Amperometric l-lysine determination biosensor amplified with l-lysine oxidase nanoparticles and graphene oxide nanoparticles

Nohwal, Bhawna; Chaudhary, Reeti; Pundir, C.S.

doi:10.1016/j.procbio.2020.06.011

Cited by 18 publications

(3 citation statements)

References 32 publications

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“…Currently, electrochemical biosensors are applied for highly sensitive determination of different classes of viruses, including human coronavirus, HIV, HBV, HCV, and dengue virus. These biosensors (i.e., potentiometric [ 109 ], amperometric [ 110 – 112 ], and conductometric [ 113 ]) play significant roles in various biosensensing fields because of their facile fabrication, small size, facile interpret results, robustness, and low detection and quantification limits. In potentiometric biosensors, a bioreceptor and a transducer are combined for detecting changes in the concentration of ions, and consequently, in these biosensors, the obtained analytical response reflects the concentration of the target analyte.…”

Section: Detection Methodsmentioning

confidence: 99%

An Overview on SARS-CoV-2 (COVID-19) and Other Human Coronaviruses and Their Detection Capability via Amplification Assay, Chemical Sensing, Biosensing, Immunosensing, and Clinical Assays

et al. 2020

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A novel coronavirus of zoonotic origin (SARS-CoV-2) has recently been recognized in patients with acute respiratory disease. COVID-19 causative agent is structurally and genetically similar to SARS and bat SARS-like coronaviruses. The drastic increase in the number of coronavirus and its genome sequence have given us an unprecedented opportunity to perform bioinformatics and genomics analysis on this class of viruses. Clinical tests like PCR and ELISA for rapid detection of this virus are urgently needed for early identification of infected patients. However, these techniques are expensive and not readily available for point-of-care (POC) applications. Currently, lack of any rapid, available, and reliable POC detection method gives rise to the progression of COVID-19 as a horrible global problem. To solve the negative features of clinical investigation, we provide a brief introduction of the general features of coronaviruses and describe various amplification assays, sensing, biosensing, immunosensing, and aptasensing for the determination of various groups of coronaviruses applied as a template for the detection of SARS-CoV-2. All sensing and biosensing techniques developed for the determination of various classes of coronaviruses are useful to recognize the newly immerged coronavirus, i.e., SARS-CoV-2. Also, the introduction of sensing and biosensing methods sheds light on the way of designing a proper screening system to detect the virus at the early stage of infection to tranquilize the speed and vastity of spreading. Among other approaches investigated among molecular approaches and PCR or recognition of viral diseases, LAMP-based methods and LFAs are of great importance for their numerous benefits, which can be helpful to design a universal platform for detection of future emerging pathogenic viruses.

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Section: Detection Methodsmentioning

confidence: 99%

An Overview on SARS-CoV-2 (COVID-19) and Other Human Coronaviruses and Their Detection Capability via Amplification Assay, Chemical Sensing, Biosensing, Immunosensing, and Clinical Assays

et al. 2020

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“…The presence of CD59 was further explored in the first-morning urine samples of lung cancer patients, comparing it with samples from apparently healthy adults using the immunosensor. Moreover, the non-invasive immunosensor demonstrated good storage stability and specificity, underscoring its potential for practical applications [74].…”

Section: Using and Application Of Nanoparticles On Non-invasive Elect...mentioning

confidence: 98%

The role of nanoparticles in non‐invasive electrochemical immunosensor technology and recent developments

Sanko,

Erkmen,

Kuralay

2024

Electroanalysis

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“…By selecting the appropriate applied voltage and electrode material, the amperometric method enables the selective separation of various electroactive compounds in solution [12]. The amperometric biosensor method has previously been used by Nohwal [13], which has good sensitivity and reproducibility with a fast response time for L-lysine detection but is expensive and complicated, and there is interference from other substances.…”

Section: Amperometric Measurementmentioning

confidence: 99%

Detection of L-lysine in food supplement based on amperometric biosensors

Umar,

Ibmar,

Rosandi

et al. 2023

J. Phys.: Conf. Ser.

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L-lysine is an essential amino acid not synthesized by the body and is used as a nutritional supplement, and is considered an index of the nutritional quality of foodstuffs. The level of L-lysine consumption depends on age, where an imbalance in L-lysine levels can cause certain disease conditions. This study identified the effects of L-lysine contained in food supplements using the yeast-based amperometric biosensor Saccharomyces cerevisiae, which measures dissolved oxygen (DO) levels due to cellular respiration of yeast with an analyte assay sample. The measurements were made by calibrating pure L-lysine at 100, 200 and 300 mM concentrations. The results show that the higher the concentration of L-lysine, the smaller the level of DO remaining detected, where the percentage range of DO is 13.47% - 26.02%. The dietary supplement sample test, namely Curcuma Plus, has an error of 1.01% which states that the biosensor used can detect L-lysine levels in samples with high sensitivity and selectivity and can be applied to the food and health industries. The measurement results are clustered using Principal Component Analysis (PCA) to see the resulting clusters and data patterns.

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Amperometric l-lysine determination biosensor amplified with l-lysine oxidase nanoparticles and graphene oxide nanoparticles

Cited by 18 publications

References 32 publications

An Overview on SARS-CoV-2 (COVID-19) and Other Human Coronaviruses and Their Detection Capability via Amplification Assay, Chemical Sensing, Biosensing, Immunosensing, and Clinical Assays

An Overview on SARS-CoV-2 (COVID-19) and Other Human Coronaviruses and Their Detection Capability via Amplification Assay, Chemical Sensing, Biosensing, Immunosensing, and Clinical Assays

The role of nanoparticles in non‐invasive electrochemical immunosensor technology and recent developments

Detection of L-lysine in food supplement based on amperometric biosensors

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