Amyloid β-but not Tau-induced neurotoxicity is suppressed by Manuka honey<i>via</i>HSP-16.2 and SKN-1/Nrf2 pathways in an<i>in vivo</i>model of Alzheimer's disease

Navarro-Hortal, María D.; Romero-Márquez, Jose M.; Muñoz-Ollero, Pedro; Jiménez-Trigo, Victoria; Esteban-Muñoz, Adelaida; Tutusaus, Kilian; Giampieri, Francesca; Battino, Maurizio; Sánchez-González, Cristina; Rivas-García, Lorenzo; Llopis, Juan; Forbes‐Hernández, Tamara Y.; Quiles, José L.

doi:10.1039/d2fo01739c

Cited by 13 publications

(5 citation statements)

References 79 publications

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“…In this way, some extracts obtained from the food industry have proven their efficacy in this worm model. Extracts derived from honey displayed the potential to enhance mobility parameters in BR5706 worms [ 50 , 51 ]. For example, an avocado honey extract increased stretching efforts, swimming speed, and overall activity in BR5706-treated worms compared to untreated ones [ 51 ].…”

Section: Resultsmentioning

confidence: 99%

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Bioactive Properties of Tagetes erecta Edible Flowers: Polyphenol and Antioxidant Characterization and Therapeutic Activity against Ovarian Tumoral Cells and Caenorhabditis elegans Tauopathy

Rivas-García,

Crespo-Antolín,

Forbes-Hernández

et al. 2023

IJMS

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Tagetes erecta is an edible flower deeply rooted in traditional Mexican culture. It holds a central role in the most popular and iconic Mexican celebration, “the Day of the Dead”. Furthermore, it is currently receiving interest as a potential therapeutic agent, motivated mainly by its polyphenol content. The present study aims to evaluate the biological activity of an extract synthesized from the petals of the edible flower T. erecta. This extract showed significant antioxidant scores measured by the most common in vitro methodologies (FRAP, ABTS, and DPPH), with values of 1475.3 μM trolox/g extr, 1950.3 μM trolox/g extr, and 977.7 μM trolox/g extr, respectively. In addition, up to 36 individual polyphenols were identified by chromatography. Regarding the biomedical aspects of the petal extract, it exhibited antitumoral activity against ovarian carcinoma cells evaluated by the MTS assay, revealing a lower value of IC50 compared to other flower extracts. For example, the extract from T. erecta reported an IC50 value half as low as an extract from Rosa × hybrida and six times lower than another extract from Tulbaghia violacea. This antitumoral effect of T. erecta arises from the induction of the apoptotic process; thus, incubating ovarian carcinoma cells with the petal extract increased the rate of apoptotic cells measured by flow cytometry. Moreover, the extract also demonstrated efficacy as a therapeutic agent against tauopathy, a feature of Alzheimer’s disease (AD) in the Caenorhabditis elegans experimental model. Treating worms with the experimental extract prevented disfunction in several motility parameters such as wavelength and swimming speed. Furthermore, the T. erecta petal extract prevented the release of Reactive Oxygen Species (ROS), which are associated with the progression of AD. Thus, treatment with the extract resulted in an approximate 20% reduction in ROS production. These findings suggest that these petals could serve as a suitable source of polyphenols for biomedical applications.

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Section: Resultsmentioning

confidence: 99%

“…3.6.6. Acute Lethal Toxicity Assay Acute toxicity assay was carried out using the wild-type N2 synchronized L4 following the methodology proposed by Navarro-Hortal and collaborators [50]. The results obtained from this assay were employed to select the concentration for the assays.…”

Section: Mitochondrial Ros Content Determinationmentioning

confidence: 99%

Bioactive Properties of Tagetes erecta Edible Flowers: Polyphenol and Antioxidant Characterization and Therapeutic Activity against Ovarian Tumoral Cells and Caenorhabditis elegans Tauopathy

Rivas-García,

Crespo-Antolín,

Forbes-Hernández

et al. 2023

IJMS

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“…The total flavonoid content (TFC) was determined according to the aluminum chloride colorimetric method with some modifications [ 15 ]. Briefly, OL extracts and standards (catechin) were mixed with sodium nitrite for 6 min in a 96-well plate.…”

