Amniotic Membrane as a Carrier for Cultivated Human Corneal Endothelial Cell Transplantation

Ishino, Y.; Sano, Yoichiro; Nakamura, Takahiro; Connon, Che J.; Rigby, Helen; Fullwood, Nigel J.; Kinoshita, Shigeru

doi:10.1167/iovs.03-0016

Cited by 296 publications

(237 citation statements)

References 19 publications

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“…TE-HCE transplants should provide an effective treatment for PCEP. This finding is in accordance with previous reports (Ishino et al, 2004;Fan et al, 2009b;2010b). The eye pressures of TE-HCE, PKP, and control eyes fluctuated from time to time.…”

Section: Discussionsupporting

confidence: 94%

“…TE-HCE transplants were capable of recovering the corneal transparency of PCEP rabbits, maintaining the transparency of corneas for longer than previously reported (Aboalchamat et al, 1999;Ishino et al, 2004;Fu et al, 2006;Koizumi et al, 2008;Fan et al, 2009b;2010b;Proulx et al, 2009). The corneas of TE-HCE eyes became thinner and thinner with the time, while the corneas of PCEP and PKP eyes remained almost unchanged in thickness.…”

Section: Discussionmentioning

confidence: 61%

“…Diverse types of cells and various scaffold carriers have been combined previously and a therapeutic efficiency of about one week of maintenance of corneal transparency has been attained in rabbit (Aboalchamat et al, 1999;Ishino et al, 2004;Koizumi et al, 2008;Fan et al, 2009b;Proulx et al, 2009). Unfortunately, all the previous trials fell well short of a clinical therapy for PCEP; the immortalized seed cells were either potentially tumorigenic or limited in number after primary culture or 4-5 rounds of subculture (Fan et al, 2007;2009a).…”

Section: Introductionmentioning

confidence: 99%

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Therapeutic efficiency of tissue-engineered human corneal endothelium transplants on rabbit primary corneal endotheliopathy

Fan

Zhao

et al. 2011

J. Zhejiang Univ. Sci. B

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Abstract:To evaluate the therapeutic efficiency of tissue-engineered human corneal endothelia (TE-HCEs) on rabbit primary corneal endotheliopathy (PCEP), TE-HCEs reconstructed with monoclonal human corneal endothelial cells (mcHCECs) and modified denuded amniotic membranes (mdAMs) were transplanted into PCEP models of New Zealand white rabbits using penetrating keratoplasty. The TE-HCEs were examined using diverse techniques including slit-lamp biomicroscopy observation and pachymeter and tonometer measurements in vivo, and fluorescent microscopy, alizarin red staining, paraffin sectioning, scanning and transmission electron microscopy observations in vitro. The corneas of transplanted eyes maintained transparency for as long as 200 d without obvious edema or immune rejection. The corneal thickness of transplanted eyes decreased gradually after transplanting, reaching almost the thickness of normal eyes after 156 d, while the TE-HCE non-transplanted eyes were turbid and showed obvious corneal edema. The polygonal corneal endothelial cells in the transplanted area originated from the TE-HCE transplant. An intact monolayer corneal endothelium had been reconstructed with the morphology, cell density and structure similar to those of normal rabbit corneal endothelium. In conclusion, the transplanted TE-HCE can reconstruct the integrality of corneal endothelium and restore corneal transparency and thickness in PCEP rabbits. The TE-HCE functions normally as an endothelial barrier and pump and promises to be an equivalent of HCE for clinical therapy of human PCEP.

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Section: Discussionsupporting

confidence: 94%

Section: Discussionmentioning

confidence: 61%

Section: Introductionmentioning

confidence: 99%

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Therapeutic efficiency of tissue-engineered human corneal endothelium transplants on rabbit primary corneal endotheliopathy

Fan

Zhao

et al. 2011

J. Zhejiang Univ. Sci. B

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“…It has also been demonstrated that denuded AM can be utilized as a feeder layer for some stem cells and may be used for neuronal differentiation (Miyamoto et al, 2004;Ueno et al, 2006;Meng et al, 2007). Denuded AM has been investigated as a carrier of chondrocytes, and it has been suggested that the AM can serve as a carrier matrix for cartilage regeneration (Jin et al, 2007). When epithelial and mesenchymal cells are seeded on a cellular scaffold created from the AM, the cells were highly interconnected and capable of penetrating the porous structure of the amnion scaffold.…”

