Amplified ribosomal DNA restriction analysis of free-living bacteria present in the headbox of a Canadian paper machine

Abstract

The headbox water is the main source of bacterial contamination of paper machines. Identification of these bacterial contaminants could be an asset in developing specific control methods. An amplified ribosomal DNA restriction analysis (ARDRA) was carried out to characterize the bacterial communities associated with the headbox water of a paper machine in a Canadian mill in February and July 2006. Eight bacterial genera were identified as the main colonizers present in the headbox water. The genus Meiothermus appeared to be the dominant bacterial group in the Canadian paper machine. Some variation was observed between the February and July clone libraries. Bacterial genera such as Chelatococcus and Hydrogenophilus were only detected in February or in July, respectively. Furthermore, the proportion of Tepidimonas clones in the libraries was higher in July than in February. The metabolic profile of the February and July communities, determined using Biolog EcoPlates, also suggested that temporal variation occurred within the bacterial populations that colonized the headbox of the paper machine.

The Family Beijerinckiaceae

“…In vivo, proteins of desiccation-resistant bacteria were protected from oxidative modifications that introduce carbonyl groups in sensitive bacteria during drying. Members of Chelatococcus have also been identified among other genera present in the headbox water of a paper machine in Canada (Prince et al 2009), using an amplified ribosomal DNA restriction analysis.…”

Pseudoxanthomonas bacteria that drive deposit formation of wood extractives can be flocculated by cationic polyelectrolytes

“…This bacterium has been shown prevalent in the water circuits of many pulp and paper mills [9,22,25,26]. We showed that the white water bacteria Psx.…”

“…Pseudoxanthomonas taiwanensis is a frequent and abundant contaminant of paper machines [9,22,31]. Abietic acid was used as the model resin acid because it is a major component in spruce wood pitch [15,32].…”