An ABA-responsive element in the AtSUC1 promoter is involved in the regulation of AtSUC1 expression

Hoth, Stefan; Niedermeier, Matthias; Feuerstein, Andrea; Hornig, Julia; Sauer, Norbert

doi:10.1007/s00425-010-1228-4

Cited by 41 publications

(39 citation statements)

References 70 publications

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“…MdSUT1 was induced by gibberellic acid and abscisic acid but not by auxin (Peng et al 2011). Abscisic acid responsive elements were also found to be important for the regulation of expression of AtSUC1 gene (Hoth et al 2011). In silico analysis indicated the presence of ethylene responsive element in sucrose transporters (Ibraheem et al 2010).…”

Section: Expression Of Ntsut1 Is Auxin-dependentmentioning

confidence: 97%

Sucrose transporter NtSUT1 confers aluminum tolerance on cultured cells of tobacco (Nicotiana tabacumL.)

Sameeullah

Sasaki

Yamamoto

2013

Soil Science and Plant Nutrition

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The role of plasma membrane-localized sucrose transporter (NtSUT1) was investigated using cultured tobacco cell (Nicotiana tabacum L.) line BY-2. The wild type (WT) cells were first transformed with the NtSUT1 gene or its fragments cloned from tobacco cell line SL to form the over-expression (OX) and suppression (RNAi) cell lines, respectively. Using OX and RNAi transgenics, the role of NtSUT1 in growth capacity of actively growing cells and in aluminum (Al)-treated cells was examined. During the logarithmic phase of growth in nutrient medium containing 2,4-dichlorophenoxyacetic acid (2,4-D), both the rate of sucrose uptake measured with radio-tracer and the content of soluble sugars were higher in OX and lower in RNAi cell lines compared to WT. Overall, the content of soluble sugars negatively correlated with the time necessary for doubling mass (fresh weight). When cells were treated without (control) or with Al in a simple medium containing calcium, sucrose and 2-(N-morpholino)ethanesulfonic acid (MES; pH 5.0) for up to 18 h, the expression of NtSUT1 under its native promoter, or under the control of strong constitutive cauliflower mosaic virus (CaMV) 35S promoter, was strongly dependent on the presence of 2,4-D. Thereafter, the cells were preferentially treated in the presence of 2,4-D. During 6 h after a start of the control treatment, sucrose uptake rates were, compared to WT, slightly higher and lower in OX and RNAi lines respectively. The addition of Al reduced the sucrose uptake rates of OX and WT to the level of RNAi line, indicating that Al inhibits sucrose uptake via NtSUT1. During the post-Al culture of control and Al-treated cells in a nutrient medium, sucrose uptake rates were much higher in OX compared to WT and RNAi lines, which closely and positively correlated with the growth capacity of the cells. Judging from the growth capacity of Al-treated cells relative to that of control cells, OX cells were more tolerant to Al than WT and RNAi. In summary, we conclude that over-expression of NtSUT1 confers higher growth capacity in actively growing cells as well as in Al-treated cells.

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Section: Expression Of Ntsut1 Is Auxin-dependentmentioning

confidence: 97%

Sucrose transporter NtSUT1 confers aluminum tolerance on cultured cells of tobacco (Nicotiana tabacumL.)

Sameeullah

Sasaki

Yamamoto

2013

Soil Science and Plant Nutrition

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“…Another SUT whose expression was found to consistently respond to abiotic stress is AtSUC1. It has been previously implicated in ABA signaling (Hoth et al, 2010) and sugar signaling (Sivitz et al, 2008) in roots and pollen. In contrast to those tissues, AtSUC1 expression in mature leaves is very low, and its domain of expression is most likely limited to developing trichomes (Sivitz et al, 2007).…”

Section: Sut Regulation Is Part Of the Abiotic Stress Responsementioning

confidence: 99%

Regulation of Sucrose Transporters and Phloem Loading in Response to Environmental Cues

et al. 2017

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Suc transporters (SUTs) play a key role in the allocation and partitioning of photosynthetically fixed carbon in plants. While a function could be assigned to many members of the SUT family, almost no information is available on their regulation. Here, the transcriptional regulation of SUTs in response to various environmental stimuli in the leaves of five dicots (Arabidopsis Extensive data on expression of SUTs in relation to changes of environmental conditions were obtained through a global analysis of 168 transcriptomics data sets. Results were validated by quantitative PCR measurements and extended by the measurement of photosynthesis rate and phloem sugar content to draw insight on the correlation of SUT expression and sugar export from leaves. For the apoplasmic phloem loaders, a clear difference in transcriptional regulation in response to different environmental stimuli was observed. The consistent patterns of SUT expression under abiotic stress indicates which types of SUTs are involved in the regulation of leaf sugar status and in stress signaling. Furthermore, it is shown that down-regulation of phloem loading is likely to be caused by transcriptional regulation of SUTs, while up-regulation depends on post-transcriptional regulation. In poplar, expression of PtaSUT4 was found to consistently respond to environmental stimuli, suggesting a significant role in the regulation of sugar export from leaves in this passive symplasmic phloem loader.

