An advanced loop-mediated isothermal amplification assay for the rapid detection of beak and feather disease virus in psittacine birds

Chae, Ha‐Gyeong; Lim, Da‐Rae; Kim, Hye‐Ryung; Park, Minji; Park, Choi‐Kyu

doi:10.1016/j.jviromet.2020.113819

Cited by 6 publications

(7 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The application of this simple sample pre-treatment method highlights the feasibility of LAMP in detecting target pathogens in different types of samples for a rapid diagnostic procedure. In a more recent development, the LAMP reaction has been incorporated with swarm primers to reduce the reaction time of BFDV detection to 40 min of complete reaction time and the results can be directly visualized with color changes of the hydroxy naphthol blue dye [ 71 ]. LAMP with swarm primers also demonstrated a great improvement in diagnostic performance by showing 100% clinical sensitivity from 71% of the standard LAMP method, following BFDV detection from psittacine samples of blood, feather, tissue and cloacal swabs [ 71 ].…”

Section: Lamp Application In Screening Of Avian Virusesmentioning

confidence: 99%

“…In a more recent development, the LAMP reaction has been incorporated with swarm primers to reduce the reaction time of BFDV detection to 40 min of complete reaction time and the results can be directly visualized with color changes of the hydroxy naphthol blue dye [ 71 ]. LAMP with swarm primers also demonstrated a great improvement in diagnostic performance by showing 100% clinical sensitivity from 71% of the standard LAMP method, following BFDV detection from psittacine samples of blood, feather, tissue and cloacal swabs [ 71 ]. Early and rapid diagnosis using the LAMP method to screen infected parrots prior to any international trade would help to control virus transmission [ 71 ].…”

Section: Lamp Application In Screening Of Avian Virusesmentioning

confidence: 99%

“…LAMP with swarm primers also demonstrated a great improvement in diagnostic performance by showing 100% clinical sensitivity from 71% of the standard LAMP method, following BFDV detection from psittacine samples of blood, feather, tissue and cloacal swabs [ 71 ]. Early and rapid diagnosis using the LAMP method to screen infected parrots prior to any international trade would help to control virus transmission [ 71 ].…”

Section: Lamp Application In Screening Of Avian Virusesmentioning

confidence: 99%

See 2 more Smart Citations

Loop-Mediated Isothermal Amplification (LAMP) as a Promising Point-of-Care Diagnostic Strategy in Avian Virus Research

Padzil

Mariatulqabtiah

Tan

et al. 2021

Animals

View full text Add to dashboard Cite

Over the years, development of molecular diagnostics has evolved significantly in the detection of pathogens within humans and their surroundings. Researchers have discovered new species and strains of viruses, while mitigating the viral infections that occur, owing to the accessibility of nucleic acid screening methods such as polymerase chain reaction (PCR), qualitative (real-time) polymerase chain reaction (qPCR) and reverse-transcription qPCR (RT-qPCR). While such molecular detection methods are widely utilized as the benchmark, the invention of isothermal amplifications has also emerged as a reliable tool to improvise on-field diagnosis without dependence on thermocyclers. Among the established isothermal amplification technologies are loop-mediated isothermal amplification (LAMP), recombinant polymerase amplification (RPA), strand displacement activity (SDA), nucleic acid sequence-based amplification (NASBA), helicase-dependent amplification (HDA) and rolling circle amplification (RCA). This review highlights the past research on and future prospects of LAMP, its principles and applications as a promising point-of-care diagnostic method against avian viruses.

show abstract

Section: Lamp Application In Screening Of Avian Virusesmentioning

confidence: 99%

Section: Lamp Application In Screening Of Avian Virusesmentioning

confidence: 99%

Section: Lamp Application In Screening Of Avian Virusesmentioning

confidence: 99%

See 1 more Smart Citation

Loop-Mediated Isothermal Amplification (LAMP) as a Promising Point-of-Care Diagnostic Strategy in Avian Virus Research

