Amyrin esters induce cell death by apoptosis in HL-60 leukemia cells

Barros, Francisco; Bandeira, Paulo Nogueira; Lima, Daisy Jereissati Barbosa; Meira, Assuero Silva; Farias, Silvana Silveira de; Albuquerque, Maria Rose Jane R.; Santos, Hélcio Silva dos; Lemos, Telma L. G.; Morais, Manoel Odorico de; Costa-Lotufo, Letı́cia V.; Pessoa, Cláudia

doi:10.1016/j.bmc.2010.12.016

Cited by 46 publications

(27 citation statements)

References 44 publications

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“…β-Sitosterol-3-O-glucoside exhibited significant cytotoxicity against Caco-2 cell line compared to the control untreated cells. Statistical evaluation revealed low cytotoxicity of β-amyrin across all cell lines, consistent with published reports on the weak anticancer activity of some pentacyclic triterpenes stating that derivatives of these compounds may improve activity (Barros et al, 2011). …”

Section: Mtt Cell Viability Studiessupporting

confidence: 88%

Phytochemistry, Cytotoxicity and Apoptosis Studies of Β-Sitosterol-3-O-Glucoside and Β -Amyrin From Prunus Africana

Maiyo¹,

Moodley²,

Singh³

2016

Afr J Tradit Complement Altern Med

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Background: Prunus africana is used traditionally in many countries for the treatment of cancer and benign prostate hyperplasia. Materials and methods: In this study, compounds from the leaves and bark of this plant were isolated and tested for their cytotoxicity and apoptosis induction in two human cancer cell lines (hepatocellular carcinoma (HepG2) and colorectal carcinoma (Caco-2)) and a non-cancer cell line (embryonic kidney (HEK293)). GC-MS profiling of the extract was also conducted. Results: Three compounds (β-sitosterol, β-amyrin and β-sitosterol-3-O-glucoside) were isolated and the cytotoxic activity of β-amyrin and β-sitosterol-3-O-glucoside on the HepG2, Caco-2 and HEK293 was determined using the MTT cell viability assay. Both compounds had significant cytotoxic activity towards the Caco-2 cell line with IC50 values of 81 μg mL -1 and 54 μg mL -1 for β-amyrin and β-sitosterol-3-O-glucoside, respectively while low cytotoxicity was observed on HepG2 cell lines with IC50 values of 206 μg mL -1 and 251 μg mL -1 for β-amyrin and β-sitosterol-3-O-glucoside, respectively. Apoptosis induction in cells was studied using acridine orange/ethidium bromide dual staining. In both cases, the compounds tested demonstrated selective cytotoxicity towards cancer cells with high apoptosis indices in cells exposed to β-amyrin. Low IC50 values of 156 μg mL -1 and 937 μg mL -1 for β-amyrin and β-sitosterol-3-O-glucoside, respectively, were observed in the HEK293 cell line. Conclusion: This study reveals that the plant is rich biologically active compounds thereby validating its ethno-medicinal use.

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Section: Mtt Cell Viability Studiessupporting

confidence: 88%

Phytochemistry, Cytotoxicity and Apoptosis Studies of Β-Sitosterol-3-O-Glucoside and Β -Amyrin From Prunus Africana

Maiyo¹,

Moodley²,

Singh³

2016

Afr J Tradit Complement Altern Med

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“…However, ester derivatives of amyrin have been reported to induce apoptotic cell death in HL-60 leukemia cells. This study also reported the effect of these ester derivatives on cellular morphology, DNA fragmentation, cell cycle arrest, externalization of phosphatidylserine and activation of caspases 3/7 (Barros et al, 2011). It has also been reported that -and -amyrin isomers protects the liver against acetaminophen-induced injuries and ameliorates L-arginine-induced acute pancreatitis.…”

Section: Discussionmentioning

confidence: 77%

Design and synthesis of triazolyl-napthyl derivative of β-amyrin and its in vitro anti-cancer and apoptotic activities in human nasopharyngeal carcinoma (HK-1) cell line

Ge²,

Xie

et al. 2016

Bangladesh J Pharmacol

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The purpose of this research work was to design and synthesize triazolyl-napthyl derivative of β-amyrin (TNB) using click chemistry approach. Cytotoxic activity of TNB was evaluated against HK-1 human nasopharyngeal carcinoma cells using MTT cell viability assay. Fluorescence microscopy indicated cellular morphological changes induced by TNB. Flow cytometry was involved to demonstrate effects of this compound on cell cycle and apoptosis. The results revealed that TNB inhibited in vitro cancer cell growth in dose- and time-dependent manner. The IC50 values of TNB at 24 and 48 hours time intervals were found to be 47.2 and 32.5 µM respectively. TNB-treated cells exhibited significant dense staining fragmentation called apoptotic bodies, which inferred an early apoptotic event. DNA ladder was more apparent with the increasing TNB dose. However, no DNA fragments were observed in the control groups. TNB also induced sub-G1 cell cycle arrest and increased fraction of HK-1 apoptotic cells.

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“…Compounds 1 and 2 were identified from the MC fraction and elucidated as 22-dehydroclerosterol and β-amyrin (Fig. 1), respectively, by comparison with the spectral data described in the literature (Boar and Allen, 1973;Pech et al, 2002;Cho et al, 2005;Barros et al, 2011). The antibacterial activities of 22-dehydroclerosterol (1) and β-amyrin (2) against E. coli, S. aureus, and H. pylori are summarized in Table 2.…”

Section: Resultsmentioning

confidence: 87%

Antibacterial Activity of Triterpenoids from Clerodendron trichotomum

Choi¹,

Cho²,

Lee³

et al. 2012

Journal of Applied Biological Chemistry

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The aim of this research was to investigate the antibacterial activity of Clerodendron trichotomum. Antibacterial activities of the n-hexane, methylene chloride (MC), ethyl acetate, and n-butanol fractions from C. trichotomum were tested against Staphylococcus aureus, Escherichia coli, and Helicobacter pylori. The n-hexane and MC fractions showed antibacterial activity against H. pylori at a concentration of 1.7 mg/mL and showed inhibition zones of 10 and 11 mm in disc assay, respectively. Further testing of 22-dehydroclerosterol and β-amyrin (each 3.4 mg/mL) from the MC fraction of C. trichotomum revealed moderate antibacterial effects against E. coli, S. aureus, and H. pylori. In particular, β-amyrin showed clear zones of 12 and 13 mm against E. coli and H. pylori, respectively, suggesting its potential as an antibacterial agent. The active compounds from C. trichotomum might provide a promising therapeutic agent against infections by E. coli, S. aureus, and H. pylori.

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Amyrin esters induce cell death by apoptosis in HL-60 leukemia cells

Cited by 46 publications

References 44 publications

Phytochemistry, Cytotoxicity and Apoptosis Studies of Β-Sitosterol-3-O-Glucoside and Β -Amyrin From Prunus Africana

Phytochemistry, Cytotoxicity and Apoptosis Studies of Β-Sitosterol-3-O-Glucoside and Β -Amyrin From Prunus Africana

Design and synthesis of triazolyl-napthyl derivative of β-amyrin and its <i>in vitro</i> anti-cancer and apoptotic activities in human nasopharyngeal carcinoma (HK-1) cell line

Antibacterial Activity of Triterpenoids from Clerodendron trichotomum

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