An adhesive factor found in strains ofEscherichia coli belonging to the traditional infantile enteropathogenic serotypes

Cravioto, Alejandro; Gross, R. J.; Scotland, Sylvia M.; Rowe, B.

doi:10.1007/bf02602439

Cited by 594 publications

(394 citation statements)

References 23 publications

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“…Adhesion of EPEC to Caco-2 cells was carried out according to the method of Cravioto et al (1979) with slight modifications. Sub-confluent cell cultures on glass coverslips were placed in DMEM containing 0.5% mannose (w/v) and incubated with bacteria for 30 min.…”

Section: Caco-2 Cell Adherencementioning

confidence: 99%

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Role of bundle-forming pilus of enteropathogenicEscherichia coliin host cell adherence and in microcolony development

Tobe¹,

Sasakawa

2001

Cellular Microbiology

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SummaryEnteropathogenic Escherichia coli (EPEC) adheres to epithelial cells and forms microcolonies in localized areas. Bundle-forming pili (BFP) are necessary for autoaggregation and the formation of microcolonies. In this study, we show that BFP, expressed by EPEC on epithelial cells, disappeared with the expansion of the microcolony. Bacterial dispersal and the release of BFP from the EPEC aggregates were induced by contact with host cellular membrane extract. In addition, BFP-expressing EPEC adhered directly to cell surfaces, in preference to attaching to pre-formed microcolonies on the cells. These results suggested that BFP mediate the initial attachment of EPEC through direct interaction with the host cell rather than through the recruitment of unattached bacteria to microcolonies on the cell.

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Section: Caco-2 Cell Adherencementioning

confidence: 99%

“…EPEC colonizes the small intestine, and forms microcolonies on the intestinal surface (Cravioto et al, 1979;Nataro et al, 1985;1987). The initial adherence of EPEC is followed by intimate attachment, which leads to the formation of attaching and effacing (A/E) lesions Knutton et al, 1989).…”

Section: Introductionmentioning

confidence: 99%

Role of bundle-forming pilus of enteropathogenicEscherichia coliin host cell adherence and in microcolony development

Tobe¹,

Sasakawa

2001

Cellular Microbiology

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“…The presence of adhesive factors in all strains of E. coli isolated was studied with the HEp-2 assay designed by Cravioto et al (1979); adhesion to HEp-2 cells was classified as localized and diffuse according to Scaletzky, Silva & Trabulsi (1984). All strains were also tested for mannose-resistant haemagglutination (MRHA) with human group A and bovine erythrocytes .…”

Section: Diarrhoeal Disease In Mexican Childrenmentioning

confidence: 99%

Prospective study of diarrhoeal disease in a cohort of rural Mexican children: incidence and isolated pathogens during the first two years of life

Cravioto¹,

Reyes²,

Ortega³

et al. 1988

Epidemiol. Infect.

137

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SUMMARYColonization of the intestine by putative pathogens was followed longitudinally in a cohort of 56 infants born during one calendar year in a rural Mexican village with faecal cultures taken every fortnight and every time a child had diarrhoea. The frequency of isolation of pathogens during episodes of diarrhoea was compared with that of matched controls from the same cohort. Incidence of diarrhoea during the first year of life was 98%, diminishing to 93% during the second year. The incidence curves for each year were not significantly different (P > 041). Isolation of enteropathogenic Escherichia coli, enterotoxigenic Escherichia coli producing heat-stable (ST) and/or heat-labile (LT) enterotoxins and rotaviruses was significantly higher in infants with diarrhoea during the first 2 years of life. In the case of shigella, although no significant differences were found by semester of life, 13 of 16 children in which these strains were found had diarrhoea. Isolation of Salmonella spp., Campylobacter spp. and protozoa were not significantly different in the two groups during the period studied. Strains showing localized adherence to HEp-2 cells or the presence of colonization factor antigens I or E8775 were found with significantly higher frequency in children with diarrhoea. Eighty-two percent of ST' or LT' ETEC strains isolated produced one of the three known colonization factors.

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“…Haemagglutination of human erythrocytes by Aeromonas, which is not inhibited by fucose, galactose or mannose, shows a strong correlation with diarrhoeal isolates (Burke et al, 1984a), but the nature of the cell-associated haemagglutinin is not known. Adhesion to HEp-2 cells has recently provided a useful model for the study of pathogenicity in E. coli (Cravioto et al, 1979;Knutton et al, 1984;Tavendale and Old, 1985). Tissue culture adhesion assays are also reported for Salmonella (Mintz et al, 1983 ; Tavendale et a!., 1983), Campylobacter (Cinco et al, 1984) and Yersinia (Lassen and examined the association between this property and the presence of pili.…”

Section: Introductionmentioning

confidence: 99%

Adhesion of clinical and environmental Aeromonas isolates to HEp-2 cells

Carrello

Silburn²,

Budden³

et al. 1988

Journal of Medical Microbiology

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Summary.A total of 63 Aeromonas strains isolated from diarrhoeal faeces or water samples were tested for adhesion to HEp-2 cells. An association between diarrhoea and high level adhesion was observed in that 12 of the 34 faecal isolates and none of the 29 environmental isolates yielded >20 bacteria per HEp-2 cell in the adhesion assay. The proportion of high adherers was significantly greater for A . sobria (57%) than for A . hydrophila isolates (19%). Three of the eight faecal A . caviae isolates were also found to be high adherers. All of the environmental isolates were heavily pilated with pili having a mean diameter of 5 nm and a mean length of 420 nm; these were termed type-S pili. Of the 34 faecal isolates, 32% possessed S pili, and 68% were lightly pilated with up to 15 thin, flexible type-L pili, of mean diameter 2-5 nm and mean length 960 nm. Type-L pilation was associated with a high level of HEp-2 cell adhesion, and was more common in A . sobria and A . caviae than in A . hydrophila isolates. These results suggest that adherence to HEp-2 cells is a useful model for the investigation of Aeromonas enteropathogenicity, and that adhesion may be pilusmediated in this organism.

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An adhesive factor found in strains ofEscherichia coli belonging to the traditional infantile enteropathogenic serotypes

Cited by 594 publications

References 23 publications

Role of bundle-forming pilus of enteropathogenicEscherichia coliin host cell adherence and in microcolony development

Role of bundle-forming pilus of enteropathogenicEscherichia coliin host cell adherence and in microcolony development

Prospective study of diarrhoeal disease in a cohort of rural Mexican children: incidence and isolated pathogens during the first two years of life

Adhesion of clinical and environmental Aeromonas isolates to HEp-2 cells

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