Section: Methodsmentioning

confidence: 99%

Exploring the Antioxidant, Neuroprotective, and Anti-Inflammatory Potential of Olive Leaf Extracts from Spain, Portugal, Greece, and Italy

Romero-Márquez,

Navarro-Hortal,

Forbes-Hernández

et al. 2023

Antioxidants

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The leaves of the olive tree (Olea europaea L.) are one of the major solid wastes from the olive industry. Globally, the European Union is the largest producer of olive by-products, with Spain, Italy, Greece, and Portugal accounting for almost the entire production. Many questions remain to be solved concerning olive leaves (OL), including those related to possible differences in composition and/or biological activities depending on their geographical origin. In the present work, OL from Spain, Italy, Greece, and Portugal have been characterized according to their phytochemical profile, antioxidant capacity, neuroprotective activity, and anti-inflammatory effects. The Spanish and Italian OL samples presented the highest antioxidant and neuroprotective activities, while the Greek OL showed the lowest. These results were strongly associated with the content of oleoside methyl ester and p-hydroxybenzoic acid for the Spanish and Italian samples, respectively, whereas the content of decarboxymethyl elenolic acid dialdehyde form (hydrated) was negatively associated with the mentioned biological activities of the Greek samples. No country-related effect was observed in the anti-inflammatory activity of OL. Comprehensively, this work could provide a useful tool for manufacturers and R&D departments in making environmentally friendly decisions on how OL can be used to generate nutraceutical products based on the composition and origin of this by-product.

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“…Certain by-products, including olive leaves, have also shown promise in combating AD [29]. Moreover, the potential therapeutic effects of honey in the context of AD have been explored (e.g., [30,31]). Additionally, over the several last years, an increasing number of studies in animal models have indicated the capability of different plant extracts used in traditional Chinese medicine to treat AD [32][33][34][35][36].…”

Section: Introductionmentioning

confidence: 99%

Another Use for a Proven Drug: Experimental Evidence for the Potential of Artemisinin and Its Derivatives to Treat Alzheimer’s Disease

Kiss,

Kins,

Gorgas

et al. 2024

IJMS

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Plant-derived multitarget compounds may represent a promising therapeutic strategy for multifactorial diseases, such as Alzheimer’s disease (AD). Artemisinin and its derivatives were indicated to beneficially modulate various aspects of AD pathology in different AD animal models through the regulation of a wide range of different cellular processes, such as energy homeostasis, apoptosis, proliferation and inflammatory pathways. In this review, we aimed to provide an up-to-date overview of the experimental evidence documenting the neuroprotective activities of artemi-sinins to underscore the potential of these already-approved drugs for treating AD also in humans and propose their consideration for carefully designed clinical trials. In particular, the benefits to the main pathological hallmarks and events in the pathological cascade throughout AD development in different animal models of AD are summarized. Moreover, dose- and context-dependent effects of artemisinins are noted.

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Amyloid β-but not Tau-induced neurotoxicity is suppressed by Manuka honeyviaHSP-16.2 and SKN-1/Nrf2 pathways in anin vivomodel of Alzheimer's disease

Abstract: Alzheimer's is a chronic degenerative disease of the central nervous system considered the first cause of dementia in the world. It is characterized by two etiopathological events related to oxidative...

Cited by 13 publications

References 79 publications

Bioactive Properties of Tagetes erecta Edible Flowers: Polyphenol and Antioxidant Characterization and Therapeutic Activity against Ovarian Tumoral Cells and Caenorhabditis elegans Tauopathy

Bioactive Properties of Tagetes erecta Edible Flowers: Polyphenol and Antioxidant Characterization and Therapeutic Activity against Ovarian Tumoral Cells and Caenorhabditis elegans Tauopathy

Exploring the Antioxidant, Neuroprotective, and Anti-Inflammatory Potential of Olive Leaf Extracts from Spain, Portugal, Greece, and Italy

Another Use for a Proven Drug: Experimental Evidence for the Potential of Artemisinin and Its Derivatives to Treat Alzheimer’s Disease

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