An Activity‐Based Fluorescent Probe for Imaging Fluctuations of Peroxynitrite (ONOO⁻) in the Alzheimer's Disease Brain

Abstract

Peroxynitrite (ONOO À ) plays a critical role in Alzheimer's disease (AD). To reveal the ONOO À influx in AD brains, an activatable activity-based fluorescence probe Rd-DPA3 was designed by a structure-modulated strategy. Taking advantage of ONOO À -initiated twostep cascade reactions of a novel chemical trigger, Rd-DPA3 specifically responds to ONOO À in 0.3 mM of other reactive oxygen species (ROS) and varied proteins, and gives an intensive fluorescence enhancement (F/F 0 = 50). Moreover, with its mitochondria-targeting ability, Rd-DPA3 can be used to efficiently monitor the alternations of intracellular ONOO À levels in cerebral cells during oxidative stress. Significantly, due to NIR emission and good blood-brain barrier (BBB) crossing ability, Rd-DPA3 is suitable for in vivo imaging of cerebral ONOO À influx and illustrating an age-dependent accumulation of ONOO À in AD mice brains.

Recent advances in small molecular near-infrared fluorescence probes for a targeted diagnosis of the Alzheimer disease

“…10d) not only could cross BBB but also monitor the real-time in vivo imaging of ONOO − concentration fluctuation. 135…”

“…(d) Design of ONOO − -activated fluorescence probes. Schematic illustration of the current work for real-time imaging and monitoring ONOO − fluctuation in Alzheimer disease brain with an NIR fluorescence probe Rd-DPA3 135. Copyright 2022, Wiley Online Library.…”

A Tumor‐Specific Platform of Peroxynitrite Triggering Ferroptosis of Cancer Cells

“…This accumulation could be effectively suppressed by the Fer-1 treatment group, which was consistent with Fer-1 increasing cell viability shown before. Of note, a sharp decrease in the green fluorescence signals was observed when the cells were pretreated with uric acid (UA, an efficient ONOO − scavenger reagent), [21] indicating that LPO is caused by ONOO − .…”

Enhancing the Selectivity of Leucine Aminopeptidase Near-Infrared Fluorescent Probes for Assisting in Surgical Tumor Resection

“…Fluorescence imaging, especially near-infrared (NIR) fluorescence imaging, is a powerful tool for the in vivo imaging of targets with deep tissue penetration, minimal autofluorescence, and high resolution. − An activatable fluorescent probe is needed for the probe’s design to achieve workable information from biological applications. − An activatable NIR probe consists of a NIR fluorophore, linker, and recognition unit. The recognition unit regulates the probe selectivity and sensitivity.…”