An 8-year study on mycoplasma in rheumatoid arthritis.

Jansson, E; Mäkisara, P; Vainio, K; Vainio, Unto; Snellman, Olle; Tuuri, Sirkka

doi:10.1136/ard.30.5.506

Cited by 33 publications

(10 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…of synovial fluids taken from patients with rheumatoid arthritis has been reported by Williams (1967a, b;1970). Although other studies have suggested an association between this disease and other species of mycoplasma (Bartholomew, 1965;Jansson and Wager, 1967;Jansson, Makisara, Vainio, Vainio, Snellman, and Tuuri, 1971;Fraser, Shirodaria, Haire, and Middleton, 1971), the possibility that M. fermentans was associated with rheumatoid arthritis was supported by the finding that there was inhibition of peripheral leucocyte migration in the presence ofextracts of M.fermentans, indicating the existence of a specific immunological response (Williams, Brostoff, and Roitt, 1970). In view of the importance of such an association between M. fermentans and rheumatoid arthritis, we have attempted to isolate mycoplasmas from synovial -fluids by techniques including those described by Williams. Methods Source ofsynovialfluids Synovial fluids were obtained from knee effusions by needle aspiration under aseptic conditions from 43 patients with rheumatoid arthritis and five non-rheumatoid patients ( Table 1).…”

mentioning

confidence: 96%

Search for mycoplasma in synovial fluids from patients with rheumatoid arthritis.

Windsor¹,

Nicholls²,

Maini³

et al. 1974

Annals of the Rheumatic Diseases

View full text Add to dashboard Cite

mentioning

confidence: 96%

Search for mycoplasma in synovial fluids from patients with rheumatoid arthritis.

Windsor¹,

Nicholls²,

Maini³

et al. 1974

Annals of the Rheumatic Diseases

View full text Add to dashboard Cite

“…Perhaps the most striking observation was that all of these isolates proved to be serologically identical, as par ticularly shown by the disc-growth inhibition test, and serologically closely related to, or rather identical with, the rat mycoplasma M. arthritidis, which they resemble also in metabolizing arginine. The serological classification of Jansson's strains as M. arthritidis has been confirmed, in the case of the two first isolates, by Lemcke and by Hayflick [88], The suggestion by Jansson et al [85,90] that their strains share antigens with a T-mycoplasma strain isolated from NGU lacks documentation and is sur prising in view of the fact that the T-mycoplasmas have been found by all other workers to be serologically very distinct, as compared to the 'classical' mycoplasmas. In their growth requirements the rheumatoid arthritis strains are apparently much more fastidious than other strains of M. arthritidis, growth of very tiny colonies of atypical appearance only being obtained on a medium of relatively complex composition [83].…”

Section: Manmentioning

confidence: 54%

Present Status of the Medical Importance of Mycoplasmas

Ea¹

1974

Pathobiology

View full text Add to dashboard Cite

According to their tissue affinities and pathogenicity, the mycoplasmas may be placed into three maingroups: (1) those associated with respiratory tract infections; (2) those involved in infections of the urogenital tract; and (3) mycoplasmas showing a particular affinity for joint tissues.On the basis of this grouping, presently available data relating several Mycoplasma and Acholeplasma species with diseases of man and animals are critically reviewed. The importance of comparative studies in the field of mycoplasmal pathology is emphasized.

show abstract

“…Cultures that yielded BLE were recorded separately from the month after first isolation. Statistical analysis was by methods recommended by Langley (1979).…”

Section: Discussionmentioning

confidence: 99%

“…Diphtheroids (Stewart, Alexander and Duthie, 1969;Duthie, Stewart and McBride, 1976) and Mycoplasma (Bartholomew, 1965;Jansson et al, 1971) have been isolated more often from synovial membranes and fluids of than one culture was made from a single sample, they were labelled sequentially at the time of inoculation.…”

Section: Bacillus Licheniformis Var Endoparasiticusmentioning

confidence: 99%

Isolation of Bacillus licheniformis var. endoparasiticus from the blood of rheumatoid arthritis patients and normal subjects

Bartlett¹,

Bisset²

1981

Journal of Medical Microbiology

View full text Add to dashboard Cite

Sixty-five samples of blood from patients with rheumatoid arthritis and 94 from control subjects were examined by multiple culture and monthly subculture for periods of up to 33 months to detect the reversion stages of Bacillus licheniformis var. endoparasiticus (BLE), which exists as L-forms in blood. Isolates of BLE were obtained more often from the blood of control subjects than from rheumatoid patients during the first 6 months of incubation, when there was clustering of positive cultures within samples. Thereafter, the isolation rate was similar for the two groups and positive cultures were distributed randomly between samples. Isolation of diphtheroid intermediate reversion stages (phases A and B) occurred mainly during the first year of incubation, but isolations of the fully reverted sporing bacillus (phase C) increased in frequency with incubation time, particularly from cultures with a high rate of desiccation during prolonged incubation. The proportion of different phases of BLE amongst the isolates and the distribution of the phases with incubation time were similar for the rheumatoid-arthritis patients and the normal subjects. 98 ROBERTA BARTLETT AND K. A. BISSET rheumatoid arthritis patients than from those of patients with non-rheumatoid joint conditions. Bartholomew and Nelson (1972) have reported a higher isolation rate of the anaerobic or microaerophilic "Corynebacterium acnes" from the blood of rheumatoid patients than from non-rheumatoids. Previous work in this laboratory (Pease, 1974; Bisset and Bartlett, 1978) has produced evidence that the organisms resembling Mycoplasma and those described as diphtheroid bacteria, isolated from the blood of rheumatoid patients and from normal persons (Benedek, 1955; Pease, 1969,1970; Duthie et al., 1976; Markham and Myers, 1976; Tedeschi et al., 1978), are L-forms and reversion stages of B. licheniformis var. endoparasiticus, hereafter referred to as BLE. MATERIALS AND METHODS Blood samples Collection of samples. A total of 164 blood samples were examined, 65 were from rheuma-toid arthritis patients, five from patients with other arthritic conditions, and 94 from controls. Fifty-five samples of blood from healthy donors were taken by courtesy of the West Midland Regional Blood Transfusion Service; 1 O-ml samples were collected in 1-ml sterile acid-citrate-dextrose anticoagulant at the end of the donation from the tube that remained connected to the donor's vein, by the method and with the precautions previously described (Bisset and Bartlett, 1978). Twenty-one samples of blood from laboratory staff were taken by Dr M. Synnott of the Department of Medical Microbiology, University of Birmingham. The donor's skin was disinfected with 70% isopropyl alcohol (Sterets Injection Swab, Prebbles Medical Ltd, Mersey-side); 5 ml of blood was taken into disposable hypodermic syringes and transferred to sterile screw-capped bottles containing 0.25 ml of 0.4% (w/v) aqueous heparin. The same technique was used for the collection of two samples of blood from laboratory st...

show abstract

An 8-year study on mycoplasma in rheumatoid arthritis.

Cited by 33 publications

References 3 publications

Search for mycoplasma in synovial fluids from patients with rheumatoid arthritis.

Search for mycoplasma in synovial fluids from patients with rheumatoid arthritis.

Present Status of the Medical Importance of Mycoplasmas

Isolation of Bacillus licheniformis var. endoparasiticus from the blood of rheumatoid arthritis patients and normal subjects

Contact Info

Product

Resources

About