Amplification test and selection of markers analogue to resistance genes in species and commercial varieties of Passiflora spp.

Abstract

Brazil is highlighted as the largest producer and consumer of passion fruit (Passiflora) and holder of representative biodiversity. Studies dedicated to the genetic characterization of the genus in order to understand the existing diversity are important for the advancement of genetic improvement and conservation programs. The aim of this study was to select combinations of RGA (Resistance Genes Analogs) primers for commercial passion fruit species and varieties. To this end, amplification tests were performed on a PCR platform in 17 combinations of RGA primers for eight species and 12 commercial varieties of passion fruit from the Banco Ativo de Germoplasma Flor da Paixão. All combinations of primers have generated amplification products in at least one of the Passiflora samples. The number of combinations that generated amplification products varied from seven to 12 in the species and from three to eight in the varieties. Three to 12 combinations generated amplification products for the samples, emphasizing that the RGA markers were efficient in accessing genomic loci in the species and varieties of passion fruit. The variation of efficient combinations in each of the species and varieties attested to the importance of this research in the preliminary stages of genetic studies as auxiliary tools in conservation and genetic improvement programs. However, this study made it possible to identify combinations of primers to be prioritized in inter and intraspecific genetic characterizations of passion fruit.

Characterization and selection of markers associated with resistance analogous genes as input for genetic analysis of Prosopis juliflora (Sw.) DC.

“…These described adequate amplification with 100% polymorphism for Passiflora Spp. (Souza et al, 2020). In Passiflora setácea, they registered polymorphism ranging from 99 to 100% (Pereira, Corrêa, & Oliveira, 2015).…”

Molecular characterization of Passiflora edulis f. flavicarpa Degener with ISSRs markers

“…Therefore, cross-pollination mediated by external agents is required, which contributes to the increase of the genetic variability of these species, for example, in P. cincinnata (Silva et al, 2022). Sousa et al (2020) using 31 ISSR markers in 25 wild passion fruit species and Sousa et al (2015) observed that 20 primers had polymorphic loci. In view of the studies with the same primers in different species of the genus, it was observed that these markers, in addition to enabling molecular studies, also inform the divergence between species, whose characteristic makes this marker a potential tool for the selection of taxa in wild species of Passiflora L.…”

Variabilidade e estrutura genética molecular em acessos de Passiflora edulis Sims. com base em marcadores análogos a genes de resistência

“…Para a caracterização da diversidade genética, foram utilizados nove pares de iniciadores RGA previamente selecionados para o gênero Passiflora (Tabela 1) (PAULA et al, 2010;SOUSA et al, 2015). A despeito de comporem a classe dos marcadores dominantes, cuja genotipagem não permite a distinção de alelos (ou seja, a identificação direta de heterozigotos), a heterozigosidade esperada (He) pode ser estimada de forma aproximada considerando a hipótese do EHW.…”

“…Também contribuem para a construção de mapas genéticos, juntamente com outros tipos de marcadores (PEREIRA et al, 2013). Além disso, estudos de avaliação com marcador molecular dessa natureza em diferentes espécies e variedades comerciais de maracujazeiros trazem informações de iniciadores mais adequados e informativos para a caracterização genética (SOUZA et al, 2020).…”