An acetylcholinesterase (AChE) biosensor with enhanced solvent resistance based on chitosan for the detection of pesticides

Warner, John C.; Andreescu, Silvana

doi:10.1016/j.talanta.2015.08.030

Cited by 35 publications

(22 citation statements)

References 29 publications

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“…Fortunately, the development of new, selective and sensitive sensors allows for increasingly easy and cheap ways to monitor and detect toxic and hazardous substances in the environment [11][12][13][14], possibly also permitting individual citizens in the near future, to screen for different contaminants. Several biosensors for OP detection based on enzymatic or biological (cells and microorganisms) bioreceptors have been previously proposed [15][16][17]. The most known are biosensors based on the inhibition of the activity of acetylcholinesterase [17,18], which is the target of Ops' mechanism of action.…”

Section: Introductionmentioning

confidence: 99%

Highly Sensitive Detection of Chemically Modified Thio-Organophosphates by an Enzymatic Biosensing Device: An Automated Robotic Approach

Cetrangolo

Ruško

Gori

et al. 2020

Sensors

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Pesticides represent some of the most common man-made chemicals in the world. Despite their unquestionable utility in the agricultural field and in the prevention of pest infestation in public areas of cities, pesticides and their biotransformation products are toxic to the environment and hazardous to human health. Esterase-based biosensors represent a viable alternative to the expensive and time-consuming systems currently used for their detection. In this work, we used the esterase-2 from Alicyclobacillus acidocaldarius as bioreceptor for a biosensing device based on an automated robotic approach. Coupling the robotic system with a fluorescence inhibition assay, in only 30 s of enzymatic assay, we accomplished the detection limit of 10 pmol for 11 chemically oxidized thio-organophosphates in solution. In addition, we observed differences in the shape of the inhibition curves determined measuring the decrease of esterase-2 residual activity over time. These differences could be used for the characterization and identification of thio-organophosphate pesticides, leading to a pseudo fingerprinting for each of these compounds. This research represents a starting point to develop technologies for automated screening of toxic compounds in samples from industrial sectors, such as the food industry, and for environmental monitoring.

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Section: Introductionmentioning

confidence: 99%

Highly Sensitive Detection of Chemically Modified Thio-Organophosphates by an Enzymatic Biosensing Device: An Automated Robotic Approach

Cetrangolo

Ruško

Gori

et al. 2020

Sensors

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“…Most of the paraoxon and dichlorovos are hydrophobic or only slightly soluble in aqueous solution, but they easily dissolve in organic solvents. AChE from the electric eel had been studied in different organic solvents and electrodes for paroxon determination . In this study, we analyzed paraoxon and dichlorovos in a water/ methanol solution.…”

Section: Resultsmentioning

confidence: 99%

“…AChE from the electric eel had been studied in different organic solvents and electrodes for paroxon determination. 26 In this study, we analyzed paraoxon and dichlorovos in a water/ methanol solution. The effect of methanol concentration is shown in Figure 4.…”

Section: Effect Of Stopping Time and Methanolmentioning

confidence: 99%

Analysis of Organophosphorous Pesticides Based on Housefly Acetylcholinesterase Using Sequential Injection Analysis

Shu

Chung

2017

J Chinese Chemical Soc

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Organophosphorous pesticides were analyzed based on the inhibition of acetylcholinesterase (AChE) from housefly and the electric eel using a sequential injection analysis system. The linear ranges for paraoxon of 1–70 ppb) and dichlorovos of 20–70 ppb were obtained, and the detection limits of paraoxon and dichlorovos were 1 and 5 ppb, respectively. AChE from housefly shows 10 times higher sensitivity than that from eel.

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“…Incorporation of electro-catalyst in the biosensor can promote bioreactions among the biomolecules sensitivity (Khan et al 2008;Safavi and Farjami 2011). In addition, the chitosan films (Warner and Andreescu 2016) and nanocomposites of chitosan with PB (Zhao et al 2015) were also used to improve pesticide biosensors. Thus, the use of marine polysaccharides as fundamental materials for various biosensors have demonstrated a strong potential for the improvement of biosensor functions.…”

Section: Immobilization Of Biomolecules For Improvement Of Biosensor mentioning

confidence: 99%

“…Khan et al (2008),Wang et al (2009), Wu et al (2009,Safavi and Farjami (2011), Zeng et al (2011),Kavitha et al (2013),Su et al (2013), Ş enel(2015),Zhao et al (2015) andWarner and Andreescu (2016) Inhibition of infection on implant sitesBumgardner et al (2003),Alex et al (2008),Swanson et al (2011), Wiarachai et al (2012,Honglue et al (2013) andPishbin et al (2014) …”

mentioning

confidence: 99%

Marine polysaccharides: therapeutic efficacy and biomedical applications

et al. 2017

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The ocean contains numerous marine organisms, including algae, animals, and plants, from which diverse marine polysaccharides with useful physicochemical and biological properties can be extracted. In particular, fucoidan, carrageenan, alginate, and chitosan have been extensively investigated in pharmaceutical and biomedical fields owing to their desirable characteristics, such as biocompatibility, biodegradability, and bioactivity. Various therapeutic efficacies of marine polysaccharides have been elucidated, including the inhibition of cancer, inflammation, and viral infection. The therapeutic activities of these polysaccharides have been demonstrated in various settings, from in vitro laboratory-scale experiments to clinical trials. In addition, marine polysaccharides have been exploited for tissue engineering, the immobilization of biomolecules, and stent coating. Their ability to detect and respond to external stimuli, such as pH, temperature, and electric fields, has enabled their use in the design of novel drug delivery systems. Thus, along with the promising characteristics of marine polysaccharides, this review will comprehensively detail their various therapeutic, biomedical, and miscellaneous applications.

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An acetylcholinesterase (AChE) biosensor with enhanced solvent resistance based on chitosan for the detection of pesticides

Cited by 35 publications

References 29 publications

Highly Sensitive Detection of Chemically Modified Thio-Organophosphates by an Enzymatic Biosensing Device: An Automated Robotic Approach

Highly Sensitive Detection of Chemically Modified Thio-Organophosphates by an Enzymatic Biosensing Device: An Automated Robotic Approach

Analysis of Organophosphorous Pesticides Based on Housefly Acetylcholinesterase Using Sequential Injection Analysis

Marine polysaccharides: therapeutic efficacy and biomedical applications

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