Amperometric bioelectrode for specific human immunoglobulin G determination: Optimization of the method to diagnose American trypanosomiasis

A mediatorless and label-free amperometric immunosensor for detection of h-IgG

“…The experimental results testify that the label-free and mediatorless immunosensor shows specific current response toward h-IgG in the range 0.82-90 ng mL −1 with a detection limit of 0.25 ng mL −1 by differential pulse voltammograms (DPV). The modified electrode exhibits high sensitivity, low detection limit and inherent simplicity than other reported papers [27][28]. This is attributed to the properties of nano-Au.…”

The role of nanogold in human tropical diseases: research, detection and therapy

“…In a very similar approach, Ribone and co-workers [28] prepared bioelectrodes to detect IgG antibodies occurring in sera of patients suffering from American trypanosomiasis. The main difference from the previous description [27], is the use of two different thiols, 3-mercapto-1-propionic acid (MPA), 3-mercapto-1-propanesulfonic acid (MPSA), which provide sulfonate or carboxylic residues, for the electrostatic binding of the antigen.…”

“…This device was planned for the canine visceral form; however this assembling seems to be promising also for human leishmaniasis. It is important to distinguish from the similar examples given before [27,28], as no immunotechnique is applied to sensor construction in this case. The amine groups of cysteamine provided reaction sites for covalently bind to the glutaraldehyde.…”

A Novel Fluorescence Immunoassay Based on Two-time Amplified Fluorescence Signal by Hemin and Magnetic Nanoparticles for the Detection of Antigen

“…The detection limit (3r) calculated from the standard deviation of the blank (n ¼ 3) was 9.8 ng mL À1 , which is comparable to or better than other immunoassay formats (Li et al 2007;Ribone et al 2006;Zhao, Huang, and Li 2006).…”