Amperometric enzyme immunosensor for the determination of the antigen of the pathogenic fungi Trichophyton rubrum

Medyantseva, É. P.; Халдеева, Е. В.; Glushko, N. I.; Budnikov, H. C.

doi:10.1016/s0003-2670(99)00889-2

Cited by 22 publications

(7 citation statements)

References 16 publications

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“…The recording of a loss in resonance frequency enabled the detection of 1 × 10 6 CFU/cm -3 . Medyantseva and colleagues [ 123 ] targeted an antigenic determinant on Trichophyton rubrum with a polyclonal antiserum. Their amperometric immunoassay had a sensitivity of 1 × 10 -15 mg/mL of antigen and was rapid (20 minutes).…”

Section: Immunosensors For Fungal Pathogens and Mycotoxinsmentioning

confidence: 99%

Antibody-Based Sensors: Principles, Problems and Potential for Detection of Pathogens and Associated Toxins

Byrne

Stack

Gilmartin

et al. 2009

Sensors

340

193

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Antibody-based sensors permit the rapid and sensitive analysis of a range of pathogens and associated toxins. A critical assessment of the implementation of such formats is provided, with reference to their principles, problems and potential for ‘on-site’ analysis. Particular emphasis is placed on the detection of foodborne bacterial pathogens, such as Escherichia coli and Listeria monocytogenes, and additional examples relating to the monitoring of fungal pathogens, viruses, mycotoxins, marine toxins and parasites are also provided.

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Section: Immunosensors For Fungal Pathogens and Mycotoxinsmentioning

confidence: 99%

Antibody-Based Sensors: Principles, Problems and Potential for Detection of Pathogens and Associated Toxins

Byrne

Stack

Gilmartin

et al. 2009

Sensors

340

193

View full text Add to dashboard Cite

show abstract

“…Specifically, by labeling an electroactive substance or enzyme on an antigen or antibody, the concentration of the antigen or antibody in the sample can be indirectly measured by monitoring the current change caused the reaction of the substrate of the electroactive substance or the enzyme catalyzed reaction between the immobilized biomolecule and the biomolecule in the solution. In this mode, the resulting current signal of the antigen antibody binding reaction has been amplified using the markers such as enzymes (alkaline phosphatase [ 27 , 28 ], horseradish peroxidase (HRP) [ 29 , 30 ] or glucose oxidase [ 31 , 32 ]) or nanomaterials (Au nanoparticles (AuNPs) [ 33 ], graphene [ 34 ], carbon nanotubes (CNTs) [ 35 , 36 ] or silica nanoparticles [ 37 , 38 ]), which has allowed the detection sensitivity and reproducibility to be increased to a certain extent. Additionally, according to the enzyme-linked immunoassay (ELISA) principle, this kind of immunosensors can be further divided into two categories based on competition or sandwich methodologies ( Figure 1 ) [ 39 ].…”

Section: Electrochemical Immunosensors With Different Signalsmentioning

confidence: 99%

Nanomaterials for Electrochemical Immunosensing

Pan

Yun

et al. 2017

Sensors

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Electrochemical immunosensors resulting from a combination of the traditional immunoassay approach with modern biosensors and electrochemical analysis constitute a current research hotspot. They exhibit both the high selectivity characteristics of immunoassays and the high sensitivity of electrochemical analysis, along with other merits such as small volume, convenience, low cost, simple preparation, and real-time on-line detection, and have been widely used in the fields of environmental monitoring, medical clinical trials and food analysis. Notably, the rapid development of nanotechnology and the wide application of nanomaterials have provided new opportunities for the development of high-performance electrochemical immunosensors. Various nanomaterials with different properties can effectively solve issues such as the immobilization of biological recognition molecules, enrichment and concentration of trace analytes, and signal detection and amplification to further enhance the stability and sensitivity of the electrochemical immunoassay procedure. This review introduces the working principles and development of electrochemical immunosensors based on different signals, along with new achievements and progress related to electrochemical immunosensors in various fields. The importance of various types of nanomaterials for improving the performance of electrochemical immunosensor is also reviewed to provide a theoretical basis and guidance for the further development and application of nanomaterials in electrochemical immunosensors.

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“…Н. И. Глушко 1 , Е. В. Халдеева 1 , Т. Г. Маланичева 2 , Е. В.Файзуллина 2 1 Казанский научно-исследовательский институт микробиологии и эпидемиологии; 2 Казанский государственный медицинский университет, Казань [5,6]. Трансдьюсером ИФС служил стационар ный ртутно-пленочный электрод с серебряной под ложкой.…”

Section: диагностические возможности иммуноферментного сенсора при кандидозахunclassified

Dendritic cell vaccines in immunotherapy of cancer

Moskaliova¹,

Смолякова²,

Жаворонок³

et al. 2017

Immunopathol Allergol Infectol

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The article is devoted to immunotherapy of tumors with dendritic cell vaccines. The history of the discovery of vaccines based on dendritic cells, the principles of creation and use, advantages and disadvantages of their use for therapeutic purposes compared with other types of anticancer vaccines, indications and contraindications are described. Clinical efficacy of immunotherapy of tumors of different localizations with dendritic cell vaccines is analyzed. Information about the number of clinical trials held in the world on the basis of immunotherapy with dendritic cell vaccines is provided. The results of our own studies of immune status of 6 women diagnosed with breast cancer prior to treatment are рrovided.

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Amperometric enzyme immunosensor for the determination of the antigen of the pathogenic fungi Trichophyton rubrum

Cited by 22 publications

References 16 publications

Antibody-Based Sensors: Principles, Problems and Potential for Detection of Pathogens and Associated Toxins

Antibody-Based Sensors: Principles, Problems and Potential for Detection of Pathogens and Associated Toxins

Nanomaterials for Electrochemical Immunosensing

Dendritic cell vaccines in immunotherapy of cancer

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