An acetylcholinesterase biosensor based on doping Au nanorod@SiO2 nanoparticles into TiO2-chitosan hydrogel for detection of organophosphate pesticides

Cui, Hui‐Fang; Zhang, Tingting; Lv, Qi‐Yan; Song, Xiaojie; Zhai, Xiao-Jing; Wang, Gai-Gai

doi:10.1016/j.bios.2019.111452

Cited by 92 publications

(24 citation statements)

References 40 publications

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“…Km value assessed from this representation is 0.43 mM. This value is higher than the value 0.258 mM obtained with Mahmoudi team [40] but smaller than 1.89 mM obtained by the Cui team [41].…”

Section: Enzyme Activity Assessment Of Immobilized Achecontrasting

confidence: 58%

Acetylcholinesterase Modified Porous Silicon for Electrochemical Measurement of Total Active Immobilized Enzyme Amount and Effective Malathion Detection

Khaldi¹,

Sam²,

Gabouze³

2020

I2M

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An amperometric method to measure the total amount of active and inhibited immobilized Acetylcholinesterase enzyme (AChE) and the quantification of AChE inhibitors was realized. In this approach porous silicon (PSi) surface was used as a matrix for AChE immobilization and Boron doped polycrystalline diamant (BDPD) was used as working electrode for the electrochemical measurements. The covalent immobilization of AChE from electric eel was achieved on amine functionalized PSi surface previously decorated with Au particles. This surface is suitable for a stable attachment of AChE enzyme. The amperometric detection of AChE activity at Boron doped polycrystalline diamond electrode is based on the oxidation of thiocholine, the enzymatic reaction product of immobilized AChE in the presence of the substrate acetylthiocholine chlorid (ATCl) and this without the need to further modify the BDPD surface or the use of other reagents. The concentration of immobilized active AChE enzymes was estimated to Γ≈ 1.8 10 12 AChE cm -2 by means of a calibration curve. Michaelis constant was assessed with a Km of 4.3 10 -4 M. Finally, the electrochemical quantification and detection of Malathion shows a good linear calibration curve where the concentration of Malathion range is from 2 to 6 nM within 6 minutes after inhibitor addition.

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Section: Enzyme Activity Assessment Of Immobilized Achecontrasting

confidence: 58%

Acetylcholinesterase Modified Porous Silicon for Electrochemical Measurement of Total Active Immobilized Enzyme Amount and Effective Malathion Detection

Khaldi¹,

Sam²,

Gabouze³

2020

I2M

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“…In the presence of OPs, the amount of TCl would be reduced due to the inhibition of AChE. According to this mechanism, the OPs residue of the analyte can be easily detected by the electrochemical biosensor [10][11][12][13][14].…”

Section: Introductionmentioning

confidence: 99%

Acetylcholinesterase electrochemical biosensors with graphene-transition metal carbides nanocomposites modified for detection of organophosphate pesticides

Wang

et al. 2020

PLoS ONE

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An acetylcholinesterase biosensor modified with graphene and transition metal carbides was prepared to detect organophosphorus pesticides. Cyclic voltammetry, differential pulse voltammetry, and electrochemical impedance spectroscopy were used to characterize the electrochemical catalysis of the biosensor: acetylcholinesterase/chitosan-transition metal carbides/graphene/glassy carbon electrode. With the joint modification of graphene and transition metal carbides, the biosensor has a good performance in detecting dichlorvos with a linear relationship from 11.31 μM to 22.6 nM and the limit of detection was 14.45 nM. Under the premise of parameter optimization, the biosensor showed a good catalytic performance for acetylcholine. Compared to the biosensors without modification, it expressed a better catalytic performance due to the excellent electrical properties, biocompatibility and high specific surface area of graphene, transition metal carbides. Finally, the biosensor exhibits good stability, which can be stored at room temperature for one month without significant performance degradation, and has practical potential for sample testing.

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“…Au nanorods have excellent optical properties [84] , [85] , [86] . In this way, Cui and colleagues developed an electrochemical acetylcholine esterase biosensor using the high scattering cross-section of Au nanorods and their capacity to form core–shell nanostructures with mesoporous silica [87] . The LOD for dichlorvos and dithiophos were 1.2 and 0.36 µg/L, respectively.…”

Section: Classification and Application Of Enzyme Inhibition Methods ...mentioning

confidence: 99%

Enzyme inhibition methods based on Au nanomaterials for rapid detection of organophosphorus pesticides in agricultural and environmental samples: A review

Zhai

Chen

Liu

et al. 2022

Journal of Advanced Research

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An acetylcholinesterase biosensor based on doping Au nanorod@SiO2 nanoparticles into TiO2-chitosan hydrogel for detection of organophosphate pesticides

Cited by 92 publications

References 40 publications

Acetylcholinesterase Modified Porous Silicon for Electrochemical Measurement of Total Active Immobilized Enzyme Amount and Effective Malathion Detection

Acetylcholinesterase Modified Porous Silicon for Electrochemical Measurement of Total Active Immobilized Enzyme Amount and Effective Malathion Detection

Acetylcholinesterase electrochemical biosensors with graphene-transition metal carbides nanocomposites modified for detection of organophosphate pesticides

Enzyme inhibition methods based on Au nanomaterials for rapid detection of organophosphorus pesticides in agricultural and environmental samples: A review

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