Amoxycillin as an alternative to dihydrostreptomycin sulphate for treating cattle infected with Leptospira borgpetersenii serovar hardjo

Inactivation of leptospires in pools of semen from three Holstein Friesian bulls, collected in an artificial vagina, was investigated. Spermatic concentration was adjusted in egg yolk citrate extender, submitted to the following treatments: A (control; without antibiotics); B (penicillin, 1,000 UI/mL -streptomycin, 1,000 µg/mL); C (amoxicillin, 1,000 µg/mL); D (ceptiofur sodium, 1,000 µg/mL); E (amoxicillin 1,000 µg/mL -ceptiofur sodium 1,000 µg/mL). Leptospires (2.0 x 10 6 leptospires/mL) were added into the diluted semen. Recovery of leptospires was obtained in modified EMJH semi-solid medium with and without antibiotics. The antibiotics in the concentrations used did not affect means of percentage of progressive motility and individual progressive motility of spermatozoids. Penicillin-streptomycin presented the best results in leptospire inactivation (97.1%). Amoxicillin, ceptiofur sodium and their combination at the concentrations studied presented poor results: 59.29%; 32.5% and 60.36% of inactivation, being less effective in leptospire inactivation than penicillinstreptomycin.

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“…Smith (38) has described a strong effect of amoxicillin on the "in vivo" treatment of kidney carriers, in cattle infected by the serovar hardjo.…”

Section: (1)mentioning

confidence: 99%

Comparison of four antibiotics for inactivating leptospires in bull semen diluted in egg yolk extender and experimentally inoculated with Leptospira santarosai serovar guaricura

Miraglia

Morais

Cortéz

et al. 2003

Braz. J. Microbiol.

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“…Leptospira-infected humans are often treated with ␤-lactam antibiotics. It has recently been suggested that leptospirosis in livestock can be treated with ␤-lactam antibiotics (19). In vitro, pathogenic leptospires are very sensitive to ␤-lactam antibiotics (16).…”

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confidence: 99%

Penicillin-Binding Proteins in Leptospira interrogans

Brenot

Trott

Girons

et al. 2001

Antimicrob Agents Chemother

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The Leptospira interrogans ponA and pbpB genes were isolated and characterized. ponA and pbpB encode the penicillin-binding proteins (PBPs) 1 and 3, respectively. There is little sequence variation between the PBP genes from two L. interrogans strains (serovar icterohaemorrhagiae strain Verdun and serovar pomona strain RZ11). The deduced L. interrogans PBP 1 and PBP 3 protein sequences from the two strains shared over 50% similarity to homologous proteins from Escherichia coli. It was demonstrated for strain Verdun that ponA and pbpB are transcribed individually from their own promoter. The ponA and pbpB genes from both strains are separated by 8 to 10 kb and oriented such that their transcription is convergent. The L. interrogans PBP 1 and PBP 3 proteins were synthesized in E. coli and were modified with ampicillin using a digoxigenin-ampicillin conjugate. These data show that both genes encode functional PBPs.

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“…As Mycotil, it is used to treat bovine respiratory disease associated with pasteurellae [16,23]. Tilmicosin (10 mg/kg, once) and amoxicillin (15 mg/ml, once or twice) were effective in cattle for resolving experimental leptospirosis caused by L. borgpetersenii serovar Hardjo [3,32]. Enrofloxacin was used concurrently with ampicillin in canine leptospirosis and 83% of infected dogs survived [1].…”

Section: Discussionmentioning

confidence: 99%

In vitrosusceptibilities ofLeptospiraspp. andBorrelia burgdorferiisolates to amoxicillin, tilmicosin, and enrofloxacin

et al. 2006

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Antimicrobial susceptibility testing was conducted with 6 different spirochetal strains (4 strains of Leptospira spp. and 2 strains of Borrelia burgdorferi) against 3 antimicrobial agents, commonly used in equine and bovine practice. The ranges of MIC and MBC of amoxicillin against Leptospira spp. were 0.05-6.25 µg/ml and 6.25-25.0 µg/ml, respectively. And the ranges of minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) of amoxicillin against B. burgdorferi were 0.05-0.39 µg/ml and 0.20-0.78 µg/ml, respectively. The ranges of MIC and MBC of enrofloxacin against Leptospira spp. were 0.05-0.39 µg/ml and 0.05-0.39 µg/ml, respectively. Two strains of B. burgdorferi were resistant to enrofloxacin at the highest concentration tested for MBC (≥100 µg/ml). Therefore, the potential role of tilmicosin in the treatment of leptospirosis and borreliosis should be further evaluated in animal models to understand whether the in vivo studies will confirm in vitro results. All spirochetal isolates were inhibited (MIC) and were killed (MBC) by tilmicosin at concentrations below the limit of testing (≤0.01 µg/ml).

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Amoxycillin as an alternative to dihydrostreptomycin sulphate for treating cattle infected with Leptospira borgpetersenii serovar hardjo

Abstract: Amoxycillin may be an acceptable alternative to dihydrostreptomycin sulphate for the treatment of cattle infected with L hardjo.

Cited by 22 publications

References 8 publications

Comparison of four antibiotics for inactivating leptospires in bull semen diluted in egg yolk extender and experimentally inoculated with Leptospira santarosai serovar guaricura

Comparison of four antibiotics for inactivating leptospires in bull semen diluted in egg yolk extender and experimentally inoculated with Leptospira santarosai serovar guaricura

Penicillin-Binding Proteins in Leptospira interrogans

In vitrosusceptibilities ofLeptospiraspp. andBorrelia burgdorferiisolates to amoxicillin, tilmicosin, and enrofloxacin

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