&amp;lt;i&amp;gt;In vitro&amp;lt;/i&amp;gt; analysis of T cell responses induced by breast tumor cell lysate pulsed with autologous dendritic cells

Delirezh, Nowruz; Moazzeni, Seyed Mohammad; Shokri, Fazel; Shokrgozar, Mohammad Ali; Atri, Morteza Morteza; Karbassian, Hamid

doi:10.4236/abb.2012.32019

Cited by 3 publications

(2 citation statements)

References 38 publications

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“…In this study, DCs were successfully generated from cryo CB in culture medium containing GM-CFS, IL-4, and TNF-α, which consistently showed a DC marker expression profile, such as CD80high, CD86high, CD40high, HLA-DRhigh, CD123low CD56low, and CD14low. Cryo CBMDCs expressed a typical DC morphology with thin and long dendrites, which have been described in previous reports [21,22,[26][27][28][29][30]. Thus, our results indicate that cryo CB can be a potential source for DC generation, which then may be developed into immunotherapies.…”

Section: Discussionsupporting

confidence: 85%

Induction of Antitumor Immunity by Exosomes Isolated from Cryopreserved Cord Blood Monocyte-Derived Dendritic Cells

Than

Hoang

et al. 2020

IJMS

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(1) Background: Dendritic cell (DC) vaccination has shown outstanding achievements in cancer treatment, although it still has some adverse side effects. Vaccination with DC-derived exosomes has been thought to overcome the side effects of the parental DCs. (2) Method: We performed the experiments to check the ability of cryopreserved umbilical cord blood mononuclear cell-derived DCs (cryo CBMDCs) and their exosomes to prime allogeneic T cell proliferation and allogeneic peripheral blood mononuclear cell (alloPBMCs) cytotoxicity against A549 lung cancer cells. (3) Results: We found that both lung tumor cell lysate-pulsed DCs and their exosomes could induce allogeneic T cell proliferation. Moreover, alloPBMCs primed with tumor cell lysate-pulsed DCs and their exosomes have a greater cytotoxic activity against A549 cells compared to unprimed cells and cells primed with unpulsed DCs and their exosomes. (4) Conclusion: Tumor cell lysate-pulsed DCs and their exosomes should be considered to develop into a novel immunotherapeutic strategy—e.g., vaccines—for patients with lung cancer. Our results also suggested that cryo umbilical cord blood mononuclear cells source, which is a readily and available source, is effective for generation of allogeneic DCs and their exosomes will be material for vaccinating against cancer.

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Section: Discussionsupporting

confidence: 85%

Induction of Antitumor Immunity by Exosomes Isolated from Cryopreserved Cord Blood Monocyte-Derived Dendritic Cells

Than

Hoang

et al. 2020

IJMS

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“…The heat shock soluble proteins obtained by the freeze-thaw method increased antigen presentation and expression of helper stimulating molecules in DCs [50]. As an example of this method, the in vitro and in vivo immunotherapeutic efficacy of a vaccine formulation prepared by loading freeze-thawed antigens of BC cells onto DCs was evaluated by Delirezh et al [51]. The study concluded that both CD4 + and CD8 + T lymphocyte-mediated immunity was acquired in vitro .…”

Section: General Approaches On Vaccine Development Against Breast Cancermentioning

confidence: 99%

Current Approaches in Development of Immunotherapeutic Vaccines for Breast Cancer

et al. 2018

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Cancer is the leading cause of death worldwide. In developed as well as developing countries, breast cancer is the most common cancer found among women. Currently, treatment of breast cancer consists mainly of surgery, chemotherapy, hormone therapy, and radiotherapy. In recent years, because of increased understanding of the therapeutic potential of immunotherapy in cancer prevention, cancer vaccines have gained importance. Here, we review various immunotherapeutic breast cancer vaccines including peptide-based vaccines, whole tumor cell vaccines, gene-based vaccines, and dendritic cell vaccines. We also discuss novel nanotechnology-based approaches to improving breast cancer vaccine efficiency.

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Mannosylated polylactic-co-glycolic acid (MN-PLGA) nanoparticles induce potent anti-tumor immunity in murine model of breast cancer

Sheikhzadeh

Delirezh

Hobbenaghi

2021

Biomedicine & Pharmacotherapy

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<i>In vitro</i> analysis of T cell responses induced by breast tumor cell lysate pulsed with autologous dendritic cells

Abstract: ABSTRACT

Cited by 3 publications

References 38 publications

Induction of Antitumor Immunity by Exosomes Isolated from Cryopreserved Cord Blood Monocyte-Derived Dendritic Cells

Induction of Antitumor Immunity by Exosomes Isolated from Cryopreserved Cord Blood Monocyte-Derived Dendritic Cells

Current Approaches in Development of Immunotherapeutic Vaccines for Breast Cancer

Mannosylated polylactic-co-glycolic acid (MN-PLGA) nanoparticles induce potent anti-tumor immunity in murine model of breast cancer

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