An allelic typing method for 2DS4 variant used in study of haplotypes of killer cell immunoglobulin‐like receptor gene

Bao, Xiaojing; Hou, Lihua; Sun, Aining; Chen, M.; Chen, Z.; He, Jun

doi:10.1111/j.1751-553x.2010.01234.x

Cited by 12 publications

(5 citation statements)

References 27 publications

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“…For 2DS3/S5 that can be located on both centrometric and telomeric sides, the gene was assigned basically on identified KIR gene motifs and the linkage between 2DL5A/B and 2DS3/S5 . In‐house locus specific primers were used to confirm the presence or absence of KIR3DS1 and deleted‐KIR2DS4 genes as there are occasionally weak PCR reactions for the corresponding wells using the commercial Kits .…”

Section: Methodsmentioning

confidence: 99%

“…In humans, a unique feature of the KIR system is the segregation of two distinctive groups of haplotypes (A and B), which are present in all the >150 human populations examined and are maintained by balancing selection . Some recent studies have indicated that patients transplanted from donors carrying at least one B haplotype had a better clinical outcome compared with those transplanted from a donor homozygous for KIR haplotype A . These data highlight the effect of KIR haplotypes in allo‐HSCT.…”

Section: Introductionmentioning

confidence: 97%

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Characterization of Killer cell immunoglobulin‐like receptor (KIR) genotypes and haplotypes in Chinese Han population

et al. 2013

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We performed Killer cell immunoglobulin-like receptor (KIR) genotyping on 1271 individuals of Chinese Han origin including 102 families and 965 unrelated individuals. The families (with one child and both parents) were subjected for haplotype analysis. Forty-one different genotypes were identified. The frequencies of the KIR genotypes found in the family panel were confirmed by those found in the unrelated panel. The family study showed segregation of one A haplotype and at least 15 unique B haplotypes. The most commonly observed haplotypes in group B were B1, B2, and B3, present at a frequency of 10.05%, 6.62%, and 4.90%, respectively. On the basis of the combination of KIR genes, six centromeric and seven telomeric gene motifs have been identified. Motif cB02 was the most frequent haplotype B specific centromeric segment while tB01 was the most frequent haplotype B specific telomeric segment. The distinct distribution of KIR haplotypes in each population may reflect the history of directional and balancing selection of different races. The gene combinations of group A and B1/B2/B3 haplotype were the most frequent genotypes named as Bx1, Bx2, and Bx3, present at a frequency of 13.72%, 7.35%, and 4.41% in the family panel, and at a frequency of 15.86%, 10.15%, and 5.80% in the unrelated panel, respectively. Overall, this study showed the diversity of KIR haplotypes and genotypes in Chinese Han population and developed a criterion for distinguish KIR haplotypes/genotypes for the population. KIR genotyping and haplotype analysis should be useful for selection of the most optimum donor grafts with favorable KIR gene content for transplants.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%

Characterization of Killer cell immunoglobulin‐like receptor (KIR) genotypes and haplotypes in Chinese Han population

et al. 2013

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“…(2004) showed that women who belong to haplotype A with a KIR1D may have less reproductive success. Bao et al. (2010) reported that more KIR1D allele in donor contributed to a lower risk of acute graft‐versus‐host disease in haplotype A group and led to a good clinical outcome.…”

Section: Discussionmentioning

confidence: 99%

Association of KIR2DS4 and its variant KIR1D with syphilis in a Chinese Han population

Zhuang¹,

Zhu²,

Zhang³

et al. 2011

Int J Immunogenetics

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Syphilis is a sexually transmitted infection caused by the Treponema pallidum subspecies pallidum spirochaete bacterium. The killer immunoglobulin-like receptor (KIR) gene family encodes cell surface receptors that are found on natural killer (NK) cells and certain T-cell subsets. NK cells are fast-acting effector lymphocytes of innate immunity that respond to infection. The activity of NK cells depends on the dynamic balance of activating and inhibitory signals that are transmitted through respective receptors including KIRs. KIR2DS4 is the only activating KIR gene in KIR haplotype A. KIR1D is a partial deletion KIR2DS4 variant encoding protein devoid of transmembrane region. Up to now, there is no knowledge of association of KIR2DS4 and its variant KIR1D with syphilis in a population that belongs to KIR gene haplotype A. Polymerase chain reaction with sequence-specific primers (PCR-SSP) method was used to genotype KIR genes in 190 patients with syphilis and 192 healthy controls. The gene frequencies of KIR2DS4 and KIR1D were analysed for the association with syphilis in patients and healthy controls who belong to KIR gene haplotype A. The gene frequency of KIR1D/KIR1D in patients with syphilis classified as haplotype A was 16.85% and was significantly higher than that in the control group (6.59%) (P = 0.032). However, there was no significant difference for the gene frequencies of KIR2DS4/KIR2DS4 and KIR2DS4/KIR1D between the two groups (P > 0.05). KIR1D/KIR1D was found in association with susceptibility to syphilis in the Chinese Han population that belongs to KIR gene haplotype A.

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“…As one of the tumors with the highest incidence in China, the related research results show that (1) at present, the incidence of leukemia is increasing year by year (2). Currently, there is a high incidence of leukemia, especially acute leukemia, which is a cancer that progresses rapidly (3).…”

Section: Introductionmentioning

confidence: 99%

Study on the association of the polymorphism of HLA-II gene with leukemia

Song

Chen

et al. 2017

Oncology Letters

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We explored the association between the HLA-II gene polymorphisms and the occurrence of leukemia. For this study, we selected 53 patients with leukemia treated at Zhongnan Hospital of Wuhan University from February 2014 to September 2015 and 46 healthy patients as the control group. We used polymerase chain reaction with sequence specific primers for DNA typing which was carried out to analyze the patients HLA-A/B gene polymorphism. We also used enzyme-linked immunosorbent assay and western blotting method to measure the protein expression of different genotypes and activity. Compared to the control group, HLA-A04, B08 gene frequencies were significantly lower than those of HLA-A04, B08 gene frequencies of the observation group; results were statistically significant (χ2=16.28, P<0.05; χ2=16.47, P<0.05). However, in the control group, the frequency of HLA-A09 gene was significantly higher than that of the observation group; there was a significant difference between the two groups (χ2=15.28, P<0.05). Through the measurement of the protein expression levels of the different genotypes in the control group and the observation group, it was found that in the observation group, HLA-A04, B08 protein contents (4.6 and 3.2 µg/l) were significantly higher than those of the control group (0.13 and 0.1 µg/l). While the control group HLA-A09 genotype protein content (3.7 µg/l) was significantly higher than that of the observation group (0.2 µg/l); there were significant differences between both (P<0.05). Therefore, there is a significant correlation between HLA-II gene polymorphism and leukemia that is higher than HLA-A04 and B08 gene frequency and can help promote the occurrence of leukemia. The higher frequency of HLA-A09 gene can help to suppress the occurrence of leukemia.

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An allelic typing method for 2DS4 variant used in study of haplotypes of killer cell immunoglobulin‐like receptor gene

Cited by 12 publications

References 27 publications

Characterization of Killer cell immunoglobulin‐like receptor (KIR) genotypes and haplotypes in Chinese Han population

Characterization of Killer cell immunoglobulin‐like receptor (KIR) genotypes and haplotypes in Chinese Han population

Association of KIR2DS4 and its variant KIR1D with syphilis in a Chinese Han population

Study on the association of the polymorphism of HLA-II gene with leukemia

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