Amniotic membrane: New concepts for an old dressing

İlhan, Abdullah; Yolcu, Ümit; Gündoğan, Fatih Ç.

doi:10.1111/wrr.12256

Cited by 3 publications

(2 citation statements)

References 6 publications

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“…Furthermore, the amniotic membrane plays a coordinated role with MSC to promote burn wound healing. The properties of the human amniotic membrane in promoting wound healing, skin tissue reconstruction, and regulating inflammation and microbial growth have been widely recognized by hundreds of clinical trials or pre-clinical papers, including the domains of burns and chronic ulcer treatment, peritoneal reconstruction, tendon repair, microvascular reconstruction, and corneal repair [ [31] , [32] , [33] , [34] , [35] , [36] , [37] , [38] , [39] , [40] ]. In our previous studies, we made great progress in upgrading the preparation methods for human amniotic membranes and found that decellularized amniotic membranes could be used to construct natural microcarriers for stem cells.…”

Section: Introductionmentioning

confidence: 99%

Acceleration of burn wound healing by micronized amniotic membrane seeded with umbilical cord-derived mesenchymal stem cells

Zhou,

Xun,

et al. 2023

Materials Today Bio

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Section: Introductionmentioning

confidence: 99%

Acceleration of burn wound healing by micronized amniotic membrane seeded with umbilical cord-derived mesenchymal stem cells

Zhou,

Xun,

et al. 2023

Materials Today Bio

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“…The human placenta, especially the amniotic membrane, has gained interest due to its favorable characteristics. Placenta tissue is non-immunogenic [ 42 , 43 ] and amnion has already been used as wound dressings in different clinical applications [ [43] , [44] , [45] ]. HuBiogel™, a commercially available ECM hydrogel derived from human amnion, was already used for vascular graft coating and showed satisfying endothelialization in-vitro [ 46 ].…”

Section: Introductionmentioning

confidence: 99%

Chorion-derived extracellular matrix hydrogel and fibronectin surface coatings show similar beneficial effects on endothelialization of expanded polytetrafluorethylene vascular grafts

Rohringer

Schneider

Eder

et al. 2022

Materials Today Bio

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FGF-2-Induced Human Amniotic Mesenchymal Stem Cells Seeded on a Human Acellular Amniotic Membrane Scaffold Accelerated Tendon-to-Bone Healing in a Rabbit Extra-Articular Model

Zhang

Liu

et al. 2020

Stem Cells International

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Background. FGF-2 (basic fibroblast growth factor) has a positive effect on the proliferation and differentiation of many kinds of MSCs. Therefore, it represents an ideal molecule to facilitate tendon-to-bone healing. Nonetheless, no studies have investigated the application of FGF-2-induced human amniotic mesenchymal stem cells (hAMSCs) to accelerate tendon-to-bone healing in vivo. Objective. The purpose of this study was to explore the effect of FGF-2 on chondrogenic differentiation of hAMSCs in vitro and the effect of FGF-2-induced hAMSCs combined with a human acellular amniotic membrane (HAAM) scaffold on tendon-to-bone healing in vivo. Methods. In vitro, hAMSCs were transfected with a lentivirus carrying the FGF-2 gene, and the potential for chondrogenic differentiation of hAMSCs induced by the FGF-2 gene was assessed using immunofluorescence and toluidine blue (TB) staining. HAAM scaffold was prepared, and hematoxylin and eosin (HE) staining and scanning electron microscopy (SEM) were used to observe the microstructure of the HAAM scaffold. hAMSCs transfected with and without FGF-2 were seeded on the HAAM scaffold at a density of 3 × 10 5 cells/well. Immunofluorescence staining of vimentin and phalloidin staining were used to confirm cell adherence and growth on the HAAM scaffold. In vivo, the rabbit extra-articular tendon-to-bone healing model was created using the right hind limb of 40 New Zealand White rabbits. Grafts mimicking tendon-to-bone interface (TBI) injury were created and subjected to treatment with the HAAM scaffold loaded with FGF-2induced hAMSCs, HAAM scaffold loaded with hAMSCs only, HAAM scaffold, and no special treatment. Macroscopic observation, imageological analysis, histological assessment, and biomechanical analysis were conducted to evaluate tendon-tobone healing after 3 months. Results. In vitro, cartilage-specific marker staining was positive for the FGF-2 overexpression group. The HAAM scaffold displayed a netted structure and mass extracellular matrix structure. hAMSCs or hAMSCs transfected with FGF-2 survived on the HAAM scaffold and grew well. In vivo, the group treated with HAAM scaffold loaded with FGF-2-induced hAMSCs had the narrowest bone tunnel after three months as compared with other groups. In addition, macroscopic and histological scores were higher for this group than for the other groups, along with the best mechanical strength. Conclusion. hAMSCs transfected with FGF-2 combined with the HAAM scaffold could accelerate tendon-to-bone healing in a rabbit extraarticular model.

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Amniotic membrane: New concepts for an old dressing

Cited by 3 publications

References 6 publications

Acceleration of burn wound healing by micronized amniotic membrane seeded with umbilical cord-derived mesenchymal stem cells

Acceleration of burn wound healing by micronized amniotic membrane seeded with umbilical cord-derived mesenchymal stem cells

Chorion-derived extracellular matrix hydrogel and fibronectin surface coatings show similar beneficial effects on endothelialization of expanded polytetrafluorethylene vascular grafts

FGF-2-Induced Human Amniotic Mesenchymal Stem Cells Seeded on a Human Acellular Amniotic Membrane Scaffold Accelerated Tendon-to-Bone Healing in a Rabbit Extra-Articular Model

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