Ampelographic and molecular characterisation of grapevine varieties in the gene bank of the experimental vineyard ‘Radmilovac’ – Serbia

Abstract

Characterisations of thirty grapevine varieties (Vitis vinifera L.) from the experimental vineyard ‘Radmilovac’ were conducted using a large number of OIV descriptors and eight highly polymorphic microsatellite loci. The ampelographic description contained 45 features. Molecular characterisation of selected microsatellite loci was performed using capillary electrophoresis fragment analysis. Dendrograms based on ampelographic and genetic data resulted in three groups of varieties. Qualitative ampelographic characteristics tended to manifest significant differences. The most common deviation among varieties within the group was in the characteristic OIV 051 (colouration of the upper side of a young leaf). Genetic characterisation of SSR markers through analyses of a large number of varieties contributes to better organisation of grapevine collections and simpler identification of varieties, as well as data exchange. When identifying the varieties, the results of the DNA analysis should be combined with the ampelographic descriptors, in order to select grapevine varieties with desirable viticultural and oenological traits. Integration of the obtained genetic data with the ampelographic data is of utmost importance for accurate identification of the varieties and offers a significant means for the preservation and use of the varieties.

Screening of Galician grapevine varieties by SNPs, phenotypic traits, and phytopathology

“…High diversity was found at a morphological level, as only 6 out of 74 ampelographic and ampelometric parameters studied were homogeneous for all varieties. This high morphological diversity has also been reported in other collections (Lamine et al, 2014;Zinelabidine et al, 2014;Khalil et al, 2017;Abiri et al, 2020;Milisǐćet al, 2021). The variation explained by the first three axes of the PCA using ampelographic, ampelometric, and leaf disc test data was low and similar to that obtained by Merkouropoulos et al (2015) in an ampelographic study in a Greek grapevine collection.…”

The genetic characterization of grapevines prospected in old Serbian vineyards reveals multiple relationships between traditional varieties of the Balkans

“…The term “bojadiser” means teinturier in Serbian, and “Župski Teinturier” is used as a synonym of ‘Župski Bojadiser’. Thus, ‘Tinturier Youpski’ is very likely a synonym of ‘Župski Bojadiser’, a teinturier variety bred in 1979 at the University of Belgrade ( Milišić et al., 2021 ). However, the sample “ Unknown SRB #03 ” does not correspond to a specimen of ‘Župski Bojadiser’, since it does not have red-fleshed berries.…”

“…After this crisis, devastated vineyards were replanted with grafted vines ( Garić Petrović, 2020 ), commonly replacing local varieties by foreign varieties to accommodate to international market trends. Today, the Serbian wine industry still relies on the cultivation of a reduced number of international varieties, like ‘Cabernet Sauvignon’, ‘Merlot’, ‘Chardonnay’, and ‘Sauvignon Blanc’, and autochthonous varieties like ‘Prokupac’ represent a minor fraction of the Serbian vineyard ( Milišić et al., 2021 ). Nevertheless, there is a raising interest in the identification and promotion of the cultivation of indigenous and regional varieties like ‘Smederevka’ (syn.…”

HRM Tekniği ile Asma Çeşitlerinin Belirlenmesi ve Farklı Kullanım Alanları

“…İlk kez 1661 yılında Sachs tarafından kullanılan "Ampelografi", üzüm bitkisinin tanımlanması ve sınıflandırılmasıyla ilgilenen bir bilim dalı olarak ortaya çıkmıştır [18]. Daha sonra Türkiye'de ve dünyada yaygın olarak bağcılık yapılan diğer ülkelerde de üzüm çeşitlerinin tanımlanması ve morfolojik, pomolojik özelliklerinin belirlenmesine yönelik birçok çalışma yürütülmüştür [19,20,21,22,23,24] [36,37,38,39,40]. Geçtiğimiz 25 yılda Türkiye'de florasında yer alan ve kültürü yapılan asma tür ve çeşitlerinin moleküler markörlerle tanımlanmasına yönelik çalışmalara hız verilmiş ve RFLP, AFLP, RAPD, SSR ve ISSR yöntemleri kullanılan araştırmalar farklı araştırmacılar tarafından yürütülmüştür [41,42,43,44,45,46,47].…”