An alcohol oxidase-based electrochemical sensor for the rapid determination of lower alcohols

Алферов, В. А.; Zaitsev, M.G.; Ponomareva, Olga N.; Кузнецова, Т. А.; Rogova, Tatiana; Reshetilov, A. N.

doi:10.1134/s1061934811100030

Cited by 10 publications

(6 citation statements)

References 12 publications

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“…The high response of the biosensor to formaldehyde is presumably determined by the non-specific oxidation of the hydrated formaldehyde species (geminal diol) by MDH [ 54 ]. Being simple and economical in fabrication, MDH-His 6 -tag-biosensor does not give way to other methanol biosensors [ 55 , 56 , 57 ], and even surpasses them in some analytical and metrological characteristics.…”

Section: Discussionmentioning

confidence: 99%

Isolation and Characterization of Homologically Expressed Methanol Dehydrogenase from Methylorubrum extorquens AM1 for the Development of Bioelectrocatalytical Systems

Karaseva

Федоров

Baklagina

et al. 2022

IJMS

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(Ca2+)-dependent pyrroloquinolinequinone (PQQ)-dependent methanol dehydrogenase (MDH) (EC: 1.1.2.7) is one of the key enzymes of primary C1-compound metabolism in methylotrophy. PQQ-MDH is a promising catalyst for electrochemical biosensors and biofuel cells. However, the large-scale use of PQQ-MDH in bioelectrocatalysis is not possible due to the low yield of the native enzyme. Homologously overexpressed MDH was obtained from methylotrophic bacterium Methylorubrum extorquens AM1 by cloning the gene of only one subunit, mxaF. The His-tagged enzyme was easily purified by immobilized metal ion affinity chromatography (36% yield). A multimeric form (α6β6) of recombinant PQQ-MDH possessing enzymatic activity (0.54 U/mg) and high stability was demonstrated for the first time. pH-optimum of the purified protein was about 9–10; the enzyme was activated by ammonium ions. It had the highest affinity toward methanol (KM = 0.36 mM). The recombinant MDH was used for the fabrication of an amperometric biosensor. Its linear range for methanol concentrations was 0.002–0.1 mM, the detection limit was 0.7 µM. The properties of the invented biosensor are competitive to the analogs, meaning that this enzyme is a promising catalyst for industrial methanol biosensors. The developed simplified technology for PQQ-MDH production opens up new opportunities for the development of bioelectrocatalytic systems.

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Section: Discussionmentioning

confidence: 99%

Isolation and Characterization of Homologically Expressed Methanol Dehydrogenase from Methylorubrum extorquens AM1 for the Development of Bioelectrocatalytical Systems

Karaseva

Федоров

Baklagina

et al. 2022

IJMS

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“…Alferov et al developed methanol sensors by using a bio-selective membrane composed of four yeast alcohol oxides. 45 The sensor required a complex fabrication process and output signal registration. Xu et al demonstrated a thin-film ethanol sensor based on graphene nanosheets (GNs) and bacterial cellulose nanofibers.…”

Section: ■ Introductionmentioning

confidence: 99%

“…One common type of sensor used for this purpose is electrochemical sensors. The basic component of these sensors include enzymes like alcohol dehydrogenase and alcohol oxidase. − For instance, Biscay et al reported an electrochemical sensor for ethanol monitoring in sweat . The sensor utilized polyaniline-modified screen-printed carbon electrodes for the oxidation of ethanol in artificial sweat.…”

Section: Introductionmentioning

confidence: 99%

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Nanostructured Photonic Hydrogels for Real-Time Alcohol Detection

Ahmed

Elsherif

Park

et al. 2022

ACS Appl. Nano Mater.

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Rapid, reliable, and reusable sensors with a low limit of detection can play a vital role in alcohol detection, which is vital in a myriad of industries. Herein, hydrogel-based fiber optic probes were developed for alcohol sensing by a simple, rapid, and facile process. Aztec microstructures with a periodicity of 3 μm were imprinted onto alcohol-sensitive hydrogels for fabricating stand-alone and fiber optic sensors. The hydrogel matrix undergoes swelling in response to alcohol solutions, altering its diffraction efficiency. The real-time sensing capability of the developed sensors was demonstrated in response to various concentrations of ethanol, isopropanol, methanol, and dimethyl sulfoxide in the concentration range of 0 to 50 vol %. For the stand-alone sensors, sensitivities of 23, 21, and 15 μW/vol % were recorded for dimethyl sulfoxide (DMSO), isopropanol, and ethanol, respectively. For the optical fiber probes, sensitivities of 2.3, 2.2, 1.94, and 1.14 μW/vol % were recorded for DMSO, isopropanol, ethanol, and methanol, respectively. A fast response time of 20 s was recorded for both stand-alone and optical fiber sensors. Higher sensitivity toward DMSO was observed, with a low limit of detection of 0.5 vol %. The developed optical fiber sensors may have applications in remote and real-time alcohol sensing in forensics, medicine, food, and beverage manufacturing industries.

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“…Therefore, a new biosensorbased electrochemical method has been developed to detect ethanol (Shkotova et al 2005;Carelli et al 2006;Türkarslana et al 2010;Rama et al 2012;Cinti et al 2017). The biosensors has several advantages such as faster, simple, inexpensive, sensitive, selective techniques, and can be miniaturized (Alferov et al 2011). As such, alcohol biosensors based on alcohol oxidase (Rama et al 2012;Kurbanoglu and Toppare 2014;Chinnadayyala et al 2015) and alcohol dehydrogenase (Bilgi and Ayranci 2016;Alpat and Telefoncu 2010) have been developed.…”

Section: Introductionmentioning

confidence: 99%

Activity and Stability of the Alcohol Biosensor Using Acetobacter aceti Biofilm on Screen-Printed Carbon Electrode

et al. 2020

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Most of the alcohol analytical methods are robust and instrumentally expensive. An alternative of ethanol biosensor based on selected biofilm forming Acetobacter aceti bacteria producing alcohol oxidase was constructed on a screen-printed carbon electrode. The enzyme specifically oxidizes the ethanol and generate electrical current that then electrochemically detected and measured by cyclic voltammetry method. A scanning electron microscopic analysis indicated that the biofilm was formed firmly in the electrode. This constructed biosensor reached its optimum at biofilm formed by bacteria of 1.33 × 1010 cells/ml, temperature of 27°C, and pH 7. The enzyme kinetic had KM and Vmax AOX values of 3.5 mm and 125 μA respectively. The biosensor had detection and quantization limit of 0.003 and 0.009%, respectively, and a sensitivity of 57.29 μA (%)-1. A linearity and relative deviation value were revealed at 0.993 and 1.95% respectively. The biosensor was relatively specific and had no interferences with methanol, sodium chloride and citric acid as the common interferences of ethanol compounds. Furthermore, the biosensor had been stably for at least 55 days. Therefore, this constructed biosensor should be developed into a prototype for a practical effective analysis.

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An alcohol oxidase-based electrochemical sensor for the rapid determination of lower alcohols

Cited by 10 publications

References 12 publications

Isolation and Characterization of Homologically Expressed Methanol Dehydrogenase from Methylorubrum extorquens AM1 for the Development of Bioelectrocatalytical Systems

Isolation and Characterization of Homologically Expressed Methanol Dehydrogenase from Methylorubrum extorquens AM1 for the Development of Bioelectrocatalytical Systems

Nanostructured Photonic Hydrogels for Real-Time Alcohol Detection

Activity and Stability of the Alcohol Biosensor Using Acetobacter aceti Biofilm on Screen-Printed Carbon Electrode

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