Amygdalin Regulates Apoptosis and Adhesion in Hs578T Triple-Negative Breast Cancer Cells

Lee, Hye Min; Moon, Aree

doi:10.4062/biomolther.2015.172

Cited by 83 publications

(44 citation statements)

References 34 publications

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“…Immunoblot analysis was performed as described previously (23). Protein extracts in lysis buffer [50 mM Tris, 2% sodium dodecyl sulfate (SDS), 1 mM ethylenediaminetetraacetic acid (EDTA) and 0.1 M dithiothreitol) containing protease inhibitor cocktail (Roche Diagnostics GmbH, Mannheim, Germany) were subjected to 12% SDS-polyacrylamide gel electrophoresis.…”

Section: Methodsmentioning

confidence: 99%

Farnesyl transferase inhibitor FTI-277 inhibits breast cell invasion and migration by blocking H-Ras activation

2016

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Abstract. Hyperactive Ras promotes proliferation and malignant phenotypic conversion of cells in cancer. Ras protein must be associated with cellular membranes for its oncogenic activities through post-translational modifications, including farnesylation. Farnesyltransferase (FTase) is essential for H-Ras membrane targeting, and H-Ras, but not N-Ras, has been demonstrated to cause an invasive phenotype in MCF10A breast epithelial cells. In the present study, it was observed that an FTase inhibitor (FTI), FTI-277, blocked epidermal growth factor (EGF)-induced H-Ras activation, but not N-Ras activation in MDA-MB-231 cells, which express wild-type H-Ras and N-Ras. FTI-277 exerted a more potent inhibitory effect on the proliferation of H-Ras-MCF10A cells and Hs578T breast cancer cells expressing an active mutant of H-Ras than that of MDA-MB-231 cells. The invasive/migratory phenotypes of the H-Ras-MCF10A and Hs578T cells were effectively inhibited by FTI-277 treatment. By contrast, FTI-277 did not affect the invasive/migratory phenotypes of MDA-MB-231 cells. However, the EGF-induced invasion of MDA-MB-231 cells was decreased by FTI-277, implicating that FTI-277 inhibits breast cell invasion and migration by blocking H-Ras activation. Taken together, the results of the present study suggest that FTase inhibition by FTI-277 may be an effective strategy for targeting H-Ras-mediated proliferation, migration and invasion of breast cells.

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Section: Methodsmentioning

confidence: 99%

Farnesyl transferase inhibitor FTI-277 inhibits breast cell invasion and migration by blocking H-Ras activation

2016

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“…Chang et al found that the expression of Bax and the caspase‐3 enzyme activity were increased while the expression of Bcl‐2 was decreased in DU145 and LNCaP cells after amygdalin treatment, leading to PCa cell apoptosis. Lee et al determined the expression levels of apoptosis‐related proteins in breast cancer cells, which treated with amygdalin at various concentrations, and found that amygdalin increased the expression of pro‐apoptotic protein Bax and decreased that of antiapoptotic Bcl‐2 and pro‐ Caspase‐3. At the same time, PARP cleavage was observed in breast cancer cells treated with amygdalin.…”

Section: The Basic Antitumor Mechanisms Of Amygdalinmentioning

confidence: 99%

Recent updates and future perspectives about amygdalin as a potential anticancer agent: A review

Shi

Chen

et al. 2019

Cancer Medicine

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The overall incidence of cancer is increasing in recent years. Despite advances in various comprehensive treatments, the mortality of advanced malignant tumors remains at a high level. Numerous pharmacological studies have confirmed that many Chinese herbal medicines possess remarkable antitumor activities. Amygdalin, mainly existing in bitter almond, is reported to have antitumor properties in addition to the antioxidative, antibacterial, anti‐inflammatory and immunoregulatory activities. This article summarizes the structural characteristics of amygdalin, its antitumor mechanisms, and recent progress and achievement in the research of amygdalin, hoping that it could provide theoretical clues for exploring the clinical value of amygdalin against tumors. Amygdalin is known to have an antitumor effect in solid tumors such as lung cancer, bladder cancer and renal cell carcinoma by affecting cell cycle, inducing apoptosis and cytotoxicity, and regulating immune function. Further research is needed to elucidate the pharmacological mechanisms of amygdalin in terms of the optimal dosage, the feasibility of combined use of amygdalin with other antitumor drugs, and even artificial synthesis of the active components in amygdalin, for the sake of enhancing its antitumor activities and reducing its adverse effects for clinical use.

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“…Amygdalin is a cyanogenic glucoside derived from seeds of rosaceous plants, for example seeds of bitter almonds (Prunus dulcis), or apricot, cherry, apple, peach, plum, etc. [4,5,6,7]. Amygdalin C 20 H 27 NO 11 , is composed of one molecule of benzaldehyde, two molecules of glucose and one molecule of hydrocyanic acid [8].…”

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confidence: 99%

Human Urine Alterations Caused by Apricot Seeds Consumption

Tušimová

Zbyňovská

Kováčik

et al. 2017

Advanced Research in Life Sciences

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Amygdalin is a cyanogenic glucoside derived from various plants seeds. It is a natural product that owns antitumor activity, it has also been used for the treatment of asthma, bronchitis, emphysema, leprosy and diabetes and produces antitussive and antiasthmatic effects. The present in vivo study was designed to reveal whether amygdalin in apricot seeds has got an effect on human urine composition, pH value and urine associated health status after twelve weeks of oral administration. The study group finally consisted of 12 healthy adult volunteers. All participants consumed 60 mg/kg of body weight of bitter apricot seeds daily (approximately 3.0 mg/kg of amygdalin) during 12 weeks. Two urine collections were carried out (at the beginning of the experiment and after 84 days). Quantification of urine calcium, magnesium, phosphorus, sodium, potassium, chlorides, urea and pH value after apricot seeds supplementation was performed. Paired t-test showed, that consumption of bitter apricot seeds during 84 days had a significant (P < 0.01) effect on amount of calcium excreted in urine, though this decrease shifted its level from elevated mean value in control collection into normal physiological range. No significant changes were observed in other parameters after apricot seed ingestion.

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Amygdalin Regulates Apoptosis and Adhesion in Hs578T Triple-Negative Breast Cancer Cells

Cited by 83 publications

References 34 publications

Farnesyl transferase inhibitor FTI-277 inhibits breast cell invasion and migration by blocking H-Ras activation

Farnesyl transferase inhibitor FTI-277 inhibits breast cell invasion and migration by blocking H-Ras activation

Recent updates and future perspectives about amygdalin as a potential anticancer agent: A review

Human Urine Alterations Caused by Apricot Seeds Consumption

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