Amplification of microsphere-based microarrays using catalyzed reporter deposition

Anderson, George P.; Taitt, Chris R.

doi:10.1016/j.bios.2008.03.045

Cited by 28 publications

(13 citation statements)

References 16 publications

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“…Fluorescent sandwich immunoassays have been also performed on beads with flow cytometry instrumentation used to detect and quantify toxin [77,78,79]. Detection of BoNT/A and BoNT/B was performed in multiplex with other toxins (e.g., cholera toxin, ricin and staphylococcal enterotoxin B (SEB)) using different color-coded beads [78,79].…”

Section: Flow Cytometric Assaysmentioning

confidence: 99%

“…Detection of BoNT/A and BoNT/B was performed in multiplex with other toxins (e.g., cholera toxin, ricin and staphylococcal enterotoxin B (SEB)) using different color-coded beads [78,79]. The use of magnetic beads in flow cytometry was shown to have several advantages including that a preconcentration step can be involved, resulting in increased sensitivity and making analysis of turbid or heterogenous samples possible as beads can be easily separated from the matrix [79].…”

Section: Flow Cytometric Assaysmentioning

confidence: 99%

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Sensing the Deadliest Toxin: Technologies for Botulinum Neurotoxin Detection

Čapek

Dickerson

2010

Toxins

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Sensitive and rapid detection of botulinum neurotoxins (BoNTs), the most poisonous substances known to date, is essential for studies of medical applications of BoNTs and detection of poisoned food, as well as for response to potential bioterrorist threats. Currently, the most common method of BoNT detection is the mouse bioassay. While this assay is sensitive, it is slow, quite expensive, has limited throughput and requires sacrificing animals. Herein, we discuss and compare recently developed alternative in vitro detection methods and assess their ability to supplement or replace the mouse bioassay in the analysis of complex matrix samples.

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Section: Flow Cytometric Assaysmentioning

confidence: 99%

Section: Flow Cytometric Assaysmentioning

confidence: 99%

Sensing the Deadliest Toxin: Technologies for Botulinum Neurotoxin Detection

Čapek

Dickerson

2010

Toxins

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“…202–204 Moreover, various patterns for signal amplification have been developed to increase overall sensitivity. 205–209 …”

Section: Optical Label and Signal Amplificationmentioning

confidence: 99%

Suspension arrays based on nanoparticle-encoded microspheres for high-throughput multiplexed detection

et al. 2015

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Spectrometrically or optically encoded microsphere based suspension array technology (SAT) is applicable to the high-throughput, simultaneous detection of multiple analytes within a small, single sample volume. Thanks to the rapid development of nanotechnology, tremendous progress has been made in the multiplexed detecting capability, sensitivity, and photostability of suspension arrays. In this review, we first focus on the current stock of nanoparticle-based barcodes as well as the manufacturing technologies required for their production. We then move on to discuss all existing barcode-based bioanalysis patterns, including the various labels used in suspension arrays, label-free platforms, signal amplification methods, and fluorescence resonance energy transfer (FRET)-based platforms. We then introduce automatic platforms for suspension arrays that use superparamagnetic nanoparticle-based microspheres. Finally, we summarize the current challenges and their proposed solutions, which are centered on improving encoding capacities, alternative probe possibilities, nonspecificity suppression, directional immobilization, and “point of care” platforms. Throughout this review, we aim to provide a comprehensive guide for the design of suspension arrays, with the goal of improving their performance in areas such as multiplexing capacity, throughput, sensitivity, and cost effectiveness. We hope that our summary on the state-of-the-art development of these arrays, our commentary on future challenges, and some proposed avenues for further advances will help drive the development of suspension array technology and its related fields.

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“…In this context, flow cytometry has been shown to be a useful technique to detect and to quantify bead-bound BoNT molecules through a fluorescent readout (Anderson and Taitt 2008;Ozanich et al 2009;Warner et al 2009). A further development is suspension array technologies (e.g., the Luminex Ò xMAP technology).…”

Section: Selected Examples Of Different Elisa Formatsmentioning

confidence: 99%

Complexity of Botulinum Neurotoxins: Challenges for Detection Technology

Dorner

Schulz

Kull

et al. 2012

Current Topics in Microbiology and Immunology

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The detection of botulinum neurotoxins (BoNT) is extremely challenging due to their high toxicity and the multiple BoNT variants. To date, seven serotypes with more than 30 subtypes have been described, and even more subtypes are expected to be discovered. The fact that the BoNT molecules are released as large complexes of different size and composition adds further complexity to the issue. Currently, in the diagnostics of botulism, the mouse bioassay (MBA) is still considered as gold standard for the detection of BoNT in complex sample materials. Over the years, different functional, immunological, and spectrometric assays or combinations thereof have been developed, supplemented by DNA-based assays for the detection of the organism. In this review, advantages and limitations of the current technologies will be discussed, highlighting some of the intricacies of real sample analysis.

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Amplification of microsphere-based microarrays using catalyzed reporter deposition

Cited by 28 publications

References 16 publications

Sensing the Deadliest Toxin: Technologies for Botulinum Neurotoxin Detection

Sensing the Deadliest Toxin: Technologies for Botulinum Neurotoxin Detection

Suspension arrays based on nanoparticle-encoded microspheres for high-throughput multiplexed detection

Complexity of Botulinum Neurotoxins: Challenges for Detection Technology

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