Amperometric enzyme electrode for the determination of aspartate aminotransferase and alanine aminotransferase in serum

Cooper, Jonathan M.; McNeil, Calum J.; Spoors, Julia A.

doi:10.1016/s0003-2670(00)80201-9

Cited by 34 publications

(30 citation statements)

References 18 publications

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“…Transaminase activity measured in 80 sera correlated well with results obtained with a spectrophotometric procedure. Cooper et al [72] demonstrated platinized activated carbon electrode immobilized by glutamate oxidase and the enzyme electrodes for the amperometric determination of l -glutamate in a stirred aqueous solution by the electrochemical detection of enzymically produced hydrogen peroxide at +320 mV vs. Ag/AgCl. Electrochemical experiments were done in a two-electrode stirred cell which contained a 5-mm diameter compacted Ag/AgCl combined reference and auxiliary electrode which surrounded a 1.5-mm diameter recessed gold contact in which the platinized carbon working electrode was placed.…”

Section: Electrochemical Techniquesmentioning

confidence: 99%

Aspartate Aminotransferase (AST/GOT) and Alanine Aminotransferase (ALT/GPT) Detection Techniques

Huang

Choi

et al. 2006

Sensors

418

212

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Abstract:The levels of aspartate aminotransferase (AST/GOT) and alanine aminotransferase (ALT/GPT) in serum can help people diagnose body tissues especially the heart and the liver are injured or not. This article provides a comprehensive review of research activities that concentrate on AST/GOT and ALT/GPT detection techniques due to their clinical importance. The detection techniques include colorimetric, spectrophotometric, chemiluminescence, chromatography, fluorescence and UV absorbance, radiochemical, and electrochemical techniques. We devote the most attention on experimental principle. In some methods a few representative devices and important conclusions are presented.

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Section: Electrochemical Techniquesmentioning

confidence: 99%

Aspartate Aminotransferase (AST/GOT) and Alanine Aminotransferase (ALT/GPT) Detection Techniques

Huang

Choi

et al. 2006

Sensors

418

212

View full text Add to dashboard Cite

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“…However, spectrophotometry requires a relatively large amount of expensive reagents, including enzymes and cofactors, and the procedures are complicated. In order to solve the problem, alternative methods based on electrochemical principles have been proposed (Mizutani et al, 1980(Mizutani et al, , 1998Kihara et al, 1984aKihara et al, , 1984bPeguin et al, 1989;Wollenberger et al, 1989;Cooper et al, 1991;Compagnone et al, 1992;Moser et al, 1997). Among them, detection schemes based on the production of l-glutamate as one of the final products are advantageous because l-glutamate can be measured amperometrically without additional cofactors (Cooper et al, 1991;Compagnone et al, 1992;Moser et al, 1997;Mizutani et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

Determination of the activities of glutamic oxaloacetic transaminase and glutamic pyruvic transaminase in a microfluidic system

Ohgami¹,

Upadhyay²,

Kabata³

et al. 2007

Biosensors and Bioelectronics

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“…The determination of transaminase activity on an amperometric sensor usually requires a rather long time (Wollenberger et al 1989;Compagnone et al 1992;Cooper et al 1991), owing to the low AST activity in the serum sample (Hart et al 1999). For achieving a rapid and highly sensitive measurement, the following approachs are taken into consideration.…”

Section: Introductionmentioning

confidence: 99%

Effect of L-Aspartate Concentration on the Response of the Amperometric L-Glutamate Sensor for the Measurement of L-Glutamate and Aspartate Aminotransferase Activity in Serum

Chang

Chen

2007

LANL

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L-glutamate oxidase was immobilized in a photo-cross-linkable polymer membrane on a palladium strip electrode for the amperometric measurement of aspartate aminotransferas eactivity. The sample, serum for example, was injected into a buffered L-aspartate and a-ketoglutarate solution. L-aspartate is the essential substrate and can transfer to L-glutamate via the aspartate aminotransferase catalyzing reaction. Aspartate aminotransferase activity can be measured by determining the increasing rate of L-glutamate. Under the optimal condition, the current increasing rate was proportional to the aspartate aminotransferase activity of the sample in the range of 8 -200 U/L. The data are in good correlation (R 2 ¼ 0.998) with data from 933 a commercial aspartate aminotransferase assay kit. Good reproducibility (relative standard deviation ¼ 3.03%, n ¼ 8) was obtained from a sample with 50 U/L aspartate aminotransferase activity. The sensor is expectable to be applied in a clinical point-of-care diagnosis.

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Amperometric enzyme electrode for the determination of aspartate aminotransferase and alanine aminotransferase in serum

Cited by 34 publications

References 18 publications

Aspartate Aminotransferase (AST/GOT) and Alanine Aminotransferase (ALT/GPT) Detection Techniques

Aspartate Aminotransferase (AST/GOT) and Alanine Aminotransferase (ALT/GPT) Detection Techniques

Determination of the activities of glutamic oxaloacetic transaminase and glutamic pyruvic transaminase in a microfluidic system

Effect of L-Aspartate Concentration on the Response of the Amperometric L-Glutamate Sensor for the Measurement of L-Glutamate and Aspartate Aminotransferase Activity in Serum

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