Padzil

Mariatulqabtiah

Tan

et al. 2021

Animals

View full text Add to dashboard Cite

show abstract

“…These include histology, electron microscopy, haemagglutination, [34][35] immunohistochemistry, [36] in situ hybridisation, [37] polymerase chain reaction (PCR), [38] duplex shuttle PCR, [39] real-time PCR, [40] PCR followed by high-resolution melting curve analysis, [15][20] and swarm primer-applied loop-mediated isothermal amplification (sLAMP). [41] The serological detection of anti-BFDV antibodies has been conducted by haemagglutination inhibition [34][42] and Enzyme-Linked Immunosorbent Assay (ELISA) [36] . So far, the standard PCR-based assay has been used most frequently (>49%) to screen BFDV between 1984 and July 2015.…”

Section: Diagnosismentioning

confidence: 99%

“…[18] A recently developed sLAMP assay may serve as a rapid, sensitive, and specific diagnostic field test for the detection of BFDV in clinical samples. [41]…”

Section: Diagnosismentioning

confidence: 99%

Beak and feather disease virus: biology and resultant disease

Sarker¹,

Forwood²,

Raidal³

2020

Wiki J Sci

View full text Add to dashboard Cite

The beak and feather disease virus (BFDV) causes psittacine beak and feather disease, an often chronic and fatal disease in psittacine birds. The virus most commonly infects psittacine birds, but is also capable of infecting nonpsittacine bird species in Australasia. The virus induces an immunosuppressive condition with chronic symmetrical irreversible loss of feather, as well as beak and claw deformities eventually leading to death. No specific treatment is currently commercially available for infected birds; however, a combination of quarantine and hygiene control, diagnostic testing and enhancing flock adaptive immunity is recommended to provide the most effective and sustainable control. Recent structural determination of BFDV capsid protein provides insights into the different assemblies that can be formed from one of the smallest known DNA viruses.

show abstract

Various Techniques for Molecular and Rapid Detection of Infectious and Epidemic Diseases

Shahrajabian

Sun

2023

LOC

View full text Add to dashboard Cite

Background: Polymerase chain reaction is an approach to make numerous copies of specific DNA. PCR has been applied for the investigation of infectious sicknesses caused by viral, protozoan, bacterial, fungal, or other infectious factors. Objective: This review manuscript aims to survey the usage of PCR, LAMP, RPA, and RAA in rapid detection and highlight molecular detection of various diseases and pathogens. Methods: Scientific sources like Science Direct, PubMed, Research gate, Scopus, and Google Scholar with highlighting on Science Direct and Scopus have been applied. A review of the literature was prepared by using the keywords PCR, LAMP, infectious disease, pathogen, RAA, RPA, and virus. Results: Loop-mediated isothermal amplification (LAMP) is a nucleic acid amplification method presenting the substitute to PCR. The LAMP assay is more rapid than nested PCR, is cost-efficient, and is simple to perform. LAMP technology has been widely used for the detection of crop pests, human pathogenic, pathogenic, organisms, bacteria, and components in meat products. Recombinase polymerase amplification (RPA) is a new isothermal technique to amplify the DNA as well as RPA. RPA combined the advantages of isothermal PCR with clarity and rapid amplification. Recombinase-aided amplification (RAA) assay has been successfully applied in the detection of bacterial and viral pathogens and controls the technical problems posed by DNA amplification techniques because it does not require thermal denaturation of the template and utilizes at a debilitated and continuous temperature. Conclusion: This manuscript has highlighted the importance of PCR and molecular detection as significant tools in the detection of infectious organisms, pathogens, toxins, and biological research.

show abstract

An advanced loop-mediated isothermal amplification assay for the rapid detection of beak and feather disease virus in psittacine birds

Cited by 6 publications

References 28 publications

Loop-Mediated Isothermal Amplification (LAMP) as a Promising Point-of-Care Diagnostic Strategy in Avian Virus Research

Loop-Mediated Isothermal Amplification (LAMP) as a Promising Point-of-Care Diagnostic Strategy in Avian Virus Research

Beak and feather disease virus: biology and resultant disease

Various Techniques for Molecular and Rapid Detection of Infectious and Epidemic Diseases

Contact Info

Product

Resources

About