An affordable, handheld multimodal microscopic system with onboard cell morphology and counting features on a mobile device

Rabha, Diganta; Biswas, Sritam; Hatiboruah, Diganta; Das, Priyanka; Rather, Muzamil Ahmad; Mandal, Manabendra; Nath, Pabitra

doi:10.1039/d1an02317a

Cited by 7 publications

(2 citation statements)

References 37 publications

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“…Hence, imaging methods that capture rich cell morphologies have attracted much attention in the mobile blood analysis field with the advancement of optical and machine-vision techniques. [34][35][36][37][38][39] But most imaging blood analysis methods use complex or susceptible sample preparations, including fluorescence staining and density centrifugation, to achieve WBC subtype differential. Label-free imaging techniques, such as deep-ultraviolet microscopy [40][41][42] and lens-free microscopy, 43,44 can solve this problem by characterizing WBCs with their endogenic properties.…”

Section: Introductionmentioning

confidence: 99%

Multiparameter mobile blood analysis for complete blood count using contrast-enhanced defocusing imaging and machine vision

Chen

Zeng

et al. 2023

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Section: Introductionmentioning

confidence: 99%

Multiparameter mobile blood analysis for complete blood count using contrast-enhanced defocusing imaging and machine vision

Chen

Zeng

et al. 2023

Analyst

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“…Thus, imaging methods that capture cell morphology have attracted much attention in the mobile blood analysis field with the advancement of optical and machine-vision techniques [34][35][36][37][38][39][40] . Many works reported mobile phone microscopes for blood cell applications, including partial WBC differential combined with fluorescence staining or density centrifugation 41,42 .…”

Section: Introductionmentioning

confidence: 99%

Multiparameter mobile blood analyzer for complete blood count utilizing contrast-enhanced defocusing imaging

Chen

et al. 2022

Preprint

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Blood analysis, though complete blood count (CBC), is the most basic medical test for disease diagnosis and health monitoring. However, the need for bulky and expensive laboratory facilities and skilled technicians limits the universal medical practices based on blood analysis outside of well-equipped laboratory environments. Here, we proposed a multiparameter mobile blood analyzer utilizing label-free contrast-enhanced defocusing imaging (CEDI) with the assistance of machine vision for instant and on-site blood analysis. The analyzer using CEDI obtains both the blood cell morphological characteristics and hemoglobin spectrophotometric information for simultaneous five-part white blood cell differential count, red blood cell count, and hemoglobin quantification without the need for sample staining. In addition, we have designed a miniature microscope with a sub-micron spatial resolution of (~0.98 μm), which is quite small, lightweight, and cost-effective for blood imaging. We have shown that our assay can analyze a sample within 10 minutes, using just a drop of fingertip blood (~7 μl) and measurements (30 samples) from the analyzer have strong correlations with clinical reference values (significant level: P < 0.0001). The proposed mobile blood analyzer can help provide universal access to blood analysis and has great potential for integrated surveillance of various epidemic diseases, including coronavirus infection, invermination, and anemia, especially in low-and middle-income countries.

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Notizen aus der Chemie

Delaittre¹,

Dierkes²,

Heine³

et al. 2022

Nachr Chem

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Notizen aus der ChemieSmartphone-Mikroskop | | Rabha und Kolleg:innen haben ein Smartphone-basiertes Bright-field-Fluoreszenzmikroskop entwickelt. Das System nutzt die Kamera des Smartphones in Kombination mit optischen Bauteilen, welche die Autoren in 3-D-gedruckte Formteile einbauen. Das System ermöglicht eine laterale Auflösung von etwa 1,2 µm mit drei verschiedenen Vergrößerungsstufen bis zum knapp 40-Fachen. Zudem haben die Autoren einen Algorithmus entwickelt, der Zellen zählt. Testanwendung war das Imaging roter Blutzellen.Das einfache System eignet sich etwa für medizinische Anwendungen in Krisenregionen oder Schwellenländern. BM

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An affordable, handheld multimodal microscopic system with onboard cell morphology and counting features on a mobile device

Abstract: A simple yet effective, handheld and flexible bright-field and fluorescence microscopic platform on a smartphone with varying optical magnifications is reported for morphological analysis and onboard cell counting features.

Cited by 7 publications

References 37 publications

Multiparameter mobile blood analysis for complete blood count using contrast-enhanced defocusing imaging and machine vision

Multiparameter mobile blood analysis for complete blood count using contrast-enhanced defocusing imaging and machine vision

Multiparameter mobile blood analyzer for complete blood count utilizing contrast-enhanced defocusing imaging

Notizen aus der Chemie

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