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“…However, no direct connection has yet been identified between Suc, light, hormone signaling, and SUC regulation in Arabidopsis. Plant hormones such as auxin and abscisic acid (Jeong et al, 2004;Hoth et al, 2010), gibberellins (Weiss et al, 1995), cytokinin (Deikman and Hammer, 1995), and ethylene (Morgan and Drew, 1997) differentially regulate anthocyanin biosynthesis in whole plants as well as in cell suspensions (Ozeki and Komamine, 1986). Ethylene markedly suppresses anthocyanin accumulation (Craker and Wetherbee, 1973;Kang and Burg, 1973), while the Co 2+ -mediated inhibition of ethylene biosynthesis and the prevention of ethylene activity by silver increases the anthocyanin content of corn seedlings (Rengel and Kordan, 1987).…”

mentioning

confidence: 99%

Ethylene Suppression of Sugar-Induced Anthocyanin Pigmentation in Arabidopsis

et al. 2010

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Anthocyanin accumulation is regulated negatively by ethylene signaling and positively by sugar and light signaling. However, the antagonistic interactions underlying these signalings remain to be elucidated fully. We show that ethylene inhibits anthocyanin accumulation induced by sucrose (Suc) and light by suppressing the expression of transcription factors that positively regulate anthocyanin biosynthesis, including GLABRA3, TRANSPARENT TESTA8, and PRODUCTION OF AN-THOCYANIN PIGMENT1, while stimulating the concomitant expression of the negative R3-MYB regulator MYBL2. Genetic analyses show that the ethylene-mediated suppression of anthocyanin accumulation is dependent upon ethylene signaling components responsible for the triple response. Furthermore, these positive and negative signaling pathways appear to be under photosynthetic control. Suc and light induction of anthocyanin accumulation was almost fully inhibited in wild-type Arabidopsis (Arabidopsis thaliana) ecotype Columbia and ethylene (ethylene response1 [etr1-1]) and light (long hypocotyl1 [hy1], cryptochrome1/2, and hy5) signaling mutants treated with the photosynthetic electron transport inhibitor 3-(3,4-dichlorophenyl)-1,1-dimethylurea. The transcript level of the sugar transporter gene SUC1 was enhanced in ecotype Columbia treated with the ethylene-binding inhibitor silver and in etr1-1, ethylene insensitive2 (ein2-1), and ein3 ein3-like1 mutants. In contrast, 3-(3,4-dichlorophenyl)-1,1-dimethylurea treatment reduced SUC1 expression, which indicates strongly that SUC1 represents an integrator for signals provided by sugar, light, and ethylene. SUC1 mutations lowered accumulations of anthocyanin pigment, soluble sugar content, and ethylene production in response to Suc and light signals. These data demonstrate that the suppression of SUC1 expression by ethylene inhibits Suc-induced anthocyanin accumulation in the presence of light and, hence, fine-tunes anthocyanin homeostasis.Anthocyanins play key roles in many plant physiological processes; for instance, they form photoprotective screens in vegetative tissue, act as visual attractors to aid pollination and seed dispersal, and function as antimicrobial agents and feeding deterrents in the defense response (Winkel-Shirley, 2001;Steyn et al., 2002). The anthocyanin biosynthetic pathway is well described in plants. In Arabidopsis (Arabidopsis thaliana) and other plants, including Antirrhinum majus (snapdragon) and Petunia hybrida (petunia), early biosynthesis genes (EBGs) such as chalcone synthase (CHS), chalcone isomerase (CHI), flavanone 3-hydroxylase (F3H), and flavonoid 3#-hydroxylase (F3#H), which are common to different flavonoid subpathways, are induced prior to late biosynthesis genes (LBGs) such as dihydroflavonol 4-reductase (DFR), leucoanthocyanidin oxygenase (LDOX), anthocyanidin reductase (ANR), and UDP-glucose:flavonoid 3-O-glucosyltransferase (UF3GT; Pelletier et al., 1997). EBGs and LBGs are transcriptionally activated by R2R3-MYBs, such as PRODUCTION OF ANTHOCYANIN PIG-MENT1 (PAP1) a...

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An ABA-responsive element in the AtSUC1 promoter is involved in the regulation of AtSUC1 expression

Cited by 41 publications

References 70 publications

Sucrose transporter NtSUT1 confers aluminum tolerance on cultured cells of tobacco (Nicotiana tabacumL.)

Sucrose transporter NtSUT1 confers aluminum tolerance on cultured cells of tobacco (Nicotiana tabacumL.)

Regulation of Sucrose Transporters and Phloem Loading in Response to Environmental Cues

Ethylene Suppression of Sugar-Induced Anthocyanin Pigmentation in Arabidopsis

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