Amperometric carbohydrate antigen 19-9 immunosensor based on three dimensional ordered macroporous magnetic Au film coupling direct electrochemistry of horseradish peroxidase

Zhang, Qi; Chen, Xiaojun; Tang, Yin; Ge, Lingna; Guo, Buhua; Yang, Cheng

doi:10.1016/j.aca.2014.01.033

Cited by 34 publications

(17 citation statements)

References 52 publications

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“…Figure 3 shows the schematic for detection probe development. Further, AuNPs have been utilized by Zhang et al in the development of AuNPs modified SBA-15 (Au@SBA-15) based detection probe for carbohydrate antigen 19-9 (CA 19-9) estimation [ 74 ]. Ab2-HRP was conjugated onto Au@SBA-15 via Au–NH 3+ or Au–SH affinity and enhanced direct electron transfer (DET) was utilized for signal enhancement.…”

Section: Electrochemical Elisa Based Detectionmentioning

confidence: 99%

Recent Advances in Enhancement Strategies for Electrochemical ELISA-Based Immunoassays for Cancer Biomarker Detection

Arya

Estrela

2018

Sensors

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Electrochemical enzyme-linked immunosorbent assay (ELISA)-based immunoassays for cancer biomarker detection have recently attracted much interest owing to their higher sensitivity, amplification of signal, ease of handling, potential for automation and combination with miniaturized analytical systems, low cost and comparative simplicity for mass production. Their developments have considerably improved the sensitivity required for detection of low concentrations of cancer biomarkers present in bodily fluids in the early stages of the disease. Recently, various attempts have been made in their development and several methods and processes have been described for their development, amplification strategies and testing. The present review mainly focuses on the development of ELISA-based electrochemical immunosensors that may be utilized for cancer diagnosis, prognosis and therapy monitoring. Various fabrication methods and signal enhancement strategies utilized during the last few years for the development of ELISA-based electrochemical immunosensors are described.

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Section: Electrochemical Elisa Based Detectionmentioning

confidence: 99%

Recent Advances in Enhancement Strategies for Electrochemical ELISA-Based Immunoassays for Cancer Biomarker Detection

Arya

Estrela

2018

Sensors

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“…Usually the performance of an amperometric sensor is heavily dependent on the electrode properties. The sensitivity of this type of sensor can go down to fM concentration of the analytes [20][21][22][23].…”

Section: Electrochemicalmentioning

confidence: 99%

The Emergence of Interdisciplinary Research in Cancer Diagnostics

Bellah¹

2017

JNMR

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“…Although this high-throughput biosensor is accurate and sensitive for the detection of ULBP2, with a limit of detection (LOD) of about 16 pg/mL, its signal detection method uses an expensive and sophisticated optical laser, so it cannot be used for PC screening at home. Aside from the ULBP2 biosensors, sandwich-type electrochemical immunosensors have also been reported for the detection of the CA 19-9 antigen [ 25 , 26 ]. Table 1 summarizes the performances of some electrochemical biosensors used for the detection of PC biomarkers.…”

Section: Introductionmentioning

confidence: 99%

An Immunosensor for the Detection of ULBP2 Biomarker

et al. 2020

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Pancreatic cancer (PC) is a global health problem that features a very high mortality rate. The UL16 binding protein 2 (ULBP2) is a new biomarker for PC detection. This study develops a simple, reliable, and inexpensive immunosensor for the detection of the ULBP2 antigen while also investigating the effects of an array configuration of connected sensors and zinc oxide (ZnO) nanoparticles on the immunosensor’s sensitivity. The ULBP2 antibody was immobilized onto the screen-printed carbon electrode (SPCE) surfaces of three different sensors: a simple SPCE (ULBP2-SPCE); an SPCE array, which is a series of identical SPCE connected to each other at different arrangements of rows and columns (ULBP2-SPCE-1x2 and ULBP2-SPCE-1x3); and an SPCE combined with ZnO nanoparticles (ULBP2-ZnO/SPCE). Impedance spectrum measurements for the immunosensors to ULBP2 antigen were conducted and compared. According to the result, the array configurations (ULBP2-SPCE-1x2 and ULBP2-SPCE-1x3) show an improvement of sensitivity compared to the ULBP2-SPCE alone, but the improvement is not as significant as that of the ULBP2-ZnO/SPCE configuration (ULBP2-ZnO/SPCE > ULBP2-SPCE: 18 times larger). The ULBP2-ZnO/SPCE immunosensor has a low limit of detection (1 pg/mL) and a high sensitivity (332.2 Ω/Log(pg/mL)), excellent linearity (R2 = 0.98), good repeatability (coefficients of variation = 5.03%), and is stable in long-term storage (retaining 95% activity after 28 days storage). In an array configuration, the immunosensor has an increased signal-to-noise ratio (ULBP2-SPCE-1x3 > ULBP2-SPCE: 1.5-fold) and sensitivity (ULBP2-SPCE-1x3 > ULBP2-SPCE: 2.6-fold). In conclusion, either the modification with ZnO nanoparticles onto the sensor or the use of an array configuration of sensors can enhance the immunosensor’s sensitivity. In this study, the best immunosensor for detecting ULBP2 antigens is the ULBP2-ZnO/SPCE immunosensor.

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Amperometric carbohydrate antigen 19-9 immunosensor based on three dimensional ordered macroporous magnetic Au film coupling direct electrochemistry of horseradish peroxidase

Cited by 34 publications

References 52 publications

Recent Advances in Enhancement Strategies for Electrochemical ELISA-Based Immunoassays for Cancer Biomarker Detection

Recent Advances in Enhancement Strategies for Electrochemical ELISA-Based Immunoassays for Cancer Biomarker Detection

The Emergence of Interdisciplinary Research in Cancer Diagnostics

An Immunosensor for the Detection of ULBP2 Biomarker

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