Amperometric thin film biosensors based on glucose dehydrogenase and Toluidine blue O as catalyst for NADH electrooxidation

Boguslavsky, L.I.; Geng, Lin; Kovalev, I. P.; Sahni, Suresh K.; Xu, Zhiai; Skotheim, Terje A.; Laurinavičius, Valdas; Persson, Björn; Gorton, Lo

doi:10.1016/0956-5663(95)96960-7

Cited by 40 publications

(25 citation statements)

References 22 publications

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“…These are NAD-dependent GDH 40 and FAD-dependent GDH. 41 These enzymes combine the oxygen independence of PQQ-GDH with the specificity (toward nonglucose sugars) of GOx, and it is likely that they will be more widely used in the future.…”

Section: The Enzymes Of Glucose Electrooxidizing Anodesmentioning

confidence: 99%

Electrochemical Glucose Sensors and Their Applications in Diabetes Management

2008

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About 6,000 peer reviewed articles have been published on electrochemical glucose assays and sensors, of which 700 were published in the 2005-2006 two-year period. Their number makes a full review of the literature, or even of the most recent advances, impossible. Nevertheless, this review should acquaint the reader with the fundamentals of the electrochemistry of glucose and provide a perspective of the evolution of the electrochemical glucose assays and monitors helping diabetic people, who constitute about 5% of the world's population. Because of the large number of diabetic people, no assay is performed more frequently than that of glucose. Most of these assays are electrochemical. The reader interested also in nonelectrochemical assays used in, or proposed for, the management of diabetes is referred to a 2007 review of Kondepati and Heise. 1 Adam Heller was born in 1933. Surviving the Holocaust, he arrived in Israel in 1945. He received his M.Sc. in Chemistry and Physics in 1957, then his PhD in Organic Chemistry in 1961 from Ernst David Bergman at the Hebrew University in Jerusalem. He postdoced at UC Berkeley (1962-3) and at Bell Laboratories . At GTE Labs (1964-1975 he built the first Nd 3+ liquid laser and, with J. J. Auborn, the still worldwide used Li/SOCl 2 battery. At Bell Labs (1975)(1976)(1977)(1978)(1979)(1980)(1981)(1982)(1983)(1984)(1985)(1986)(1987)(1988) he designed the first >10% efficient electrochemical solar cells and the first >10% efficient hydrogengenerating solar-powered photoelectrode. He also headed Bell Labs' Electronic Materials Research Department (1977)(1978)(1979)(1980)(1981)(1982)(1983)(1984)(1985)(1986)(1987)(1988), which developed part of the high density chip interconnection technology underlying the miniaturization of portable electronic devices. He was appointed to the Ernest Cockrell Sr. Chair in Engineering of the University of Texas at Austin in 1988, and in 2001 became one of UT's first Research Professors. At UT he pioneered the electrical wiring of enzymes. In 1996 he cofounded with his son Ephraim Heller TheraSense Inc., now part of Abbott Diabetes Care, to improve the lives of diabetic people. The company introduced in 2000 the blood sugar monitor FreeStyle, a thin-layer microcoulometer utilizing only 300 nL of blood, so little that it was, for the first time, painlessly obtained. In 2007 it provided for more than 1 billion painless glucose assays. After alleviating the pain of diabetes monitoring, FreeStyle Navigator, based on the electrical wiring of glucose oxidase, introduced in 2007 in Europe and Israel and in 2008 in the U.S., removed the worry of diabetic people by continuously monitoring their glucose levels. Heller aims his work at alleviating suffering through bioelectrochemistry.

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Section: The Enzymes Of Glucose Electrooxidizing Anodesmentioning

confidence: 99%

Electrochemical Glucose Sensors and Their Applications in Diabetes Management

2008

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“…35 It is easily electrodeposited on the surface of glassy carbon electrode by electropolymerization. 31,32 In the present study, a poly(toluidine blue) modified electrode showed excellent electrocatalytic activity toward DA and AA, and possessed a remarkable property of molecular recognition toward DA and AA, which has not been reported elsewhere. This resulting modified electrode is easier to be miniaturized (compared with a Cu-dispersed sol-gel modified electrode), 30 and possess the analytic characteristics of a wider linear detection range for the detection of DA and AA (compared with a polyphenosafranine modified electrode) 23 and of a lower detection limit of DA (by a comparison with N,N-dimethylaniline).…”

mentioning

confidence: 84%

“…Toluidine Blue, a phenothiazine dye, has been extensively studied concerning on the electrocatalysis of some bioactive compounds, such as the reductive nicotinamide adenine dinucleotide (NADH), [31][32][33] hemoprotein 34 and myoglobin. 35 It is easily electrodeposited on the surface of glassy carbon electrode by electropolymerization.…”

mentioning

confidence: 99%

Simultaneous Determination of Dopamine and Ascorbic Acid at a Poly(Toluidine Blue) Modified Electrode

et al. 2004

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“…[18][19][20] Our subsequent goal was to extend amperometric biosensors based on the PPF technique to GDH-based devices. Two kinds of GDH have been reported: one is NAD(P)-dependent [6][7][8][9][10][11][12] and the other is pyrroloquinoline quinone (PQQ)-dependent. [13][14][15][16] In this research, we chose NAD-dependent as a model of GDH family and here we report the usefulness and effectiveness of PPFs in GDH amperometric biosensors.…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, reports of GDH-based amperometric biosensors have been increasing. [6][7][8][9][10][11][12][13][14][15][16] A plasma-polymerized film (PPF) is synthesized by a glow discharge or plasma in the vapor phase. 17 The properties of the PPF include (a) the ability to be made extremely thin (<1 μm), (b) good adhesion to any substrate, (c) pinhole-free and flat surface structure, (d) mechanical and chemical stability (a result of its highly-branched and cross-linked structure), and (e) the capability to accommodate a large amount of biomolecules, such as enzymes, antibodies, and oligonucleotides, which can be immobilized onto the surface of the film.…”

Section: Introductionmentioning

confidence: 99%