An alternative way to encapsulate probiotics within electrospun alginate nanofibers as monitored under simulated gastrointestinal conditions and in kefir

Investigation of Lactobacillus paracasei encapsulation in electrospun fibers of Eudragit® L100

“…As shown from comparison with data reported in the literature, superior performance in terms of survivability degree of cells during the electrospun procedure and the lower reduction in the viable cells at SGF/ SIF conditions is reached for binary systems, such as introduced as Yilmaz et al [17] that associated conventional polymer matrix and sodium alginate.…”

“…The poor survival rate of bioactive cells can be attributed to environmental conditions such as acidic medium, the toxicity of oxygen and UV light [12][13][14][15][16] . Healthy-promoting effects of probiotics are extremely dependent on cell viability degree and the concentration of living cells as high as 10 9 CFU/ day for administration [17] . To circumvent the drawbacks related to low shelf-life of food products and adverse conditions at the acidic environment (stomach/ bile salts) the creation of an anaerobic environment for probiotics growth received increased attention in the literature with promising strategies to maintain the viability of cells until to reach the colon lumen.…”

“…To circumvent the drawbacks related to low shelf-life of food products and adverse conditions at the acidic environment (stomach/ bile salts) the creation of an anaerobic environment for probiotics growth received increased attention in the literature with promising strategies to maintain the viability of cells until to reach the colon lumen. These encapsulation strategies are based on the production of fruit bubles [18] , nanoencapsulation by electrospinning [17,19] and by the production of microcapsules [20,21] .…”

Encapsulating bacteria in alginate-based electrospun nanofibers

“…44 The encapsulation and delivery of gut probiotic bacteria from PVA and PEO fibers has also been demonstrated. 17,45,47 However, research on the encapsulation of microbes into nanofibers fabricated from the generally regarded as safe (GRAS) biopolymer alginate is scarce; to date the authors are only aware of one report 48 (that was published while we were writing this manuscript).…”

Nanomaterial-based encapsulation for controlled gastrointestinal delivery of viable probiotic bacteria

“…Encapsulation by alginatebased electrospun nanober mats has been shown to protect probiotic bacteria from the harsh gastrointestinal environment. A viability rate of 64.1 to 70.8 log CFU mL À1 has been achieved for Lactobacillus paracasei KS-199 encapsulated by alginate-based nanobers in simulated gastrointestinal uids 102. Electrospun nanobers based on gum arabic/ pullulan are another new type of material that can be used for the protection of probiotic cells.…”