemum at low temperature was analyzed using TMT labeling, sensitive immuno-precipitation, and high-resolution LC-MS/MS. The results showed that 2017 crotonylation sites were identified in 1199 proteins. Treatment at 4 °C for 24 h and −4 °C for 4 h resulted in 393 upregulated proteins and 500 downregulated proteins (1.2-fold threshold and P < 0.05). Analysis of biological information showed that lysine crotonylation was involved in photosynthesis, ribosomes, and antioxidant systems. The crotonylated proteins and motifs in chrysanthemum were compared with other plants to obtain orthologous proteins and conserved motifs. To further understand how lysine crotonylation at K136 affected APX (ascorbate eroxidase), we performed a site-directed mutation at K136 in APX. Site-directed crotonylation showed that lysine decrotonylation at K136 reduced APX activity, and lysine complete crotonylation at K136 increased APX activity. Conclusion: In summary, our study comparatively analyzed proteome-wide and crotonylation in chrysanthemum under low-temperature stress and provided insights into the mechanisms of crotonylation in positively regulated APX activity to reduce the oxidative damage caused by low-temperature stress. These data provided an important basis for studying crotonylation to regulate antioxidant enzyme activity in response to low-temperature stress and a new research ideas for chilling-tolerance and freezing-tolerance chrysanthemum molecular breeding.

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Section: Motif Analysis Of Lysine Crotonylation Peptidesmentioning

confidence: 99%

Proteome-wide and lysine crotonylation profiling reveals the importance of crotonylation in chrysanthemum (Dendranthema grandiforum) under low-temperature

Lin

Bai

et al. 2020

Preprint

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“…Therefore, we used tandem mass tag (TMT) and liquid chromatography-mass spectrometry (LC-MS) to study the global profile of Protein extraction and trypsin digestion. The protein extraction and digestion of the samples were performed according to previous methods 17,18 . In brief, four volumes of lysis buffer were added to the samples, followed by sonication and centrifugation.…”

mentioning

confidence: 99%

Drought stress triggers proteomic changes involving lignin, flavonoids and fatty acids in tea plants

Wang

Xie

et al. 2020

Sci Rep

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Drought stress triggers a series of physiological and biochemical changes in tea plants. It is well known that flavonoids, lignin and long-chain fatty acids play important roles in drought resistance. However, changes in proteins related to these three metabolic pathways in tea plants under drought stress have not been reported. We analysed the proteomic profiles of tea plants by tandem mass tag and liquid chromatography-tandem mass spectrometry. A total of 4789 proteins were identified, of which 11 and 100 showed up- and downregulation, respectively. The proteins related to the biosynthesis of lignin, flavonoids and long-chain fatty acids, including phenylalanine ammonia lyase, cinnamoyl-CoA reductase, peroxidase, chalcone synthase, flavanone 3-hydroxylase, flavonol synthase, acetyl-CoA carboxylase 1,3-ketoacyl-CoA synthase 6 and 3-ketoacyl-CoA reductase 1, were downregulated. However, the contents of soluble proteins, malondialdehyde, total phenols, lignin and flavonoids in the tea plants increased. These results showed that tea plants might improve drought resistance by inhibiting the accumulation of synthases related to lignin, flavonoids and long-chain fatty acids. The proteomic spectrum of tea plants provides a scientific basis for studying the pathways related to lignin, flavonoid and long-chain fatty acid metabolism in response to drought stress.

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“…Microscopy and ELISAs were used to estimate the integrity and purity of the isolated chloroplast. ELISAs is a powerful method for measuring the enzymatic activity and is often applied in biochemical assays not only for animal cells but also for plants and algae [42,43]. ELISAs are rapid, sensitive, speci c, and precise [44], therefore, we used it to quantify enzymatic activity.…”

Section: Discussionmentioning

confidence: 99%

Isolation of chloroplasts from marine microalgae Isochrysis galbana Parke suitable for organelle lipid composition analysis

Ye¹,

Li²,

Xu³

et al. 2020

Preprint

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Background: Marine microalgae, Isochrysis galbana Parke, is an important diet microalgal species with high nutritional value. Different from other unicellular microalgae, its cell contains two chloroplasts which are the major sites for lipid synthesis. Results: Here, we optimized a chloroplast isolation approach suitable for I. galbana Parke, and evaluated the purity and integrity of the isolated chloroplasts by microscopic observations and biochemical assays. The chloroplast lipids were sequenced by ultrahigh-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). Glycolipids were the main chloroplast lipids, and DGDG, MGDG, and SQDG were the most abundant glyceroglycolipids. DGMG and SQMG were not observed among the chloroplast lipids. In addition, DG was the most abundant neutral lipid. A part of fatty acyl R1/R2 with MGMGs, DGDGs, MGDGs, SQDGs, PEs, and PCs were not found in chloroplasts. The fatty acid proportion of chloroplast lipids were increased, decreased, or remained unchanged compared with the whole-cell. Conclusions: This newly developed isolation approach was a simple and reliable method to isolate chloroplasts with high integrity and purity. Collectively, our findings show that such an isolation approach may be used in studies on many different aspects of chloroplast biology, and offers a useful reference for isolation of chloroplast from other marine microalgae.

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Ammonium triggered the response mechanism of lysine crotonylome in tea plants

Cited by 46 publications

References 42 publications

Proteome-wide and lysine crotonylation profiling reveals the importance of crotonylation in chrysanthemum (Dendranthema grandiforum) under low-temperature

Proteome-wide and lysine crotonylation profiling reveals the importance of crotonylation in chrysanthemum (Dendranthema grandiforum) under low-temperature

Drought stress triggers proteomic changes involving lignin, flavonoids and fatty acids in tea plants

Isolation of chloroplasts from marine microalgae Isochrysis galbana Parke suitable for organelle lipid composition analysis

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