An Amperometric Biosensor for Glucose Based on a Composite Electrode of Glucose Dehydrogenase, Carbon Nanotubes, and Plasma-polymerized Thin Films

Muguruma, Hitoshi; Yoshida, Shosuke; Urata, Muneyuki; Fujisawa, Kohta; Matsui, Yasunori

doi:10.5796/electrochemistry.76.545

Cited by 6 publications

(4 citation statements)

References 33 publications

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“…The increase in current at ca. +0.6 V can be assigned to eqs and . This peak for the sSWNT-GDH electrode is more distinct than that for the mSWNT-GDH electrode.…”

Section: Resultsmentioning

confidence: 91%

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Electronically Type-Sorted Carbon Nanotube-Based Electrochemical Biosensors with Glucose Oxidase and Dehydrogenase

Muguruma

Hoshino

Nowaki

2014

ACS Appl. Mater. Interfaces

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An electrochemical enzyme biosensor with electronically type-sorted (metallic and semiconducting) single-walled carbon nanotubes (SWNTs) for use in aqueous media is presented. This research investigates how the electronic types of SWNTs influence the amperometric response of enzyme biosensors. To conduct a clear evaluation, a simple layer-by-layer process based on a plasma-polymerized nano thin film (PPF) was adopted because a PPF is an inactive matrix that can form a well-defined nanostructure composed of SWNTs and enzyme. For a biosensor with the glucose oxidase (GOx) enzyme in the presence of oxygen, the response of a metallic SWNT-GOx electrode was 2 times larger than that of a semiconducting SWNT-GOx electrode. In contrast, in the absence of oxygen, the response of the semiconducting SWNT-GOx electrode was retained, whereas that of the metallic SWNT-GOx electrode was significantly reduced. This indicates that direct electron transfer occurred with the semiconducting SWNT-GOx electrode, whereas the metallic SWNT-GOx electrode was dominated by a hydrogen peroxide pathway caused by an enzymatic reaction. For a biosensor with the glucose dehydrogenase (GDH; oxygen-independent catalysis) enzyme, the response of the semiconducting SWNT-GDH electrode was 4 times larger than that of the metallic SWNT-GDH electrode. Electrochemical impedance spectroscopy was used to show that the semiconducting SWNT network has less resistance for electron transfer than the metallic SWNT network. Therefore, it was concluded that semiconducting SWNTs are more suitable than metallic SWNTs for electrochemical enzyme biosensors in terms of direct electron transfer as a detection mechanism. This study makes a valuable contribution toward the development of electrochemical biosensors that employ sorted SWNTs and various enzymes.

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“…The increase in current at ca. +0.6 V can be assigned to eqs and . This peak for the sSWNT-GDH electrode is more distinct than that for the mSWNT-GDH electrode.…”

Section: Resultsmentioning

confidence: 91%

“…+0.6 V can be assigned to eqs 6 and 7. 39 This peak for the sSWNT-GDH electrode is more distinct than that for the mSWNT-GDH electrode. The current of the sSWNT-GDH electrode is 4 times larger than that of the mSWNT-GDH counterpart (Figure 3A−C).…”

Section: Acs Applied Materials and Interfacesmentioning

confidence: 92%

Electronically Type-Sorted Carbon Nanotube-Based Electrochemical Biosensors with Glucose Oxidase and Dehydrogenase

Muguruma

Hoshino

Nowaki

2014

ACS Appl. Mater. Interfaces

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“…4 The selectivity of these SWCNT-based sensors can be modulated by functionalizing the surface of the nanotubes with proteins, such as glucose-binding protein, glucose dehydrogenase, concanavalin A, or glucose oxidase (GOx). [5][6][7][8] Among these proteins, GOx represents the gold standard for glucose sensing because of its stability and high specicity to glucose. 9 The oxidation of glucose and release of free electrons occur at the reaction center of the protein in the presence of a avin adenine dinucleotide (FAD) cofactor.…”

Section: Introductionmentioning

confidence: 99%

Bioengineering a glucose oxidase nanosensor for near-infrared continuous glucose monitoring

et al. 2022

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“…It was also reported previously that MWNTs can provide a favorable microenvironment for redox proteins to exchange electrons directly with underlying electrodes. 16 Many investigations showed that MWNTs/Nafion composite membrane was able to electrocatalyze the redox of protein compared with bare GCE. In order to facilitate comparison, MWNT/Nafion/GCE and bare edge-plane pyrolytic graphite electrodes are selected in investigation.…”

Section: Introductionmentioning

confidence: 99%

Electrochemistry of Eu(III) Binding to N-terminal of Euplotes Octocarinatus Centrin

Rong

Liu

et al. 2014

Electrochemistry

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Ciliate Euplotes octocarinatus centrin (EoCen) is a member of the EF-hand super family of calcium-binding proteins, which is associated with the centrosomes. In this work, the interaction between N-terminal of EoCen (N-EoCen) and Eu 3+ was investigated by cyclic voltammetry and UV-Vis spectroscopies. The result shows that a 2:1 of Eu 3+ to N-EoCen complex (Eu 2 -N-EoCen) is formed. The electrochemical characteristics of Eu 2 -N-EoCen at a multi-wall carbon nanotube/Nafion composite film modified glassy carbon electrode (MWNT/Nafion/GCE) were then investigated in detail, from which a direct, quasi reversible electron transfer of Eu 2 -N-EoCen had been obtained. According to the Laviron diffusionless-controled equations, the electron transfer rate constant k s , was calculated to be 2.33 + 0.14 s −1 . The pH dependence of the formal potential of Eu 2 -N-EoCen suggests that protonation accompanies electron transfer between pH 5.5-7.4. The electrochemical redox and quantitative analysis of Eu 2 -NEoCen are therefore of great significance not only studying the physiological processes of Eu 2 -N-EoCen in organisms but also the interaction mechanism with target protein.

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An Amperometric Biosensor for Glucose Based on a Composite Electrode of Glucose Dehydrogenase, Carbon Nanotubes, and Plasma-polymerized Thin Films

Cited by 6 publications

References 33 publications

Electronically Type-Sorted Carbon Nanotube-Based Electrochemical Biosensors with Glucose Oxidase and Dehydrogenase

Electronically Type-Sorted Carbon Nanotube-Based Electrochemical Biosensors with Glucose Oxidase and Dehydrogenase

Bioengineering a glucose oxidase nanosensor for near-infrared continuous glucose monitoring

Electrochemistry of Eu(III) Binding to N-terminal of Euplotes Octocarinatus Centrin

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