An abnormal lipid-like material and carbohydrate in the sera of patients with multiple myeloma

Sachs, Bernard A.; Cady, P.; Ross, George

doi:10.1016/0002-9343(54)90025-x

Cited by 49 publications

(5 citation statements)

References 15 publications

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“…Gamma globulin separated from normal serum using filter paper electrophoresis has been variously reported to be periodic acid-Schiff (PAS) negative (11,12) and positive (13,14). Myeloma proteins have been stained with PAS after paper electrophoresis and were found to exhibit an intense PAS reaction (11,15,16). This finding has been interpreted to indicate that the myeloma proteins represent abnormal glycoproteins.…”

mentioning

confidence: 99%

The CARBOHYDRATE OF Γ-Globulin AND MYELOMA PROTEINS

Müller‐Eberhard

Kunkel

1956

The Journal of Experimental Medicine

146

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Fractions of serum consisting primarily of 7-globulin as determined by electrophoresis have been shown to contain protein-bound carbohydrate (1-3). However, recent evidence concerning the electrophoretic inhomogeneity of ~/-globulin (4-6) as well as the finding of secondary components in the ultracentrifuge (7) which might be high in carbohydrate has caused uncertainty regarding the existence of carbohydrate as a distinct constituent of the various species of proteins making up this fraction of serum.The purpose of the present study was to investigate quantitatively the carbohydrate content of various components of serum "r-globulin as well as the pathological myeloma proteins which are immunologicaUy related to these normal proteins (8-10). Five preparations of ~/-globulin and twelve myeloma proteins were isolated electrophoretically and analyzed for hexose, fucose, hexosamine, and sialic acid. A method of zone electrophoresis was developed employing a polyvinyl chloride supporting medium which was particularly suited for carbohydrate analyses of separated fractions. It was found that various *t-globulin preparations from normal and pathological sera which were homogeneous in the ultracentrifuge contain a similar amount of carbohydrate. This was also true of certain of the myeloma proteins of the ~/-globulin type. The fl-myeloma proteins showed a higher carbohydrate content.Gamma globulin separated from normal serum using filter paper electrophoresis has been variously reported to be periodic acid-Schiff (PAS) negative (11, 12) and positive (13,14). Myeloma proteins have been stained with PAS after paper electrophoresis and were found to exhibit an intense PAS reaction (11,15,16). This finding has been interpreted to indicate that the myeloma proteins represent abnormal glycoproteins. These studies, however, were not quantitative and the protein-carbohydrate ratio could not be determined for comparison with normal ~/-globulin. Various myeloma proteins have been found to differ markedly in electrophoretic mobility (17) and in their immunological specificity (8,18,19). The possible role of the carbohydrate constituents of these proteins in accounting for their differences has not been ascertained, although some evidence on this point has recently been reported by Smith and associates (10) and from this laboratory (20). Determination

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confidence: 99%

The CARBOHYDRATE OF Γ-Globulin AND MYELOMA PROTEINS

Müller‐Eberhard

Kunkel

1956

The Journal of Experimental Medicine

146

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“…Sachs et al (1954) found that the total serum lipid was raised in 3 out of 11 patients (highest value 1.4'g. /100 ml.)…”

Section: Discussionmentioning

confidence: 89%

“…He found that the abnormal /2globulin contained about 6% of polysaccharides, half of which was hexose. The presence of carbohydrate in abnormal myeloma proteins has been noted previously (Sachs et al, 1954;Osserman and Lawlor, 1954). Dr. K. W. Walton measured the cholesterol content of the a and /8 zones (Nury and Smith, 1957) and found that only 30 mg. (normal 30-70 mg./100 ml.)…”

Section: Serum Proteinsmentioning

confidence: 86%

Myelomatosis with Lipaemia and Xanthomata

Lennard‐Jones¹

1960

BMJ

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“…Hexoses in serum albumin fraction: elevated in normal pregnancy (29), more so in carcinoma (17) and rheumatic fever (9). In multiple myeloma, the pathological protein contains maximum amount of polysaccharides (30). In pneumonia, polysaccharides are high in all electrophoretic fractions (31).…”

Section: Methodsmentioning

confidence: 99%