An alternative in vitro method for testing the potency of the polyvalent antivenom produced in Costa Rica

Gutiérrez, José Marı́a; Avila, Claudio; Rojas, Ermila; Cerdas, Luis

doi:10.1016/0041-0101(88)90010-4

Cited by 286 publications

(151 citation statements)

References 5 publications

Supporting

Mentioning

133

Contrasting

Unclassified

Order By: Relevance

“…Incubation of the venom at different temperatures indicated that the venom component responsible for hemolysis is thermolabile. Other biological activities of B. lanceolatus, such as rat paw edema, leukocyte migration and hemorrhagic activity, are also thermolabile (7,21,22 (16,(28)(29)(30)(31)(32).…”

Section: Discussionmentioning

confidence: 99%

In vitro hemolytic activity of Bothrops lanceolatus (fer-de-lance) venom

Martins¹,

Araújo²,

Bon³

et al. 2009

J. Venom. Anim. Toxins incl. Trop. Dis

View full text Add to dashboard Cite

Bothrops lanceolatus venom contains a variety of enzymatic and biological activities. The present work investigated the hemolytic activity of this venom and its phospholipase A 2 (PLA 2 ). Bothrops lanceolatus venom (6.7 µg/mL) caused indirect hemolysis of cow, horse, rat and sheep erythrocytes, with horse erythrocytes being the most sensitive; no direct hemolysis was observed. Hemolysis in sheep erythrocytes was concentration-dependent (5-11.7 µg/mL) and markedly attenuated by heating the venom for 30 minutes at ≥ 40°C and by the PLA 2 inhibitor p-bromophenacyl bromide. An acidic PLA 2 (5 µg/mL) purified from B. lanceolatus venom also caused hemolysis. This PLA 2 showed immunoprecipitin lines with antivenom against B. lanceolatus, which suggests that the enzymatic and hemolytic activities of this enzyme may be neutralized during antivenom therapy. These results indicate that B. lanceolatus venom and its PLA 2 can cause hemolysis in vitro.

show abstract

Section: Discussionmentioning

confidence: 99%

In vitro hemolytic activity of Bothrops lanceolatus (fer-de-lance) venom

Martins¹,

Araújo²,

Bon³

et al. 2009

J. Venom. Anim. Toxins incl. Trop. Dis

View full text Add to dashboard Cite

show abstract

“…Phospholipase A2 activity was measured using an indirect hemolytic assay on agarose-erythrocyte-egg yolk gel plate by the methods described by Gutierrez et al, 1988. Increasing doses of cobra and krait venom (µg) was added to 3 mm wells in agarose gels (0.8% in PBS, pH 8.1) containing 1.2% sheep erythrocytes, 1.2% egg yolk as a source of lecithin and 10 mM CaCl2.…”

Section: Phospholipase Activitymentioning

confidence: 99%

Antitoxin activity of Mimosa pudica root extracts against Naja naja and Bangarus caerulus venoms

Meenachisundharam¹,

Priyagrace²,

Vijayaraghavan³

et al. 2009

Bangladesh J Pharmacol

View full text Add to dashboard Cite

Aqueous extract of dried roots of Mimosa pudica was tested for inhibitory activity on lethality, phospholipase activity, edema forming activity, fibrinolytic activity and hemorrhagic activity of Naja naja and Bangarus caerulus venoms. The aqueous extract displayed a significant inhibitory effect on the lethality, phospholipase activity, edema forming activity, fibrinolytic activity and hemorrhagic activity. About 0.14 and 0.16 mg of M. pudica extracts were able to completely neutralize the lethal activity of 2LD50 of Naja naja and Bangarus caerulus venoms respectively. The present finding suggests that aqueous extract of M. pudica root possesses compounds, which inhibit the activity of Naja naja and Bangarus caerulus venoms.

show abstract

“…These lysophospholipids have membrane activity over red blood cells, producing hemolysis that can be detected through the quantification of hemoglobin present in solution or through a hemolysis halo present in agarose gel containing intact red blood cells [76].…”

Section: Indirect Hemolysismentioning

confidence: 99%