An active site mutation in 6-hydroxy-l-Nicotine oxidase from Arthrobacter nicotinovorans changes the substrate specificity in favor of (S)-nicotine

Deay, Dwight O.; Colvert, Kim K.; Gao, Fei; Seibold, Steve A.; Goyal, Priyanka; Aillon, Daniel V.; Petillo, Peter A.; Richter, Mark L.

doi:10.1016/j.abb.2020.108520

Cited by 4 publications

(2 citation statements)

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“…Previous studies have demonstrated three main pathways of bacterial nicotine degradation. In the pyridine degradation pathway represented by the Gram-positive bacteria Arthrobacter , the main degradation enzymes include nicotine dehydrogenase, 6-hydroxynicotine oxidase, 6-hydroxypseudooxynicotine dehydrogenase, 2,6-dihydroxypseudooxynicotine hydrolase, 2,6-dihydroxypyridine 3-monooxygenase, and nicotine blue oxidoreductase (Sachelaru et al 2005 ; Mihasan et al 2007 ; Schenk et al 1998 ; Deay et al 2020 ). In the pyrrolidine pathway represented by the Gram-negative bacteria Pseudomonas , the most important enzymes are nicotine oxidoreductase and 3-succinoylpyridine monooxygenase (Hu et al 2019 ; Xia et al 2018 ).…”

Section: Introductionmentioning

confidence: 99%

Cultivation and application of nicotine-degrading bacteria and environmental functioning in tobacco planting soil

Wang

Luo

Chu

et al. 2023

Bioresour. Bioprocess.

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Nicotine, a toxic and addictive alkaloid from tobacco, is an environmental pollutant. However, nicotine-degrading bacteria (NDB) and their function in tobacco planting soil are not fully understood. First, 52 NDB strains belonging to seven genera were isolated from tobacco soil. The most dominant genera were Flavobacterium (36.5%), Pseudomonas (30.8%), and Arthrobacter (15.4%), and Chitinophaga and Flavobacterium have not been previously reported. Then, two efficient NDB strains, Arthrobacter nitrophenolicus ND6 and Stenotrophomonas geniculata ND16, were screened and inoculated in the compost fertilizer from tobacco waste. The nicotine concentrations were reduced from 1.5 mg/g (DW) to below the safety threshold of 0.5 mg/g. Furthermore, strain ND6 followed the pyridine pathway of nicotine degradation, but the degrading pathway in strain ND16 could not be determined according to genomic analysis and color change. Finally, the abundance of nicotine-degrading genes in tobacco rhizosphere soil was investigated via metagenomic analysis. Five key genes, ndhA, nctB, kdhL, nboR, and dhponh, represent the whole process of nicotine degradation, and their abundance positively correlated with soil nicotine concentrations (p < 0.05). In conclusion, various NDB including unknown species live in tobacco soil and degrade nicotine efficiently. Some key nicotine-degrading genes could be used in monitoring nicotine degradation in the environment. The fermentation of compost from tobacco waste is a promising application of efficient NDB. Graphical Abstract

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Section: Introductionmentioning

confidence: 99%

Cultivation and application of nicotine-degrading bacteria and environmental functioning in tobacco planting soil

Wang

Luo

Chu

et al. 2023

Bioresour. Bioprocess.

View full text Add to dashboard Cite

show abstract

“…Previous studies have demonstrated three main pathways of bacterial nicotine degradation. In the pyridine degradation pathway represented by the Gram-positive bacteria Arthrobacter, the main degradation enzymes include nicotine dehydrogenase, 6-hydroxynicotine oxidase, 6hydroxypseudooxynicotine dehydrogenase, 2,6-dihydroxypseudooxynicotine hydrolase, 2,6dihydroxypyridine 3-monooxygenase and nicotine blue oxidoreductase (Sachelaru et al, 2005;Mihasan et al, 2007;Schenk et al, 1998;Deay et al,2020). In the pyrrolidine pathway represented by the Gramnegative bacteria Pseudomonas, the most important enzymes are nicotine oxidoreductase, and 3succinoylpyridine monooxygenase (Hu et al, 2019;Xia et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Cultivation and application of nicotine-degrading bacteria and environmental functioning in tobacco planting soil

Wang

Luo

Chu

et al. 2022

Preprint

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Nicotine, a toxic and addictive alkaloid from tobacco, is an environmental pollutant. However, nicotine-degrading bacteria (NDB) and their function in tobacco planting soil are not fully understood. First, 52 NDB strains belonging to seven genera were isolated from tobacco soil. The most dominant genera were Flavobacterium (36.54%), Pseudomonas (30.77%) and Arthrobacter (15.38%), and Chitinophaga and Flavobacterium have not been previously reported. Then, two efficient NDB strains, Arthrobacter nitrophenolicus ND6 and Stenotrophomonas geniculata ND16 were screened and inoculated in the compost fertilizer from tobacco waste. The nicotine content was reduced from 1.5 mg/g to below the safety threshold of 0.5 mg/g. Furthermore, strain ND6 followed the pyridine pathway of nicotine degradation, but the degrading pathway in strain ND16 could not be determined according to genomic analysis and color change. Finally, the abundance of nicotine-degrading genes in tobacco rhizosphere soil was investigated via metagenomic analysis. Five key genes, NdhA, nctB, KdhL, nboR, and Dhponh, represent the whole process of nicotine degradation, and their abundance positively correlated with soil nicotine content (p < 0.05). In conclusion, various NDB including unknown species live in tobacco soil and degrades nicotine effciently. Some key nicotine-degrading genes could be used in monitoring nicotine degradation in the environment. The fermentation of compost from tobacco waste is a promising application of efficient NDB.

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Improving the kinetic parameters of nicotine oxidizing enzymes by homologous structure comparison and rational design

Deay¹,

Seibold²,

Battaile³

et al. 2022

Archives of Biochemistry and Biophysics

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An active site mutation in 6-hydroxy-l-Nicotine oxidase from Arthrobacter nicotinovorans changes the substrate specificity in favor of (S)-nicotine

Cited by 4 publications

References 36 publications

Cultivation and application of nicotine-degrading bacteria and environmental functioning in tobacco planting soil

Cultivation and application of nicotine-degrading bacteria and environmental functioning in tobacco planting soil

Cultivation and application of nicotine-degrading bacteria and environmental functioning in tobacco planting soil

Improving the kinetic parameters of nicotine oxidizing enzymes by homologous structure comparison and rational design

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