Section: The Amniotic Membrane As a Scaffold For Tementioning

confidence: 99%

Properties of the amniotic membrane for potential use in tissue engineering

Niknejad

Peirovi²,

Jorjani³

et al. 2008

eCM

685

757

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An important component of tissue engineering (TE) is the supporting matrix upon which cells and tissues grow, also known as the scaffold. Scaffolds must easily integrate with host tissue and provide an excellent environment for cell growth and differentiation. Most scaffold materials are naturally derived from mammalian tissues. The amniotic membrane (AM) is considered an important potential source for scaffolding material. The AM represents the innermost layer of the placenta and is composed of a single epithelial layer, a thick basement membrane and an avascular stroma. The special structure and biological viability of the AM allows it to be an ideal candidate for creating scaffolds used in TE. Epithelial cells derived from the AM have the advantages of stem cells, yet are a more suitable source of cells for TE than stem cells. The extracellular matrix components of the basement membrane of the AM create an almost native scaffold for cell seeding in TE. In addition, the AM has other biological properties important for TE, including anti-inflammatory, anti-microbial, anti-fibrosis, anti-scarring, as well as reasonable mechanical property and low immunogenicity. In this review, the various properties of the AM are discussed in light of their potential use for TE.

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“…Materials like collagen, amniotic membrane, and biodegradable polymers have successfully been used in culture media and animal models. [80][81][82] Porcine corneal matrix and Descemet's membrane have also been used as scaffolds for transplanted cells allowing good transparency in cat and rat corneas, and maintaining cell properties after the transplant. 60,83 Human decellularized stromas have been used to culture HCECs, allowing them to retain the expression of Na þ /K þ -ATPase and ZO-1 markers for greater than 14 days.…”

Section: Cell Therapymentioning

confidence: 99%

Corneal endothelium: developmental strategies for regeneration

Zavala

Jaime

Barrientos

et al. 2013

Eye

113

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The main treatment available for restoration of the corneal endothelium is keratoplasty. This procedure is faced with several difficulties, including the shortage of donor tissue, postsurgical complications associated with the use of drugs to prevent immune rejection, and a significant increase in the occurrence of glaucoma. Recently, surgical procedures such as Descemet's stripping endothelial keratoplasty have focused on the transplant of corneal endothelium, yielding better visual results but still facing the need for donor tissue. The emergent strategies in the field of cell biology and tissue cultivation of corneal endothelial cells aim at the production of transplantable endothelial cell sheets. Cell therapy focuses on the culture of corneal endothelial cells retrieved from the donor, in the donor's cornea, followed by transplantation into the recipient. Recently, research has focused on overcoming the challenge of harvesting human corneal endothelial cells and the generation of new biomembranes to be used as cell scaffolds in surgical procedures. The use of corneal endothelial precursors from the peripheral cornea has also demonstrated to be effective and represents a valuable tool for reducing the risk of rejection in allogeneic transplants. Several animal model reports also support the use of adult stem cells as therapy for corneal diseases. Current results represent important progresses in the development of new strategies based on alternative sources of tissue for the treatment of corneal endotheliopathies. Different databases were used to search literature: PubMed, Google Books, MD Consult, Google Scholar, Gene Cards, and NCBI Books. The main search terms used were: 'cornea AND embryology AND transcription factors', 'human endothelial keratoplasty AND risk factors', '(cornea OR corneal) AND (endothelium OR endothelial) AND cell culture', 'mesenchymal stem cells AND cell therapy', 'mesenchymal stem cells AND cornea', and 'stem cells AND (cornea OR corneal) AND (endothelial OR endothelium)'.

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Amniotic Membrane as a Carrier for Cultivated Human Corneal Endothelial Cell Transplantation

Abstract: The cell density and morphology of cHCECs on AM were similar to those of normal corneas, and cHCECs on AM were functional in vivo. These results indicate that AM maintains HCEC morphology and function and could serve as a carrier for cHCEC transplantation.

Cited by 296 publications

References 19 publications

Therapeutic efficiency of tissue-engineered human corneal endothelium transplants on rabbit primary corneal endotheliopathy

Therapeutic efficiency of tissue-engineered human corneal endothelium transplants on rabbit primary corneal endotheliopathy

Properties of the amniotic membrane for potential use in tissue engineering

Corneal endothelium: developmental strategies for regeneration

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