Amperometric sensing system for the detection of urea by a combination of the pH-stat method and flow injection analysis

Yoneyama, Kenji; Fujino, Yusuke; Osaka, Tetsuya; Satoh, Isao

doi:10.1016/s0925-4005(01)00613-x

Cited by 30 publications

(17 citation statements)

References 19 publications

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“…A group from the University of Twente has characterized systems consisting of a pH-ISFET and an actuator electrode to cause the electrolysis of water [95][96][97]. Similar systems have also been reported by other groups [98][99][100]. Moreover, with the progress of microfluidc technologies, situations are also increasing in which the control of pH is a critical function.…”

Section: Automatic Regulation Of the Ph Of A Solutionmentioning

confidence: 63%

Electrochemical techniques for microfluidic applications

et al. 2008

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Electrochemical principles provide key techniques to promote the construction of bio/chemical microsystems of the next generation. There is a wealth of technology for the microfabrication of bio/chemical sensors. In addition, microfluidic transport in a network of flow channels, pH regulation, and automatic switching can be realized by electrochemical principles. Since the basic components of the devices are electrode patterns, the integration of different components is easily achieved. With these techniques, bio/chemical assays that require the exchange of solutions can be conducted on a chip. Furthermore, autonomous microanalysis systems that can carry out necessary procedures are beginning to be realized. In this article, techniques developed in our group will be comprehensively introduced.

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Section: Automatic Regulation Of the Ph Of A Solutionmentioning

confidence: 63%

Electrochemical techniques for microfluidic applications

et al. 2008

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“…Total lipid concentration (mg dL −1 ) was estimate by method of Vatassery et al (1981). In addition, kidney function was assessed by measuring creatinine concentration (mg dL −1 ) according to Demirovic et al (2009), uric acid concentration (mg dL −1 ) by the method of Carolina et al (2005) also urea concentration (mmol L −1 ) was estimated according to Yoneyame et al (2001).…”

Section: Biochemical Assessment Of Sub-chronic Toxicity Studymentioning

confidence: 99%

Production and Characterization of Exopolysaccharide from Novel <i>Bacillus</i> sp. M3 and Evaluation on Development Sub-Chronic Aluminum Toxicity Induced Alzheimerâs Disease in Male Rats

Asker

Ibrahim

Mahmoud

et al. 2015

American Journal of Biochemistry and Biotechnology

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Abstract:The number of patients suffering from Alzheimer's Disease (AD) all over the world is rising continually and becomes one of the biggest challenges for most societies throughout the world. The potential of peripheral biochemical markers as complementary tools in the neuronphsychiatric evaluation of these patients has claimed further attention. The aims of our study were to isolate the bacteria that able to produce exo-polysaccharides and to characterize then exopolysaccharide producing strains by 16S rDNA sequencing method. The exopolysaccharide (MEPS) produced from a newly isolated Bacillus sp. M3 was obtained by ethanol precipitation (6.5 g L −1 growth medium). The molecular masse of the MEPS was 1.45×10 4 g/mol wherein FT-IR, UVVis spectral analyses revealed prevalence of characteristic primary belonged to α-type exopolysaccharide with a pyran ring. Further, HPLC analysis revealed its two monosaccharide constituents galacturonic acid and glucuronic acid at molar ratio of 1:1. The second stage was the evaluation of anti-intoxicated effect of MEPS against aluminum chloride induced Alzheimer in rat relevant to its effect on oxidative stress, antioxidant brain status, cholinergic markers and serum level of S100B protein together with polysaccharide sub-chronic toxicity at 1/10 LD 50 in both AD and control experimental animal groups to find their diagnostic value in this disease. MEPS ameliorated antioxidant status and reduced all oxidative stress parameters in brain tissue with decreasing S100B as compared to aluminum toxicant group with significant acetyl cholinesterase inhibition which increase acetylcholine concentration in brain tissue. No toxicity was observed for MEPS in sub-chronic toxicity study for 90 days in all rat organs or liver and kidney function.

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“…These sensors are affected by the impedance because of alkali and pH changes. Then again, moderately few amperometric urea sensors in view of recognition of alkali have been accounted for [12][13][14][15]. Numerous amperometric biosensors of urea in view of immobilization of urease in directing polymers have likewise been created [16][17][18][19][20][21][22][23].…”

Section: Introductionmentioning

confidence: 99%

Synthesis of Polyaniline Based Nanocomposite Graphite Paste Electrode Modified with and Without Rice Husk for Sensor Application: A Comparative Study

Paithankar¹,

Deshmukh²,

Shelke³

et al. 2017

Asian J. Chem.

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Polyaniline based nanocomposite graphite paste electrode modified with rice husk (PANI/Gr/RH) and without rice husk (PANI/Grph) to compare the amperometric response caused by the addition of rice husk in conventional sensor. Organized materials were characterized by analytical techniques such as UV-visible, XRD and FE-SEM analyses. The performance of the developed sensor was evaluated and the obtained urea biosensor exhibited shorter response time (3 s), wider range 1 × 10 -9 to 9 × 10 -9 M and the detection limit were found to be 1 × 10 -9. About 90 % of the enzyme activity is retained for about 40 days. Modified rice husk sensor gives better result than sensor without rice husk.

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Amperometric sensing system for the detection of urea by a combination of the pH-stat method and flow injection analysis

Cited by 30 publications

References 19 publications

Electrochemical techniques for microfluidic applications

Electrochemical techniques for microfluidic applications

Production and Characterization of Exopolysaccharide from Novel <i>Bacillus</i> sp. M3 and Evaluation on Development Sub-Chronic Aluminum Toxicity Induced Alzheimerâs Disease in Male Rats

Synthesis of Polyaniline Based Nanocomposite Graphite Paste Electrode Modified with and Without Rice Husk for Sensor Application: A Comparative Study

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Amperometric sensing system for the detection of urea by a combination of the pH-stat method and flow injection analysis

Cited by 30 publications

References 19 publications

Electrochemical techniques for microfluidic applications

Electrochemical techniques for microfluidic applications

Production and Characterization of Exopolysaccharide from Novel <i>Bacillus</i> sp. M3 and Evaluation on Development Sub-Chronic Aluminum Toxicity Induced Alzheimerâs Disease in Male Rats

Synthesis of Polyaniline Based Nanocomposite Graphite Paste Electrode Modified with and Without Rice Husk for Sensor Application: A Comparative Study

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Production and Characterization of Exopolysaccharide from Novel <i>Bacillus</i> sp. M3 and Evaluation on Development Sub-Chronic Aluminum Toxicity Induced Alzheimerâs Disease in Male Rats